Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 16  |  Issue : 72  |  Page : 745--749

Simultaneous determination of two diterpenoids, continentalic acid and kaurenoic acid, in the water extract of Aralia continentalis and their wound-healing activity


Guijae Yoo1, Taek Hwan Lee2, Jae Hyuk Lee3, Kyo Bin Kang4, Heejung Yang5, Yong Kon Park1, Sun Yeou Kim3, Seung Hyun Kim2 
1 Korea Food Research Institute, Jeollabuk-do, Korea
2 College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon, Korea
3 College of Pharmacy, Gachon University, Incheon, Korea
4 College of Pharmacy, Sookmyung Women's University, Seoul, Korea
5 College of Pharmacy, Kangwon National University, Chuncheon, Korea

Correspondence Address:
Seung Hyun Kim
#421 Veritas Hall D, Yonsei Univ. 85 Songdogwahak-ro, Yeonsu-gu, Incheeon 406-840
Korea

Background: The roots of Aralia continentalis have been used in traditional Korean medicine to treat pain, lumbago, lameness, and rheumatism. Objective: Simultaneous determination of two diterpenoids; continentalic acid (CA) and kaurenoic acid, in the water extract of A. continentalis using the high-performance liquid chromatography (HPLC) was established for quality control, as well as evaluation of their wound-healing activity. Materials and Methods: The separation was conducted on YMC hydrosphere C18 column with isocratic elution of 65% acetonitrile. Furthermore, the isolated diterpenoids were screened for their wound-healing activity using keratinocytes (HaCaT cells). Results: The calibration curves were linear over the established range with R2 > 0.9999. The intraday and interday RSDs for each compound were 0.13%–0.89% and 0.14%–0.73%, respectively. The limits of detection and limits of quantification for the two tested diterpenoids were 0.0912, 0.0018 μg/μL, and 0.2764, 0.0056 μg/μL, respectively. In addition, the CA-treated groups showed accelerated wound closure compared to the control group. Conclusion: The HPLC method could be accomplished to the quality control and stable experiment for the preparations consisted of two diterpenoids. Compared to the control group, the CA-treated group showed that wound closure was accelerated, indicating its potential in promoting migration of skin cells which is the most important step of wound closure.


How to cite this article:
Yoo G, Lee TH, Lee JH, Kang KB, Yang H, Park YK, Kim SY, Kim SH. Simultaneous determination of two diterpenoids, continentalic acid and kaurenoic acid, in the water extract of Aralia continentalis and their wound-healing activity.Phcog Mag 2020;16:745-749


How to cite this URL:
Yoo G, Lee TH, Lee JH, Kang KB, Yang H, Park YK, Kim SY, Kim SH. Simultaneous determination of two diterpenoids, continentalic acid and kaurenoic acid, in the water extract of Aralia continentalis and their wound-healing activity. Phcog Mag [serial online] 2020 [cited 2021 Apr 17 ];16:745-749
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2020;volume=16;issue=72;spage=745;epage=749;aulast=Yoo;type=0