Pharmacognosy Magazine

: 2020  |  Volume : 16  |  Issue : 70  |  Page : 418--424

Renoprotective effects of the hydroethanolic extract of Senecio serratuloides against Nw-nitro L-arginine methyl ester-induced oxidative stress in wistar rats

Charlotte Mungho Tata1, Constance Rufaro Sewani-Rusike2, Opeoluwa Oyehan Oyedeji3, Ephraim Tobela Gwebu4, Benedicta Ngwenchi Nkeh-Chungag5,  
1 Department of Human Biology, Faculty of Health Sciences, Walter Sisulu University, Mthatha; Department of Chemical Sciences, Faculty of Science, University of Johannesburg, Johannesburg, South Africa
2 Department of Human Biology, Faculty of Health Sciences, Walter Sisulu University, Mthatha, South Africa
3 Department of Chemistry, Faculty of Science and Agriculture, University of Fort Hare, Eastern Cape Province, South Africa
4 Department of Chemistry, Faculty of Science and Technology, Rusangu University, Monze, Zambia
5 Department of Biological Sciences, Faculty of Natural Sciences, Walter Sisulu University, Mthatha, South Africa

Correspondence Address:
Benedicta Ngwenchi Nkeh-Chungag
Department of Biological Sciences, Faculty of Natural Sciences, Walter Sisulu University, Mthatha
South Africa


Background: Oxidative stress is implicated in the pathogenesis of many diseases. Proper management of oxidative stress requires antioxidants from external sources to supplement that of the body. Plants are considered as a major source of antioxidants because of their natural origin and therapeutic benefits. Objectives: This study was aimed at investigating the renoprotective and antioxidant capacity of hydroethanolic extract of Senecio serratuloides (HESS). Materials and Methods: In vitro and ex vivo antioxidant capacity of the extract was investigated. Female Wistar rats were treated with Nw-nitro L-arginine methyl ester (L-NAME) (40 mg/kg) for 4 weeks and then cotreating with L-NAME (20 mg/kg) and extract (HESS150 or HESS300 mg/kg) for 2 weeks and finally with plant extract or normal saline only for 2 weeks making a total of 8 weeks. Twenty-four-hour urine samples were collected during the study, and at the end of the study; blood and kidneys were harvested for biochemical and histological assays. Results: HESS exhibited high antiradical activity against 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) radicals with IC50values of 0.1 and 0.4 mg/ml, respectively. HESS significantly (P < 0.01) augmented L-NAME-induced decrease in creatinine clearance, glomerular filtration rate, and serum calcium concentration. HESS significantly increased in vivo antioxidant capacity (P < 0.01), decreased malondialdehyde (P < 0.01), and Bax (P < 0.001) concentration. It showed renoprotection and significantly (P < 0.01) prevented collagen deposition in the kidneys. Conclusion: S. serratuloides has renoprotective and free radical-scavenging properties and therefore maybe important in combating oxidative stress-mediated diseases in the kidneys and other parts of the body.

How to cite this article:
Tata CM, Sewani-Rusike CR, Oyedeji OO, Gwebu ET, Nkeh-Chungag BN. Renoprotective effects of the hydroethanolic extract of Senecio serratuloides against Nw-nitro L-arginine methyl ester-induced oxidative stress in wistar rats.Phcog Mag 2020;16:418-424

How to cite this URL:
Tata CM, Sewani-Rusike CR, Oyedeji OO, Gwebu ET, Nkeh-Chungag BN. Renoprotective effects of the hydroethanolic extract of Senecio serratuloides against Nw-nitro L-arginine methyl ester-induced oxidative stress in wistar rats. Phcog Mag [serial online] 2020 [cited 2022 Jul 5 ];16:418-424
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Full Text


  • This study was aimed at examining the renoprotective and antioxidant capacity of the hydroethanolic extract of Senecio serratuloides . Oxidative stress was induced in female Wistar rats using Nw-nitro L-arginine methyl ester (L-NAME), a nitric oxide synthase inhibitor. In vitro and ex vivo antioxidant capacity of the extract, as well as biochemical and histological assays of samples harvested from the rats treated with L-NAME and the plant extract, was carried out. Therefore, S. serratuloides has renoprotective and free radical-scavenging properties and therefore maybe important in combating oxidative stress-mediated diseases.


Abbreviations used: ABTS: 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid); ATPase: adenosine triphosphatase; BH4: 4-tetrahydrobiopterin; Crea: Creatinine; CPT: Captopril; cGMP: Cyclic guanosine monophosphate; DPPH: 1,1-diphenyl-2-picrylhydrazyl; ECs: Endothelial cells; eNOS: Endothelial nitric oxide synthase; FRAP: Ferric reducing antioxidant power; GAE: Gallic acid equivalent; GFR: Glomerular filtration rate; HESS: Hydroethanolic extract of Senecio serratuloides ; L-NAME (LN): Nw-nitro L-arginine methyl ester; NO: Nitric oxide; PKGI: Protein kinase GI; ROS: Reactive oxygen species; TBA: Thiobarbituric acid; TBARS: Thiobarbituric acid reactive substance; TCA: Trichloroacetic acid; TPTZ: 2,4,6-tripyridyl-s-triazine; TOC: Total antioxidant capacity; UP/UCr: Urine protein-to-urine creatinine ratio; VSMCs: Vascular smooth muscle cells.


Oxidative stress (imbalance between oxidants and antioxidants) is implicated in the pathogenesis of many diseases.[1],[2],[3] In normal physiological conditions, the production of reactive oxygen species (ROS) is tightly regulated by endogenous cellular antioxidants; hence, their rate of generation is counterbalanced by the rate of elimination. However, in pathological conditions, either due to increased ROS production or diminished antioxidant levels, the presence of ROS outweighs the usual protective antioxidant mechanisms employed by the cells, leading to a state of oxidative stress.[4] Increased production of ROS decreases nitric oxide (NO) bioavailability by direct inactivation through formation of peroxynitrite[3] or by inhibition of endothelial nitric oxide synthase (eNOS) activity through oxidation of 4-tetrahydrobiopterin (BH4) leading eNOS uncoupling.[5]

ROS also stimulate increased deposition of extracellular matrix (ECM) proteins such as collagen while modulating matrix metalloproteinases, which are responsible for degrading collagen and other ECM proteins. In addition, ROS increase intracellular free Ca2+ concentration in vascular smooth muscle cells and endothelial cells (ECs) by mobilization from reticular stores and activation of Ca2+ channels.[6],[7]

Medicinal plants are a major reservoir of phytochemicals which can play a great role in reducing occurrences of many diseases by acting as antioxidants and supplying the body with necessary nutrients.[8] Examples of these phytochemicals include tannins, saponins, phenols, terpenoids, alkaloids, and flavonoids.[9] Sterol, flavonoid, saponin, tannin, phenol, alkaloid, and cardiac glycoside have been proven to have antioxidant activities.[10],[11] These phytochemicals exhibit their antioxidant effects through; scavenging ROS or suppressing their function and upregulating or protecting antioxidant defenses[12]Senecioserratuloides is a herb which is used in traditional medicine for treating, skin disorders, sores, rashes, burns, and wounds.[13] Based on these ethnomedicinal reports, the objective of this study was to investigate the renoprotective effects of the hydroethanolic extract of S.serratuloides (HESS) by determining its antioxidant effects, its effect on renal parameters, and renal histology.

 Materials and Methods


Creatinine reagent (CR510), urea reagent (UR221), calcium reagent (CA590), potassium reagent (PT1600), and total protein reagent (UP1571) (Randox Laboratories Ltd., UK); Bradford reagent, FastCast acrylamide kit, Turbo transfer kit (170–4270), and Clarity Western ELC substrate (170–5060) (Bio-Rad Laboratories, USA); anti-Bax antibody (ab32503), anti-ß-actin antibody (ab8227), and goat anti-rabbit IgG HandL (HRP) (ab205718) (Abcam Laboratories Inc., USA); protease inhibitor (S8820-20TAB), RIPA buffer (R0278), Nω-nitro-L-arginine methyl ester, and ß-mercaptoethanol (Sigma, USA); stains (Harris hematoxylin, eosin, and picro-sirius red); and solvents (ethanol, glacial acetic acid, and methanol xylene) were of analytical grade.

Plant material and extraction

S.serratuloides whole plant was supplied by Mr Fikile Mahlakata of Lusikisiki, Eastern Cape, South Africa. It was authenticated by Dr Immelman in the KEI Herbarium of Walter Sisulu University where a voucher specimen (Tata 1/13967) was deposited. The plant was air-dried in the laboratory and pulverized and thoroughly extracted in 70% ethanol. The ethanol was recovered using a rotary evaporator (Heidolph Laborota 4000, Germany) at 35°C, and the extract was dried in an oven at the same temperature. The plant extract was then stored in a refrigerator and dissolved in distilled water before use.

Animal handling

Thirty female Wistar rats weighing 200–240 g were lodged in cages in the animal room at Walter Sisulu University which is maintained at 24°C. The room was lit by daylight and dark at night. The rats were allowed free access to rat chow and water. The ethical clearance for the study was approved the Faculty of Health Sciences Ethical Clearance Committee, Walter Sisulu University, South Africa. All animal procedures were carried out in line with the South African National Standards: The Care and Use of Animals for Scientific Purposes.[14]

Phytochemical screening

Phytochemical screening of HESS for the presence of various phytochemical constituents was done following standard procedures as described by Balamuniappan et al .[15] Total phenolic content was quantified using Folin's reagent with gallic acid as standard, whereas flavonoid content was determined using quercetin as standard.[16]

Antioxidant capacity of extract

1,1-diphenyl-2-picrylhydrazyl assay

The 1,1-diphenyl-2-picrylhydrazyl assay (DPPH) assay is a method for determining the ability of extracts to trap free radicals. The test was done following the method described by Irshad et al .[16] with modifications. DPPH radical solution was prepared in a dark room by dissolving 0.01 g of DPPH radical in 500 mL of methanol to get an absorbance of 1.5 units at 517 nm. The stock solution of the standard was prepared by dissolving 0.1 g ascorbic acid in 100 mL distilled water. 640 μL of the stock solution was added to 3360 μL distilled water to yield a concentration of 160 μg/mL working solution which was double diluted serially to several concentrations (160, 80, 40, 20, 10, 5, 2.5, and 1.25 μg/mL). The working solution of the extracts was prepared by adding 2 mL distilled water to 2 mL extract stock yielding a concentration of 5 mg/mL which was then double diluted serially to various concentrations (5, 2.5, 1.25, 0.63, 0.31, 0.16, 0.08, and 0.04 mg/mL). In a dark room, 3 mL DPPH solution was added to 1 mL of the working solutions (standard or extract) and incubation was done at room temperature for 30 min. Absorbance was measured using a spectrophotometer using a ultraviolet–visible spectrophotometer (Phoenix-2000V, UK) at 517 nm. The percentage DPPH radical-scavenging activity was calculated using the following equation:

%DPPH radical scavenging = ([Ablank− Astd/extract]/Ablank) × 100

The percentage inhibition was plotted against concentration, and IC50 values were extrapolated from plots.

2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) assay

The 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation was used to screen the radical-scavenging abilities of some phytochemicals in the crude extract. The procedure was done following the methods as described by Thaipong et al .[17] with modifications. ABTS stock solution was prepared from 7.4 mM ABTS and 2.6 mM potassium persulfate solution that is 0.4 g ABTS was dissolved in 100 mL distilled water and 0.07 g of potassium persulfate was dissolved in 100 mL distilled water. The ABTS and potassium persulfate solutions were mixed in the ratio 1:1 and incubated for 12 h in a dark room at room temperature. ABTS working solution was prepared by mixing 1 mL ABTS stock and 60 mL methanol to get an absorbance of 1.17 units at 734 nm. The working solution of the extracts was prepared by adding 1.6 mL distilled water to 0.4 mL extract stock to give a concentration of 2 mg/mL which was then double diluted serially (2, 1, 0.5, 0.25, 0.13, 0.06, and 0.03 mg/mL). The stock of the standard was prepared by dissolving 0.5 g Trolox in a few drops of dimethyl sulfoxide and making up the volume to 100 mL with ethanol. Trolox working solution was prepared by adding 1960 μl distilled water to 40 μL Trolox stock solution to give a concentration of 100 μg/mL which was double diluted serially (100, 50, 25, 12.5, 6.25, 3.13, and 1.56 μg/mL). 2850 μL ABTS working solution was added to 150 μL extract or standard and incubated for 1 h in a dark room. Absorbance was read at 734 nm using a spectrophotometer and IC50 values calculated as was done with DPPH assay.

Ferric reducing antioxidant power assay

Ferric reducing antioxidant power (FRAP) assay measures the total reducing power of electron-donating antioxidants found in a reaction mixture. It was done using method as described by Irshad et al.[16] with modifications. The stock solutions (300 mM acetate buffer [3.1 g C2H3 NaO23H2O and 16 mL glacial acetic acid (C2H4O2), 1 L distilled water], 10 mM 2,4,6-tripyridyl-s-triazine [TPTZ] solution in 40 mM HCl) was made by dissolving 0.156 g TPTZ in 50 ml HCl and then 1.1 g FeCl3 in 200 mL dH2O solution. Fresh FRAP reagent was prepared by combining 100 mL acetate buffer, 10 mL TPTZ, and 10 mL FeCl3. The standard stock was prepared by dissolving 0.1 g ascorbic acid in 100 mL distilled water to yield a concentration of 1 mg/mL. 320 μL stock solution of the standard was added to 3380 μL distilled water to give a concentration of 80 μg/ml which was double diluted serially (80, 40, 20, 10, 5, 2.5, and 1.25 μg/mL). 0.2 ml extract stock added to 0.8 mL distilled water forming a working solution of 1 mg/mL. The assay was done in triplicate; the freshly prepared FRAP reagent was added to 100 μL extract or standard and incubated in a water bath at 40°C for 4 min. Absorbance was measured at 593 nm. Results were extrapolated from standard curve and expressed in μg ascorbic acid equivalents.

Treatment with Nw-nitro L-arginine methyl ester and extract

Animals were randomly assigned to five treatment groups with six rats per group (n = 6): NT (normal saline only) group, LN (L NAME) group, CPT (captopril (20 mg/kg)) group, HESSLN150 (HESS (150 mg/kg)) group and HESSLN300 (HESS (300 mg/kg)) group.

Rats were treated with L-NAME (40 mg/kg) for 4 weeks and then cotreated with normal saline or captopril or extract and L-NAME (20 mg/kg) for 2 weeks and finally with the normal saline or captopril or extract only in the past 2 weeks.[18] Throughout the 8 weeks, the NT control group received normal saline only. Necropsy was performed at the end of the study.

Urine collection

Urine was collected in graduated cylinders by placing rats singly in metabolic cages for 24-h urine collection on day 0 before treatment and then weekly after daily oral administration of assigned treatment. Collected urine was kept in a minus 20 freezer for later analysis. The quantity of water consumed was also monitored. Urine flow was calculated using the formula:

Urine flow = 24 h urine volume/1440 ml/min

where 1440 = 24 h × 60 min.

Termination of treatment

Two days to the end of the experiment, the rats were not treated, and on the evening, they were fasted for 16 h, weighed, and sacrificed.[19] Blood was collected by cardiac puncture for serum preparation. Kidneys were harvested, and one kidney from each rat was fixed in 10% buffered formalin[20] and the other was stored in a minus 20 freezer for later analysis.

Urine analysis

Urine samples were analyzed for protein, urea, creatinine, and calcium using commercial kits (Randox Co., UK), and procedure was followed as described by manufacturer. Urine protein-to-creatinine ratio, creatinine clearance, and glomerular filtration rate (GFR) were calculated using the following equations:[21]

Urine protein-to-urine creatinine ratio (UP/UCr) = urine protein/urine creatinine

Crea clearance = (urine Crea [mg/dl] × 24 h urine [ml])/(serum Crea [mg/dl] × 1440)

GFR = (Urine Crea × Urine volume × 1000)/(serum Crea × body weight × 1440)

Where Crea: creatinine, 1440: min in 24 h, GFR: glomerular filtration rate.

Determination Bax protein concentration in kidney

Identification of Bax protein was by western blotting using commercial kits (Bio-Rad Laboratories, USA) following the method described by Singh et al .[22] with modifications. In brief, proteins in kidney homogenate were quantified using Bradford reagent. The proteins were separated by electrophoresis and blotted unto nitrocellulose membrane. The membrane was then incubated with anti-Bax antibody (ab32503), followed by goat anti-rabbit IgG HandL (HRP) (ab205718). Bound antibodies were detected by chemiluminescence using Clarity Western enhanced chemiluminescence substrate (170–5060), and imaging was done using ChemiDoc Touch Imaging System (Bio-Rad Laboratories, USA). Analysis of images was performed using Image Lab software (Bio-Rad Laboratories, USA), and Bax protein bands were normalized using housekeeping proteins (ß-actin, ab8227).

Determination of total antioxidant capacity in serum and kidney tissues

Total antioxidant capacity (TOC) of serum and tissue homogenates from treated animals was determined by FRAP assay following standard procedure[23] with modifications. Briefly, 3 ml freshly prepared FRAP reagent was added 100 μl of sample (serum, heart, or kidney homogenates), and the solutions were incubated for 15 min at room temperature. Absorbance was read at 593 nm. Antioxidant activity was extrapolated from the standard curve and expressed as ascorbic acid equivalent per milliliter of serum and per milligram of tissue.

Determination of lipid peroxidation

Thiobarbituric acid (TBA) reactive substance assay was used to evaluate lipid peroxidation. Malondialdehyde (MDA), which is an end product of polyunsaturated fatty acid peroxidation, reacted with TBA to form a pink complex, whereas trichloroacetic acid (TCA) in hydrochloric acid extracted lipids and proteins to prevent interference.[24] Briefly, 0.392 g TBA was dissolved in 75 mL of 0.25 M HCl. 5 g TCA was added to the mixture, and the volume was made up to 100 ml using 0.25 M HCl forming a TBA-TCA mixture. 500 μL of TBA-TCA mixture was then added 200 μL serum or homogenates (heart or kidney), and 150 μL PBS solutions were mixed, covered with aluminum foil, and boiled in a water bath at 100°C for 15 min. The mixtures were then allowed to cool to room temperature and centrifuged at 3000 rpm, 20°C for 10 min. 200 μL supernatant was transferred into microplate wells, and absorbance was read at 540 nm using a microplate reader (Bio-Rad model 680, USA). The concentration of MDA in micrometer was calculated using a molar extinction coefficient of 1.56 × 105/M/cm

MDA concentration (μM) = Absorbance at 540 nm × 0.156.

Renal histology

Kidney sections were fixed in 10% buffered formalin. The fixed sections were embedded in paraffin wax,[20] sectioned to 5-μm slices, and stained with hematoxylin/eosin[25] and picro-sirius red stain.[26] The sectioned slices were examined by light microscopy at ×20 and ×40, whereas semi-quantification of collagen was done using scientific image analysis software (

Statistical analysis

Statistical analysis was carried out using GraphPad Prism version 5.03 for Windows (GraphPad Software, San Diego, CA, USA). One-way analysis of variance, followed by Tukey's test for multiple comparisons, was performed to determine differences between treatment groups. Results were expressed as mean ± standard error (SEM). P < 0.05 was considered statistically significant.


Phytochemical constituents of hydroethanolic extract of Senecioserratuloides

Phytochemical analysis of HESS showed that it contains; alkaloids, phenols, steroids, tannins, saponins, flavonoids, and terpenes. The total phenolic content of HESS was 172.7 ± 1.5 μg GAE/mg of extract, whereas the total flavonoid content was found to be 36.3 ± 0.2 μg quercetin equivalent/mg of extract.

Antioxidant capacity of hydroethanolic extract of Senecioserratuloides

From the ABTS assay, HESS had an IC50 value of 0.4 mg/ml compared to the standard Trolox with IC50 of 0.07 mg/ml. HESS showed a scavenging effect on DPPH radical with an IC50 of 0.1 mg/mL compared to standard ascorbic acid of IC500.03 mg/ml. HESS equally had a strong reducing power with a value of 151 ± 0.8 μg in GAE/mg of the extract.

Effect of hydroethanolic extract of Senecioserratuloides on total antioxidant capacity

In kidney homogenates, HESS300 (44.39 ± 5 μgGAE/mg kidney tissue) had significantly (P < 0.05) higher TOC compared to the NT (27.5 ± 1 μgGAE/mg kidney tissue) and LN (28.2 ± 3 μgGAE/mg kidney tissue) control groups [Figure 1]. There was no significant difference in TOC in serum and heart samples of all treatment groups [Table S1].{Figure 1}

Effect of hydroethanolic extract of Senecioserratuloides on malondialdehyde concentration

The concentrations of MDA in serum were different among treatment groups; LN (2.29 ± 0.2 μM/ml serum; P < 0.01) and CPT (3.82 ± 0.7 μM/ml serum; P < 0.01) were significantly higher compared to the NT group (0.49 ± 0.1 μM/ml serum). On the other hand, HESS150 (0.6 ± 0.2 μM/ml serum; P < 0.05) and HESS300 (0.31 ± 0.1 μM/ml serum; P < 0.01) significantly prevented the L-NAME-induced increase in MDA concentration [Figure 2]. In heart homogenate, HESS300 (0.28 ± 0.03 μM/mg heart tissue) and CPT had significantly (P < 0.01) lower MDA concentration compared to the LN (1.22 ± 0.2 μM/mg heart tissue) group [Figure 2]. In kidney homogenate, there was no significant difference in MDA concentration although HESS300 (3.99 ± 0.1 μM/mg kidney tissue) had slightly lower MDA concentration compared to the LN (4.81 ± 0.3 μM/mg kidney tissue) group.{Figure 2}

Effect of hydroethanolic extract of Senecioserratuloides renal function

Results from renal function tests showed that L-NAME significantly increased urine output and hence urine flow; it decreased creatinine, urea, and calcium excretion in urine and induced proteinuria with higher protein-to-creatinine ratios in the LN group compared to the NT group. HESS150 and HESS300 significantly decreased urine output, increased urea, and creatinine excretion, whereas HESS300 increased calcium excretion and decreased proteinuria compared to the LN control group [Table 1].{Table 1}

In serum, L-NAME significantly increased calcium and creatinine concentrations in the LN group compared to the NT group. It was also observed that creatinine clearance and GFR in the LN group were lower compared to all treatment groups; meanwhile, HESS150 and HESS300 significantly increased creatinine clearance and GFR compared to the LN control group [Table 2]. There was, however, no significant difference in blood urea nitrogen and potassium concentration between the treatment groups in serum.{Table 2}

Effect of hydroethanolic extract of Senecioserratuloides on Bax concentration in the kidney

[Figure 3] shows blots of Bax and graph with normalized concentrations of Bax in kidneys. L-NAME significantly (P < 0.05) increased the expression of Bax in the kidney compared to the NT control. HESS150, HESS300, and CPT had significantly (P < 0.001) lower Bax concentration compared to the LN control group.{Figure 3}

Effect of hydroethanolic extract of Senecioserratuloides on renal tissue

L-NAME caused glomerular degeneration and shrinkage that was observed with both stains. HESS prevented these degeneration and shrinkages in a dose-dependent manner. There was a significant deposition of collagen in kidney samples from the LN group (0.84 ± 0.1%) compared to the NT group (0.15 ± 0.02%). This L-NAME-induced fibrosis was significantly (P < 0.01) attenuated by HESS150 (0.31 ± 0.03%), CPT (0.15 ± 0.03%), and HESS300 (0.27 ± 0.03%) [Figure 4].{Figure 4}


Results from this study showed that HESS exhibited high antioxidant capacity which was evident in its ability to improve TOC and prevent lipid peroxidation.In vivo HESS prevented L-NAME-induced proteinuria, increased creatinine clearance, GFR, and serum calcium concentration while decreasing the concentration of Bax proteins in kidneys and protecting the kidneys.

The high antioxidant capacity of HESS was due to the presence of a wide range of phytochemicals such as polyphenols (phenols, flavonoids, tannins, and lignins) and terpenoids. Oxidative stress may hasten renal injury development through cytotoxicity[27] as seen with increase in both plasma and renal MDA levels, which suggests that the levels of ROS in plasma could reflect its production in the kidney. The antioxidants found in HESS could have prevented oxidative stress by either suppressing ROS formation, scavenging of ROS, or by upregulating or protecting antioxidant defenses.[12] Thein vitro antioxidant capacity of HESS was translatedin vivo as it showed higher TOC and prevented lipid peroxidation.

The protective role of HESS and CPT against oxidative stress may have been through scavenging of free radicals or preventing of free radical formation. Captopril has the ability to scavenge free radicals due to the presence of a sulfhydryl group; thus, it is capable of preventing oxidant-induced cell injury.[28] HESS may also have been capable of preventing oxidation of NO to peroxynitrite or oxidation of BH4 by ROS, thus preventing eNOS uncoupling and oxidative stress.

The HESS extract was found to prevent proteinuria and increase creatinine clearance, urea excretion, and GFR. The function of HESS in renal protection was further reinforced by its ability to prevent glomerular degeneration/shrinkage which is generally observed with L-NAME treatment. Oxidative stress has a role in the induction of cardiac dysfunction in renal patients.[29] In fact, cardiovascular morbidity and mortality occurs throughout renal dysfunction progression even in patients with moderate renal insufficiency,[27] and most of the patients with renal dysfunction die of cardiovascular reasons rather than progression to complicated renal disease.[27] Given that HESS had the ability to prevent oxidative stress in the heart and kidneys and previous studies have reported its ability to prevent cardiac hypertrophy,[30] it suggests that it may be important in combating renal dysfunction as well as associated cardiovascular disorders.

It also prevented L-NAME-induced release of Bax in the kidney. The renoprotective effect of HESS could also have been through inhibiting inflammation and oxidative stress. Increased glomerular capillary wall pressure and oxidative stress may have accounted for the elevated levels of Bax and hence apoptosis revealed by glomerular degeneration. Studies done in some cell lines have proposed the cytoprotective role of NO through inactivation of caspase-3 through S-nitrosylation; thus, decreased NO production in L-NAME model can promote apoptosis.[31]

The decreased concentration of calcium in serum induced by L-NAME was prevented by HESS. This suggested that the protective role of HESS may have been by preventing calcium influx through its upregulation of NO availability. Iyú et al .[32] investigated calcium signaling in platelets from L-NAME-treated rats. They found that reduction of NO altered the regulation of platelet calcium levels such that calcium entry from the extracellular space and mobilization from the internal stores were enhanced.[32] This can be explained by the fact that NO is an important regulator of intracellular calcium level, and it activates soluble guanylyl cyclase which leads to increase cyclic guanosine monophosphate (cGMP). cGMP inhibits voltage-gated calcium channels and activates protein kinase GI which phosphorylates calcium-dependent potassium channels, inositol trisphosphate receptor-associated cGMP kinase substrate, and sarcoplasmic reticulum adenosine triphosphatase.[33] Thus resulting in the sequestration of calcium in the sarcoplasmic reticulum and thereby decreasing intracellular calcium flux.[34] NO also directly inhibits calcium-dependent potassium channels preventing calcium influx.[33] This mechanism supported the proposal that L-NAME-induced decrease in serum calcium may be due to increased calcium influx due to decreased bioavailability of NO.


The HESS had free radical-scavenging properties and hence renoprotective properties. Therefore, it may serve as a potential source of antioxidants which can be used for treating oxidative stress-induced diseases.

Financial support and sponsorship

This work was supported by the National Research Foundation (NRF Grant UID 93177), South Africa, and the National Institute of Minority Health and Health Disparities/National Institutes of Health (Grant # 5T37MD001810).

Conflicts of interest

There are no conflicts of interest.


1Araujo M, Wilcox CS. Oxidative stress in hypertension: Role of the kidney. Antioxid Redox Signal 2014;20:74-101.
2Montezano AC, Touyz RM. Oxidative stress, Noxs, and hypertension: Experimental evidence and clinical controversies. Ann Med 2012;44 Suppl 1:S2-16.
3González J, Valls N, Brito R, Rodrigo R. Essential hypertension and oxidative stress: New insights. World J Cardiol 2014;6:353-66.
4Virdis A, Duranti E, Taddei S. Oxidative stress and vascular damage in hypertension: Role of angiotensin II. Int J Hypertens 2011;2011:916310.
5Dinh QN, Drummond GR, Sobey CG, Chrissobolis S. Roles of inflammation, oxidative stress, and vascular dysfunction in hypertension. Biomed Res Int 2014;2014:406960.
6Schächinger V, Zeiher AM. Atherogenesis–recent insights into basic mechanisms and their clinical impact. Nephrol Dial Transplant 2002;17:2055-64.
7Xu S, Touyz RM. Reactive oxygen species and vascular remodelling in hypertension: Still alive. Can J Cardiol 2006;22:947-51.
8Ntie-Kang F, Lifongo LL, Mbaze LM, Ekwelle N, Owono Owono LC, Megnassan E, et al . Cameroonian medicinal plants: A bioactivity versus ethnobotanical survey and chemotaxonomic classification. BMC Complement Altern Med 2013;13:147.
9Konate K, Yomalan K, Sytar O, Zerbo P, Brestic M, Patrick VD, et al . Free radicals scavenging capacity, antidiabetic and antihypertensive activities of flavonoid-rich fractions from leaves of trichilia emetica and opilia amentacea in an animal model of type 2 diabetes mellitus. Evid Based Complement Alternat Med 2014;2014:867075.
10Li Y, Adachi T, Bolotina VM, Knowles C, Ault KA, Cohen RA. Abnormal platelet function and calcium handling in Dahl salt-hypertensive rats. Hypertens (Dallas, Tex 1979) 2001;37:1129-35.
11Tapas AR, Sakarkar DM, Kakde RB. Flavonoids as nutraceuticals: A review. 2008;7:1089-99.
12Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: An overview. ScientificWorldJournal 2013;2013:162750.
13De Wet H, Nciki S, van Vuuren SF. Medicinal plants used for the treatment of various skin disorders by a rural community in Northern Maputaland, South Africa. J Ethnobiol Ethnomed 2013;9:51.
14South African National Standards. SANS 10386:2008-The Care and Use of Animals for Scientific Purposes. SABS Stand Div; 2008. p. 1-43.
15Balamuniappan G, Anandalakshmi R, Geetha S, Kumar NS, Durai M. Qualitative and quantitative analysis of phytochemicals in crude extract of big – Leaf mahogany (Swietenia macrophylla King.). Int J Herb Med IJHM 2016;88:88-91.
16Irshad M, Zafaryab M, Singh M, Rizvi MM. Comparative analysis of the antioxidant activity of Cassia fistula extracts. Int J Med Chem 2012;2012:1-6.
17Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Hawkins Byrne D. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compos Anal 2006;19:669-75.
18Nina C, Lane J, All A, Reserved R. Effects of Heme-l-Arginate on l-Name Induced Hypertension A Thesis Submitted to The College of Graduate Studies & Research in Partial Fulfillment of the Requirements for the Degree of Master of Science in the Department of Physiology Nina Jean Lane; 2012.
19Chandran G, Sirajudeen KN, Nik Yusoff NS, Swamy M, Samarendra MS. Effect of the antihypertensive drug enalapril on oxidative stress markers and antioxidant enzymes in kidney of spontaneously hypertensive rat. Oxid Med Cell Longev 2014;2014:1-10.
20Perry C, Chung JY, Ylaya K, Choi CH, Simpson A, Matsumoto KT, et al . A buffered alcohol-based fixative for histomorphologic and molecular applications. J Histochem Cytochem 2016;64:425-40.
21Bazzano T, Restel TI, Porfirio LC, de Souza AS, Silva IS. Renal biomarkers of male and female Wistar rats (Rattus norvegicus ) undergoing renal ischemia and reperfusion. Acta Cir Bras 2015;30:277-88.
22Singh L, Pushker N, Saini N, Sen S, Sharma A, Bakhshi S, et al . Expression of pro-apoptotic Bax and anti-apoptotic Bcl-2 proteins in human retinoblastoma. Clin Experiment Ophthalmol 2015;43:259-67.
23Saeed N, Khan MR, Shabbir M. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complement Altern Med 2012;12:221.
24Olszewska-Słonina DM, Mątewski D, Czajkowski R, Olszewski KJ, WoŸniak A, Odrowąż-Sypniewska G, et al . The concentration of thiobarbituric acid reactive substances (TBARS) and paraoxonase activity in blood of patients with osteoarthrosis after endoprosthesis implantation. Med Sci Monit 2011;17:CR498-504.
25Fischer AH, Jacobson KA, Rose J, Zeller R. Hematoxylin and eosin staining of tissue and cell sections. CSH Protoc 2008;2008:pdb.prot4986.
26Kiernan JA, Histological and Histochemical Methods: Theory and Practice, Ed. 3 Butterworth Heinemann, Oxford, UK; 1999.
27Cachofeiro V, Goicochea M, De Vinuesa SG, Oubĩa P, Lahera V, Lũo J. Oxidative stress and inflammation, a link between chronic kidney disease and cardiovascular disease. Kidney Int 2008;74 (Suppl 111):S4-9.
28Kojšová S, Jendeková L, Zicha J, Kuneš J, Andriantsitohaina R, Pecháňová O, et al . The effect of different antioxidants on nitric oxide production in hypertensive rats. Physiol Res 2006;55 Suppl 1:3-16.
29Nuhu F, Bhandari S. Oxidative stress and cardiovascular complications in chronic kidney disease, the impact of anaemia. Pharmaceuticals 2018;11:1-15.
30Tata CM, Sewani-Rusike CR, Oyedeji OO, Gwebu ET, Mahlakata F, Nkeh-Chungag BN. Antihypertensive effects of the hydro-ethanol extract of Senecio serratuloides DC in rats. BMC Complement Altern Med 2019;19:1-10.
31Kim YM, Kim JH, Kwon HM, Lee DH, Won MH, Kwon YG, et al . Korean Red Ginseng protects endothelial cells from serum-deprived apoptosis by regulating Bcl-2 family protein dynamics and caspase S-nitrosylation. J Ginseng Res 2013;37:413-24.
32Iyú D, Atucha NM, Martínez-Prieto C, Ortiz MC, García-Estañ J. Altered calcium signaling in platelets from nitric oxide-deficient hypertensive rats. Cell Commun Signal 2004;2:1.
33Walford G, Loscalzo J. Nitric oxide in vascular biology. J Thromb Haemost 2003;1:2112-8.
34Nossaman B, Pankey E, Kadowitz P. Stimulators and activators of soluble guanylate cyclase: Review and potential therapeutic indications. Crit Care Res Pract 2012;2012:290805.