Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 16  |  Issue : 67  |  Page : 68--75

Extraction, purification, content analysis and hypoglycemic effect of mulberry marc anthocyanin


Ji-Li Fang1, Shan-Shan Jia1, Yin Lin1, Ke Yuan2, Song-Heng Jin4 
1 Zhejiang Agriculture and Forestry University, Lin'an, P.R. China
2 Zhejiang Agriculture and Forestry University, Lin'an; Jiyang College of Zhejiang Agriculture and Forestry University, Zhu'ji, P.R. China

Correspondence Address:
Ke Yuan
Jiyang College of Zhejiang Agriculture and Forestry University, Zhuji 311800
P.R. China
Song-Heng Jin
Jiyang College of Zhejiang Agricultureand Forestry University, Zhuji 311800
P.R. China

Background: Mulberry marc (MM) which as a wasted residue contains many available resources and one of the valuable nutrients is anthocyanin. In this study, the extraction, purification, content analysis, and hypoglycemic effect of anthocyanin from MM were studied. Materials and Methods: The total anthocyanin and cyanidin-3-O-glucoside of the purified anthocyanin of mulberry marc (MMA) were determined using pH differential method and high-performance liquid chromatography. The hypoglycemic effects were studied by streptozotocin and high-fat diet-induced type 2 diabetic rats. The rats were given MMA for 4 weeks and at the end of experiment; the blood was collected for determination serum indexes; the tissue of liver, kidney, pancreas, and spleen was removed for weighting and observing pathological changes. Results: The content of total anthocyanin and cyanidin-3-O-glucoside of MMA was 39.72% and 18.63%, respectively. The fasting blood glucose of rats in the MMA treatment groups was decreased. The lipid metabolites (triglycerides and total cholesterol) and malondialdehyde were decreased and superoxide dismutase was increased of type 2 diabetes rats after MMA treated; the pathological changes were alleviated in various degrees. Conclusion: The content of anthocyanin in mulberry marc is higher than mulberry juice, and the MMA has a good hypoglycemic effect.


How to cite this article:
Fang JL, Jia SS, Lin Y, Yuan K, Jin SH. Extraction, purification, content analysis and hypoglycemic effect of mulberry marc anthocyanin.Phcog Mag 2020;16:68-75


How to cite this URL:
Fang JL, Jia SS, Lin Y, Yuan K, Jin SH. Extraction, purification, content analysis and hypoglycemic effect of mulberry marc anthocyanin. Phcog Mag [serial online] 2020 [cited 2021 Apr 18 ];16:68-75
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2020;volume=16;issue=67;spage=68;epage=75;aulast=Fang;type=0