Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 16  |  Issue : 67  |  Page : 192--196

Red seaweed (Eucheuma cottonii) extract promotes human keratinocyte migration via p38 mitogen-activated protein kinase phosphorylation


In Wook Kim1, So Yeon Kim1, Marianti Manggau2, Harfina Finanda Anwar1, Nyoun Soo Kwon1, Kwang Jin Baek1, Hye-Young Yun1, Dong-Seok Kim1 
1 Department of Biochemistry, Chung-Ang University College of Medicine, Dongjak-gu, Seoul, Korea
2 Faculty of Pharmacy, Hasanuddin University, Makassar, Indonesia

Correspondence Address:
Dong-Seok Kim
Department of Biochemistry,Chung-Ang University College of Medicine, 84 Heukseok-ro, Dongjak-gu, Seoul 06974
Korea

Background: Eucheuma cottonii (EC) is a rapidly growing red seaweed in Southeast Asia. Objectives: This study was performed to investigate the effects of EC extract on human keratinocyte migration. Materials and Methods: Cell migration was assessed using the scratch wound assay. Western blot analysis was performed to investigate the EC extract-induced signaling pathways. Results: EC extract promoted HaCaT keratinocyte migration in a concentration-dependent manner. To investigate the mechanism of EC extract-induced migration, we examined the migration-related signaling pathways. Western blot analysis showed that the EC extract showed no changes in extracellular signal-activated kinase (ERK) but showed slight Akt activation. In contrast, the EC extract strongly phosphorylated p38 mitogen-activated protein kinase (MAPK) and the subsequent downstream molecule, cAMP response element-binding protein (CREB). To examine the involvement of p38 MAPK pathway in EC extract-induced migration, SB203580, a specific inhibitor of p38 MAPK, was used. The results showed that EC extract-induced migration was abrogated by SB203580 pretreatment. In addition, SB203580 also blocked p38 MAPK and CREB activation. Conclusion: Taken together, these data suggest that EC extract promotes migration in human keratinocytes via p38 MAPK and CREB phosphorylation.


How to cite this article:
Kim IW, Kim SY, Manggau M, Anwar HF, Kwon NS, Baek KJ, Yun HY, Kim DS. Red seaweed (Eucheuma cottonii) extract promotes human keratinocyte migration via p38 mitogen-activated protein kinase phosphorylation.Phcog Mag 2020;16:192-196


How to cite this URL:
Kim IW, Kim SY, Manggau M, Anwar HF, Kwon NS, Baek KJ, Yun HY, Kim DS. Red seaweed (Eucheuma cottonii) extract promotes human keratinocyte migration via p38 mitogen-activated protein kinase phosphorylation. Phcog Mag [serial online] 2020 [cited 2021 Apr 17 ];16:192-196
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2020;volume=16;issue=67;spage=192;epage=196;aulast=Kim;type=0