Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 15  |  Issue : 66  |  Page : 538--544

SUNCA standardized extract from Helianthus annus L exerts enhanced digestive enzyme activity and subsides obesity through inhibition of C/EBP-α and PPAR-γ expression both in in vitro and in vivo model


K Gouthamchandra1, HV Sudeep1, Amritharaj1, HB Lingaraju2, K Shyam Prasad3 
1 Department of Biomedicinal Research, R&D Centre for Excellence, Vidya Herbs Pvt. Ltd, Bangalore, Karnataka, India
2 Phytochemistry, R&D Centre for Excellence, Vidya Herbs Pvt. Ltd, Bangalore, Karnataka, India
3 Department of Biomedicinal Research; Phytochemistry, R&D Centre for Excellence, Vidya Herbs Pvt. Ltd, Bangalore, Karnataka, India

Correspondence Address:
K Gouthamchandra
Department of Biomedicinal Research, R&D Centre for Excellence, Vidya Herbs Pvt. Ltd, #14A, Jigani I Phase, Bengaluru - 560 105, Karnataka
India

Background: Helianthus annuus L. (sunflower) is well known for its edible oil, sunflower seed has been used as folk remedy for several diseases including cancer. Materials and Methods: In this study, SUNCA, a standardized extract enriched with chlorogenic acid (CGA) from sunflower seed was investigated for in vitro antidiabetic activity using α-amylase and α-glucosidase enzyme inhibition assay. Further, for preventing obesity, we demonstrated anti-adipogenesis in 3T3-L1 cell culture system and on body weight gain and adiposity in rat fed high-fat diet (HFD). Results:SUNCA showed significant inhibitory activity toward digestive enzymes such as α-amylase and α-glucosidase. On the other hand, SUNCA significantly inhibits lipid accumulation during adipogenesis in a dose-dependent manner. The treatment with 100 μg/kg b.w of SUNCA apparently prevented the body weight gain and the increase of triglyceride and total cholesterol level in rat fed an HFD for 6 weeks. Further immunoblotting studies reveal that SUNCA significantly down-regulates the expression of transcription factors peroxisome proliferator-activated receptor gamma and CCAT/enhancer-binding protein alpha, both in adipocytes and adipose tissues. Conclusion: The findings reported here provide the therapeutic potential of SUNCA, a standardized extract from Helanthus annus L.


How to cite this article:
Gouthamchandra K, Sudeep H V, Amritharaj, Lingaraju H B, Prasad K S. SUNCA standardized extract from Helianthus annus L exerts enhanced digestive enzyme activity and subsides obesity through inhibition of C/EBP-α and PPAR-γ expression both in in vitro and in vivo model.Phcog Mag 2019;15:538-544


How to cite this URL:
Gouthamchandra K, Sudeep H V, Amritharaj, Lingaraju H B, Prasad K S. SUNCA standardized extract from Helianthus annus L exerts enhanced digestive enzyme activity and subsides obesity through inhibition of C/EBP-α and PPAR-γ expression both in in vitro and in vivo model. Phcog Mag [serial online] 2019 [cited 2021 Apr 19 ];15:538-544
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2019;volume=15;issue=66;spage=538;epage=544;aulast=Gouthamchandra;type=0