Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 15  |  Issue : 62  |  Page : 47--53

Cytoprotective effect of Cactus cladode (Opuntia ficus-indica) against chlorpyrifos induced reactive oxygen species in rat hepatocytes: Involvement of heat shock protein 70 and CYP1A1/2 proteins


Hafiz Antar Makeen1, Saida Ncibi2, Syam Mohan3, Abdullah Farasani3, Roger Rahmani4, Mohammed Al Bratty5, Hassan A Alhazmi5 
1 Department of Clinical Pharmacy, Pharmacy Practice Research Unit, Jazan, Kingdom of Saudi Arabia
2 Research Unit BMG, Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gafsa, Zarroug, Gafsa, Tunisia; Department of Biology, Faculty of Science, Jazan University, Jazan, Kingdom of Saudi Arabia
3 Medical Research Centre, Jazan University, Jazan, Kingdom of Saudi Arabia
4 Toxicologie Cellulaire et Molecularie des Xenobiotiques, Sophia Antipolis, France
5 Department of Pharmaceutical Chemistry, College of Pharmacy, Jazan University, Jazan, Kingdom of Saudi Arabia

Correspondence Address:
Syam Mohan
Medical Research Center, Jazan University, Jazan
Kingdom of Saudi Arabia

Background: Organophosphorus insecticides are well known to induce hepatotoxicity. One among this is chlorpyrifos (CPF), which is an insecticide inducing various toxicities including in liver. Objective: This investigation focused on CPF-induced oxidative damage in rat hepatocytes primary culture and the protective effect of Cactus cladode aqueous extract. Materials and Methods: Hepatocytes were treated with CPF (50, 75, and 150 μM) and cactus aqueous extract. On treatment for 48 h, mortality within these cells was determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide test, reactive oxygen species (ROS) levels were measured by H2DCFDA test. Furthermore, heat shock protein 70 (HSP70) and CYP1A1/2 levels were determined using western blot analysis. Annexin V and 4',6-diamidino-2-phenylindole analysis was run to determine the level of cell death and cytoprotection exerted by CPF and extracts, respectively. Results: The results showed that CPF increases the levels of H2O2 and HSP70 and induces CYP1A1/2 and mortality within these cells. In the other side of this study, the co-treatment of these cells with CPF and Cactus cladode aqueous extract showed a recovery of these parameters. It also has been found that the Cactus cladode aqueous extract has the potential to do cytoprotective effect by preventing necrosis induced by CPF. Conclusion: Taken together, these findings suggest that the toxicity exerted by CPF in hepatocytes are involved with the generation of ROS and the regulation of well-controlled programmed cell death, which could be well protected by the Cactus cladode extract pretreatment.


How to cite this article:
Makeen HA, Ncibi S, Mohan S, Farasani A, Rahmani R, Al Bratty M, Alhazmi HA. Cytoprotective effect of Cactus cladode (Opuntia ficus-indica) against chlorpyrifos induced reactive oxygen species in rat hepatocytes: Involvement of heat shock protein 70 and CYP1A1/2 proteins.Phcog Mag 2019;15:47-53


How to cite this URL:
Makeen HA, Ncibi S, Mohan S, Farasani A, Rahmani R, Al Bratty M, Alhazmi HA. Cytoprotective effect of Cactus cladode (Opuntia ficus-indica) against chlorpyrifos induced reactive oxygen species in rat hepatocytes: Involvement of heat shock protein 70 and CYP1A1/2 proteins. Phcog Mag [serial online] 2019 [cited 2021 Jun 12 ];15:47-53
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2019;volume=15;issue=62;spage=47;epage=53;aulast=Makeen;type=0