Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 15  |  Issue : 62  |  Page : 30--33

In vitro evaluation of the effects of lycopene on caspase system and oxidative DNA damage in high-glucose condition


Bunyamin Bazyel1, Semiha Dede2, Sedat Cetin2, Veysel Yuksek3, Mehmet Taşpinar4 
1 Health Science High School, Mus Alparslan University, Mus, Turkey
2 Department of Biochemistry, Veterinary Medical Faculty, Van Yuzuncu Yil University, Van, Turkey
3 Ozalp Vocational High School, Van Yuzuncu Yil University, Van, Turkey
4 Department of Medical Biology, Faculty of Medicine, Van Yuzuncu Yil University, Van, Turkey

Correspondence Address:
Semiha Dede
Department of Biochemistry, Veterinary Medical Faculty, Van Yuzuncu Yil University, Van
Turkey

Aim and Background: The present study was planned to investigate the effects of lycopene, on the caspase-dependent apoptosis in high-dose glucose (HG)-treated PC12 cell line. PC12 cells were cultured in vitro. Materials and Methods: HG was prepared as G (250 mM), and lycopene was prepared as L1 (10 μM), L2 (20 μM), and L3 (40 μM). After 6 h of incubation, the cells were exposed to trypsin, and the samples were obtained with freeze/thaw method. Caspase 3, 8, 9; 8-hydroxy-2-deoxyguanosine (8-OHdG); and M30 were determined (enzyme-linked immunosorbent assay). Results: 8-OHdG increased in L3 (P ≤ 0.001), whereas L1 caused a decrease in HG group (P ≤ 0.001). Caspase-3 decreased significantly in L1, L2, and L3G compared to control (P ≤ 0.001) group. Caspase-8 increased significantly in L1, L1G, L2G, and all L3 glucose groups (P ≤ 0.001). There was no difference for Caspase-9. M30 was not affected by L and HG, which decreased significantly (P ≤ 0.001). Conclusion: As a result, it was determined that, when PC12 cell line was treated with HG, lycopene application had effects on caspase enzymes and DNA damage.


How to cite this article:
Bazyel B, Dede S, Cetin S, Yuksek V, Taşpinar M. In vitro evaluation of the effects of lycopene on caspase system and oxidative DNA damage in high-glucose condition.Phcog Mag 2019;15:30-33


How to cite this URL:
Bazyel B, Dede S, Cetin S, Yuksek V, Taşpinar M. In vitro evaluation of the effects of lycopene on caspase system and oxidative DNA damage in high-glucose condition. Phcog Mag [serial online] 2019 [cited 2021 Jun 25 ];15:30-33
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2019;volume=15;issue=62;spage=30;epage=33;aulast=Bazyel;type=0