Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 15  |  Issue : 61  |  Page : 232--237

Biological evaluation and molecular docking study of metabolites from Salvadora Persica L. Growing in Egypt


Mohammed M Ghoneim1, Wael M Afifi3, Mohamed Ibrahim2, Mohamed Elagawany3, Maan T Khayat4, Mohamed H Aboutaleb5, Ahmed M Metwaly7 
1 Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt; Department of Natural Products, School of Pharmacy, Almaarefa Colleges for Science and Technology, Riyadh, Saudia Arabia
2 Department of Organic Chemistry, College of Pharmacy, Misr University for Science and Technology, Al-Motamayez District, Damnhour, Egypt
3 Department of Pharmaceutical Organic Chemistry, College of Pharmacy, Damnhour University, Damnhour, Egypt
4 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudia Arabia
5 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Horus University, New Damietta 34517, Egypt

Correspondence Address:
Ahmed M Metwaly
Department of Pharmacognosy, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo 11884
Egypt

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a resistant staph bacterium to several antibiotics causing several lives-threating diseases such as pneumonia and sepsis. Meswak, Salvadora persica, exhibited promising antimicrobial properties before. Objective: Exploring the anti-MRSA activity of S. persica L. metabolites and its mechanism of action on a molecular level. Materials and Methods: Structure elucidation of the isolated metabolites was carried out by spectroscopic data (one-dimensional and two-dimensional nuclear magnetic resonance). The biological activities of the isolated metabolites against MRSA were evaluated and the molecular mode of action against the dehydrosqualene synthase enzyme have been done. Results: Four compounds have been isolated and identifies to be; apigenin (1), luteolin (2), astragalin (3), and kaempferol-3-O-rhamnoside (4). Compounds 1–4 showed good anti-MRSA activities with IC50 values of 10.3, 11.5, 3.5, and 4.5 μg/mL, respectively. In consistent, astragalin and kaempferol-3 rhamnoside showed close high docking scores. Herein, we are reporting the molecular determinates of activity of these new scaffolds as anti-MRSA, which would be of great importance to developing new anti-MRSA candidates. Abbreviations Used: 1D: One-dimensional; 2D: Two-dimensional; CC: Column chromatography; COSY: Correlations spectroscopy; DMSO: Dimethyl sulfoxide; HMBC: Heteronuclear multiple-bond correlation experiment; HRESIMS: High-resolution electrospray ionization mass spectrometry; HSQC: Heteronuclear single-quantum correlation; IR: Infrared; MRSA: Methicillin-resistant Staphylococcus aureus; NMR: Nuclear magnetic resonance; RP: Reversed phase; TLC: Thin-layer chromatography; UV: Ultraviolet; VLC: Vacuum liquid chromatography.


How to cite this article:
Ghoneim MM, Afifi WM, Ibrahim M, Elagawany M, Khayat MT, Aboutaleb MH, Metwaly AM. Biological evaluation and molecular docking study of metabolites from Salvadora Persica L. Growing in Egypt.Phcog Mag 2019;15:232-237


How to cite this URL:
Ghoneim MM, Afifi WM, Ibrahim M, Elagawany M, Khayat MT, Aboutaleb MH, Metwaly AM. Biological evaluation and molecular docking study of metabolites from Salvadora Persica L. Growing in Egypt. Phcog Mag [serial online] 2019 [cited 2021 Sep 25 ];15:232-237
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2019;volume=15;issue=61;spage=232;epage=237;aulast=Ghoneim;type=0