Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 15  |  Issue : 60  |  Page : 29--35

Comparative research of the curative effects of pinelliae rhizoma and pinelliae rhizoma praeparatum cum alumine on ovalbumin-induced allergic asthma in rats


Wei Peng, Da-Neng Wei, Yu-Jie Liu, Meng-Meng Zhang, Mei-Bian Hu, Chun-Jie Wu 
 Department of Chinese Medicinal Processing, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China

Correspondence Address:
Chun-Jie Wu
School of Pharmacy, Chengdu University of Traditional Chinese Medicine, No. 1166 Liutai Avenue, Chengdu 610075
China

Aim: This study was designed to compare the curative effects of Pinelliae Rhizoma (RPT) and Pinelliae Rhizoma Praeparatum cum Alumine (QPT) on ovalbumin (OVA)-induced allergic asthma in rats. Materials and Methods: Asthmatic rats were prepared by intraperitoneal injection and inhalation of OVA, and RPT and QPT at the doses of 120 and 240 mg/kg/day were administered orally for consecutive 7 days. Then, histopathological changes of the lung tissues were examined; levels of interleukin (IL)-4 and interferon (IFN)-γ in the bronchoalveolar lavage fluids (BALFs) and immunoglobulin (Ig) E in the serum were measured by enzyme-linked immunosorbent assay; eosinophils (EOS) in BALFs were also determined. Furthermore, the expressions of mucin 5AC (MUC5AC) and AQP-5 in the lung tissues were determined by quantitative real-time fluorogenic polymerase chain reaction and western blot assays. Besides, high-performance liquid chromatography-LTQ-Orbitrap-Elite-MS analysis was applied to compare the constituent's difference between the RPT and QPT. Results: The present results revealed that both the RPT and QPT have the potential curative effects for treating OVA-induced asthma in rats via inhibiting inflammatory reactions (suppressing EOS influx, IgE secretion, and release of IL-4 whereas increasing the release of IFN-γ) and modulating the mRNA and protein expressions of MUC5AC and AQP-5. In addition, our results also evidenced that QPT exhibited a better curative effect on allergic asthmatic rats compared to RPT. Conclusion: Our results are beneficial for confirming the traditional Chinese medicinal theory of “QPT is good at removing dampness to reduce phlegm.” Abbreviations used: BALF: Bronchoalveolar lavage fluid; ELISA: Enzyme-linked immunosorbent assay; EOS: Eosinophils; IFN: Interferon; IL: Interleukin; OVA: Ovalbumin; qRT-PCR: Quantitative real-time fluorogenic polymerase chain reaction; QPT: Rhizoma Pinelliae Praeparatum cum Alumine; RIPA: Radioimmunoprecipitation assay; RPT: Pinelliae Rhizoma; SDS-PAGE: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis; TCM: Traditional Chinese medicine.


How to cite this article:
Peng W, Wei DN, Liu YJ, Zhang MM, Hu MB, Wu CJ. Comparative research of the curative effects of pinelliae rhizoma and pinelliae rhizoma praeparatum cum alumine on ovalbumin-induced allergic asthma in rats.Phcog Mag 2019;15:29-35


How to cite this URL:
Peng W, Wei DN, Liu YJ, Zhang MM, Hu MB, Wu CJ. Comparative research of the curative effects of pinelliae rhizoma and pinelliae rhizoma praeparatum cum alumine on ovalbumin-induced allergic asthma in rats. Phcog Mag [serial online] 2019 [cited 2022 Oct 1 ];15:29-35
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2019;volume=15;issue=60;spage=29;epage=35;aulast=Peng;type=0