Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 14  |  Issue : 58  |  Page : 597--603

Ultra-high performance liquid chromatography/triple quadrupole mass spectrometry study of the stabilities and transformations of four alisols in Alismatis Rhizoma and proprietary traditional Chinese medicine prescriptions


Chun-Xin Xiao1, Fan He2, Miao-Ting Yang3, Fan Xia4, Wai-Jiao Tang3, Ben-Jie Zhou5, Hua Zhou6 
1 Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou; Faculty of Chinese Medicine, Macau University of Science and Technology, Taipa, Macao, China
2 State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao, China
3 Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou, China
4 Department of Pharmacy, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China
5 Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou; Department of Pharmacy, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China
6 Faculty of Chinese Medicine; State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao, China

Correspondence Address:
Ben-Jie Zhou
Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou 510282. Department of Pharmacy, The Seventh Affiliated Hospital, Sun Yat-Sen University, Shenzhen 518017
China
Hua Zhou
Faculty of Chinese Medicine; State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao
China

Background: Alismatis Rhizoma (AR) is a traditional Chinese medicine that is frequently used for the treatment of hyperlipidemia, hypertension, diabetes, and chronic kidney failure. Active alisols contribute to the beneficial effects of AR and AR products. Objective: In the present study, we investigated the stabilities and transformations of alisol B 23-acetate (B23), alisol A 24-acetate (A24), alisol A (AA), and alisol B (AB) in AR and proprietary traditional Chinese medicine prescriptions. Materials and Methods: We first established an ultra-high performance liquid chromatography/triple quadrupole mass spectrometry method for the analysis of AR products. Next, extracts and concentrates of AR and the proprietary prescriptions were prepared and heated at different temperatures. Finally, the developed method was applied to the analysis of these samples. Results: Our method is sensitive, precise, accurate, and reliable for the analysis of these four compounds. The extraction efficacies of the alisols from decoctions prepared from single herb and from mixtures of herbs showed large differences (P < 0.01). The alisols are highly sensitive to changes in temperature and pH and transform during heating. B23 can be transformed to AA and A24, and AB can be transformed to AA. Conclusion: These results indicate that the content change of aliols may affect the quality and efficacy of AR and AR products. Therefore, temperature and pH should be controlled during the manufacture of AR and AR products. Abbreviations used: AR: Alismatis Rhizoma; P1: Prescription 1; HGQZ: Hugan Qingzhi formula; P2: Prescription 2; AA: Alisol A; A24: Alisol A 24-acetate; AB: Alisol B; B23: Alisol B 23-acetate; UHPLC–QqQ–MS/MS: Ultra-high performance liquid chromatography/triple quadrupole mass spectrometry.


How to cite this article:
Xiao CX, He F, Yang MT, Xia F, Tang WJ, Zhou BJ, Zhou H. Ultra-high performance liquid chromatography/triple quadrupole mass spectrometry study of the stabilities and transformations of four alisols in Alismatis Rhizoma and proprietary traditional Chinese medicine prescriptions.Phcog Mag 2018;14:597-603


How to cite this URL:
Xiao CX, He F, Yang MT, Xia F, Tang WJ, Zhou BJ, Zhou H. Ultra-high performance liquid chromatography/triple quadrupole mass spectrometry study of the stabilities and transformations of four alisols in Alismatis Rhizoma and proprietary traditional Chinese medicine prescriptions. Phcog Mag [serial online] 2018 [cited 2021 Apr 19 ];14:597-603
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2018;volume=14;issue=58;spage=597;epage=603;aulast=Xiao;type=0