Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 14  |  Issue : 58  |  Page : 519--524

Molecular cloning and expression analysis of the circadian clock for Patchoulol Synthase gene in Pogostemon Cablin (Blanco) benth


Xindan Liu, Menghua Wu, Ying Zhang, Hui Cao 
 Research Center for Traditional Chinese Medicine of Lingnan, College of Pharmacy, Jinan University, Guangzhou, China

Correspondence Address:
Hui Cao
College of Pharmacy, Jinan University, 601 W. Huangpu Avenue, Guangzhou
China
Ying Zhang
College of Pharmacy, Jinan University, 601 W. Huangpu Avenue, Guangzhou
China

Background: The circadian clock for patchoulol synthase (PTS) gene in Pogostemon cablin remains largely unexplored. Objective: The objective of this study is to clone and to investigate the expression of PTS gene from the leaves of P. cablin throughout a day. Materials and Methods: The full-length PTS cDNA was isolated by a combination of reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends in P. cablin. Gene expression profiles across leaves at different time points of P. cablin were evaluated using quantitative RT-PCR. Results: The PTS clone contained a 1659-bp open reading frame coding for 552 amino acids, a 61-bp 5′-untranslated end, and a 219-bp 3′-untranslated sequence. The deduced amino acid sequence was 47%–63% identical with the sequence of other known sesquiterpene cyclases from angiosperms. It was observed that the oscillations of PTS transcript persisted in P. cablin during the daily cycle, with peak expression occurring at 1:00 h, 5:00 h, 7:00 h, 9:00 h, 16:00 h, 19:00 h and 22:00 h. In general, PTS had lower oscillation frequency and transcription abundance at daytime than at night, corresponding with the higher temperature, the lower humidity, and the presence of an ultraviolet index. Conclusion: The PTS gene identified in this study is under clock control, which in turn may provide insight into evolutionary progresses in the green lineage. Abbreviations used: PTS: Patchoulol synthase; EC: Evening complex; FDP: Farnesyl diphosphate; RACE: Rapid amplification of cDNA ends; CMA: China Meteorological Administration.


How to cite this article:
Liu X, Wu M, Zhang Y, Cao H. Molecular cloning and expression analysis of the circadian clock for Patchoulol Synthase gene in Pogostemon Cablin (Blanco) benth.Phcog Mag 2018;14:519-524


How to cite this URL:
Liu X, Wu M, Zhang Y, Cao H. Molecular cloning and expression analysis of the circadian clock for Patchoulol Synthase gene in Pogostemon Cablin (Blanco) benth. Phcog Mag [serial online] 2018 [cited 2021 Apr 17 ];14:519-524
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2018;volume=14;issue=58;spage=519;epage=524;aulast=Liu;type=0