Year : 2018 | Volume
: 14 | Issue : 58 | Page : 471--476
Lactobacillus plantarum attenuates oxidative stress and liver injury in rats with nonalcoholic steatohepatitis
Duangporn Werawatganon1, Kanjana Somanawat1, Somying Tumwasorn2, Naruemon Klaikeaw3, Prasong Siriviriyakul1
1 Department of Physiology, Alternative and Complementary Medicine for Gastrointestinal and Liver Diseases Research Unit, Bangkok, Thailand
2 Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
3 Department of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
Background: Steatohepatitis is a morphological pattern of liver injury that, in non-alcoholic patients, may represent a form of chronic liver disease currently known as non-alcoholic steatohepatitis (NASH). Probiotics, Lactobacillus sp. and Bifidobacterium sp., have been proposed to prevent and treat different inflammatory conditions of the gastrointestinal tract. Objective: To examine the effect of Lactobacillus plantarum (L. plantarum) on the liver damage of non-alcoholic steatohepatitis (NASH) rats. Materials and Methods: Male Sprague-Dawley rats were randomly divided into three groups. Group 1 (control, n = 8) was fed with phosphate-buffered saline (PBS) 1 mL/rat. Group 2 (NASH, n = 8) was fed with 100% fat diet for 6 weeks. Group 3 (NASH + L. plantarum, n = 8) was fed with 100% fat diet plus L. plantarum 1.8 × 109 colony-forming unit/mL was suspended in PBS by gavage twice a day at an interval of 4 h for 6 weeks. All rats were sacrificed to collect blood and liver samples at the end of the treatment period. Results: The levels of hepatic malondialdehyde (MDA) and tumor necrosis factor alpha (TNF-α) were increased while the expression of peroxisome proliferator activated receptors gamma (PPAR-γ) was decreased significantly in the NASH group as compared with the control group. Histopathology from the NASH group showed macrovesicular steatosis, hepatocyte ballooning, and lobular inflammation. The NASH + L. plantarum group had attenuated the levels of MDA and TNF-α, enhanced PPAR-γ expression, and improved the histopathology. Conclusion: L. plantarum treatment can attenuate oxidative stress, inflammation, and improvement of histopathology in rats with NASH.
Abbreviations used: NAFLD: Nonalcoholic fatty liver disease; NASH: Nonalcoholic steatohepatitis; TNF-α: Tumor necrosis factor-alpha; MDA: Malondialdehyde; PPARγ: Peroxisome proliferator-activated receptor gamma; TBARS: Thiobarbituric acid-reactive substances; ELISA: Enzyme-linked immunosorbent assay.
Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330
|How to cite this article:|
Werawatganon D, Somanawat K, Tumwasorn S, Klaikeaw N, Siriviriyakul P. Lactobacillus plantarum attenuates oxidative stress and liver injury in rats with nonalcoholic steatohepatitis.Phcog Mag 2018;14:471-476
|How to cite this URL:|
Werawatganon D, Somanawat K, Tumwasorn S, Klaikeaw N, Siriviriyakul P. Lactobacillus plantarum attenuates oxidative stress and liver injury in rats with nonalcoholic steatohepatitis. Phcog Mag [serial online] 2018 [cited 2021 Apr 14 ];14:471-476
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2018;volume=14;issue=58;spage=471;epage=476;aulast=Werawatganon;type=0