Year : 2018 | Volume
: 14 | Issue : 57 | Page : 346--351
Phytochemical investigation and bioactivities of Alternanthera ramosissima (Mart.) Chodat and Hassl.
Sukanya Dej-adisai, Sathianpong Phoopha, Jindaporn Puripattanavong
Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand
Background: Since there are many researches of phytochemical and bioactivities from genus Alternanthera but did not have any reports from Alternanthera ramosissima. Objective: The objective of the study is to isolate, identify, and determine the bioactivities of the chemicals in this plant, which has not been reported. Materials and Methods: Bioactivity studies of A. ramosissima ethanol extracts were as cytotoxic activity against human cancer cells (human breast carcinoma cell (MCF-7), human cervical carcinoma cell (KB), human cervix adenocarcinoma cell (HeLa) and human colon adenocarcinoma cell (HT-29)) and anti-inflammation. Chromatographic and spectroscopic techniques were used for the isolation and identification of pure compounds. Results: A. ramosissima extracts had the potential effects of cytotoxic activity against human cancer cells and anti-inflammation. Six compounds were isolated; two mixture compounds as the mixture of β-sitosterol and stigmasterol, the mixture of spinasterol and stigmast-7-en-3 β-ol; and four pure compounds as β-sitosterol-D-glucoside, 7-O-β-D-glucopyranosyl chrysoeriol, 7-O-(6”-O-Acetyl)-β-D-glucopyranosyl chrysoeriol, and kaempferol-3-O-β-rutinoside. The isolated compounds were determined the cytotoxic activity against human cancer cells and anti-inflammatory activity. The results showed that all of them did not have cytotoxic activity against human cancer cells. However, the isolated compounds; 7-O-β-D-glucopyranosyl chrysoeriol and 7-O-(6”-O-Acetyl)-β-D-glucopyranosyl chrysoeriol exhibited the potential effect of anti-inflammation by nitric oxide inhibition with IC50as 25.30 and 39.81 μM, respectively, when compared with the positive standards; indomethacin, caffeic acid phenethyl ester, and L-nitroarginine which showed nitric oxide inhibition with IC50as 50.30, 5.62, and 61.80 μM, respectively. Conclusion: This is the first report of chemicals and bioactivities of A. ramosisima and the first report of cytotoxic and anti-inflammatory activities of isolated compounds; 7-O-β-D-glucopyranosyl chrysoeriol and 7-O-(6”-O-Acetyl-)-β-D-glucopyranosyl chrysoeriol.
Abbreviations used: MCF-7: Human breast carcinoma cell, KB: Human cervical carcinoma cell, HeLa: Human cervix adenocarcinoma cell, HT-29: Human colon adenocarcinoma cell, HGF: Human gingival fibroblast cell, IC50: The half maximal inhibitory concentration, DMEM: Dulbecco's Modified Eagle Medium, cDMEM: Completed DMEM medium, DMSO: Dimethyl sulfoxide, SRB: Sulforhodamine B, NO: Nitric oxide, EIMS: Electron ionized mass spectrometry, NMR: Nuclear magnetic resonance, 1H NMR: Proton NMR, 13C NMR: Carbon-13 NMR.
Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112
|How to cite this article:|
Dej-adisai S, Phoopha S, Puripattanavong J. Phytochemical investigation and bioactivities of Alternanthera ramosissima (Mart.) Chodat and Hassl. Phcog Mag 2018;14:346-351
|How to cite this URL:|
Dej-adisai S, Phoopha S, Puripattanavong J. Phytochemical investigation and bioactivities of Alternanthera ramosissima (Mart.) Chodat and Hassl. Phcog Mag [serial online] 2018 [cited 2021 Mar 8 ];14:346-351
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2018;volume=14;issue=57;spage=346;epage=351;aulast=Dej-adisai;type=0