Year : 2018 | Volume
: 14 | Issue : 56 | Page : 383--389
Protective effect of ethanol extract of Cuscuta chinensis on lipopolysaccharide-induced acute kidney injury via suppressing the toll-like receptors 4-nuclear factor-κB pathway
Wu Song1, Yuan Tao2, Xiaofeng Yang2, Xin-yu Bai3, Shuang Jiang1
1 Department of Pharmacology, College of Basic Medicine, Changchun University of Chinese Medicine, No. 1035 Boshuo Road, Changchun 130117, China
2 Department of Pharmacology, Section of Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China
3 Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, Guizhou 563006, China
Background: The seed of Cuscuta chinensis Lam. (Cuscuta, Convolvulaceae), is widely used in traditional Chinese medicine, with a variety of biological activity. However, the efficacy of C. chinensis has not been investigated in renal injury caused by sepsis. Objective: Our research was carried out to evaluate the effect of ethanol extract of C. chinensis seeds (ECCS) on lipopolysaccharide (LPS)-induced acute kidney injury (AKI) in Kunming mice, and explore its underlying mechanisms. Materials and Methods: ECCS was administered orally (10, 30, and 100 mg/kg bodyweight, once daily) to the AKI mice for 14 consecutive days. On the 14th day, LPS (7 mg/kg) was administered to induce AKI. Blood urea nitrogen (BUN), creatinine (Cr), cytokines, and antioxidant index were estimated. The protein phosphorylation was tested by Western blot. Results: Results indicated that ECCS (100 mg/kg) decreased LPS-induced augmented BUN by 58.54% (P < 0.001) and reduced Cr by 61.57% (P < 0.01), respectively. In addition, the evident reversion of renal pathological damage was observed in ECCS-pretreated mice. ECCS (100 mg/kg) also exhibited a reduction of cytokines (tumor necrosis factor-α, interleukin [IL]-1 β, and IL-6, P < 0.05 for all) in AKI mice. Furthermore, ECCS blocked the activation of the nuclear factor-κB (NF-κB) (p65 and inhibitor kappa B subunit) and reduced the toll-like receptors 4 (TLR4) expression. Conclusions: The protective effect of ECCS against LPS-induced AKI was at least partially associated with suppressing of a TLR4-NF-κB signaling pathway, which provides evidence of the renal protective function of C. chinensis extract.
Abbreviations used: AKI: Acute kidney injury; LPS: Lipopolysaccharide; NF κB: Nuclear factor κB; IκB: Inhibitor kappa B; IL: Interleukin; ECCS: Ethanol extract of C. chinensis seed; SOD: Superoxide dismutase; TLR4: Toll like receptor 4; BUN: Blood urea nitrogen; Cr: Creatinine; MDA: Malondialdehyde; PVDF: Polyvinylidene fluoride; TBST: TBS-Tween solution; iNOS: Inducible NOS; ROS: Reactive oxygen species; TH: T Helper; TBHB: Tert-butyl hydroperoxide; IFN-γ: Interferon-γ.
College of Basic Medicine, Changchun University of Chinese Medicine, No. 1035 Boshuo Road, Changchun 130117
|How to cite this article:|
Song W, Tao Y, Yang X, Bai Xy, Jiang S. Protective effect of ethanol extract of Cuscuta chinensis on lipopolysaccharide-induced acute kidney injury via suppressing the toll-like receptors 4-nuclear factor-κB pathway.Phcog Mag 2018;14:383-389
|How to cite this URL:|
Song W, Tao Y, Yang X, Bai Xy, Jiang S. Protective effect of ethanol extract of Cuscuta chinensis on lipopolysaccharide-induced acute kidney injury via suppressing the toll-like receptors 4-nuclear factor-κB pathway. Phcog Mag [serial online] 2018 [cited 2021 Jun 25 ];14:383-389
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2018;volume=14;issue=56;spage=383;epage=389;aulast=Song;type=0