Year : 2018 | Volume
: 14 | Issue : 56 | Page : 275--282
Yukgunja-tang, a traditional herbal formula, attenuates cigarette smoke-induced lung inflammation in a mouse model
Eunsook Park1, Woo-Young Jeon1, Chang-Seob Seo1, Hyekyung Ha1, Seong Eun Jin1, Jinhee Kim2, Mee-Young Lee1
1 K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon, Korea
2 Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon, Korea
Background: Chronic obstructive pulmonary disease is a progressive lung disease that involves airway inflammation, chronic bronchitis, and emphysema. Yukgunja-tang, one of the traditional Asian herbal medicines, has been used widely in treating patients with gastrointestinal diseases in Korea. Objective: Here, we investigated its efficacy on the inflammatory response using a mouse model of cigarette smoke (CS) exposure together with lipopolysaccharide (LPS) treatment. Materials and Methods: Over 4 weeks, mice were exposed to CS on 5 days/week and instilled intranasally with LPS on days 8 and 23. Yukgunja-tang water extract (YTWE) was administered to mice on the same 5 days. Results: YTWE administration significantly reduced the numbers of inflammatory cells and levels of pro-inflammatory cytokines in bronchoalveolar lavage fluid compared with CS plus LPS-exposed mice. Moreover, YTWE inhibited the phosphorylation of p38 mitogen-activated protein kinase (MAPK) and IκBα proteins induced by CS plus LPS treatment. Histologically, YTWE attenuated the infiltration of inflammatory cells into peribronchial lesions, thickening of alveolar walls and accumulation of collagen in the lung tissues. Conclusion: Our findings suggest that YTWE prevents CS plus LPS-induced lung inflammation by inhibiting p38 MAPK and IκBα signaling. Therefore, YTWE might be a potential drug for the treatment of lung inflammation induced by CS exposure.
Abbreviation used: BALF: Bronchoalveolar lavage fluid; COPD: Chronic obstructive pulmonary disease; CS: Cigarette smoke; HPLC: High-performance liquid chromatography; IL: Interleukin; LPS: Lipopolysaccharide; MAPK: Mitogen-activated protein Kinase; NF-κB: Nuclear factor kappa-B; ROF: Roflumilast; TNF-α: Tumour necrosis factor-alpha; YTWE: Yukgunja-tang water extract.
K-herb Research Center, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon, 34054
|How to cite this article:|
Park E, Jeon WY, Seo CS, Ha H, Jin SE, Kim J, Lee MY. Yukgunja-tang, a traditional herbal formula, attenuates cigarette smoke-induced lung inflammation in a mouse model.Phcog Mag 2018;14:275-282
|How to cite this URL:|
Park E, Jeon WY, Seo CS, Ha H, Jin SE, Kim J, Lee MY. Yukgunja-tang, a traditional herbal formula, attenuates cigarette smoke-induced lung inflammation in a mouse model. Phcog Mag [serial online] 2018 [cited 2021 Apr 19 ];14:275-282
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2018;volume=14;issue=56;spage=275;epage=282;aulast=Park;type=0