Year : 2017 | Volume
: 13 | Issue : 51 | Page : 684--687
Prosopis juliflora pods alkaloid-rich fraction: In vitro anthelmintic activity on goat gastrointestinal parasites and its cytotoxicity on vero cells
Helimar Gonçalves Lima1, Danilo Cavalcante Gomes1, Nathália Silva Santos1, Êuder Reis Dias2, Mariana Borges Botura2, Maria José Moreira Batatinha1, Alexsandro Branco2
1 Department of Pathology and Clinics, School of Veterinary Medicine, Federal University of Bahia, Bahia, Brazil
2 Department of Health, State University of Feira de Santana, Feira de Santana, Bahia, Brazil
Background: This study was designed to assess the in vitro anthelmintic activity of the fraction containing alkaloid from Prosopis juliflora pods on goat gastrointestinal nematodes using the egg hatch assay (EHA), larval migration inhibition assay (LMIA), and larval motility assay (LMA). Materials and Methods: The alkaloid-rich fraction (AF) – content juliprosopine as major alkaloid – was obtained from ethyl acetate extract after fractionation in Sephadex LH-20 chromatography column and its characterization were made by nuclear magnetic resonance analysis together with literature data comparison. The concentrations tested were 4.0, 2.67, 1.78, 1.19, and 0.79 mg/mL (EHA) and 4 mg/mL (LMIA and LMA). The in vitro cytotoxicity on Vero cell cultures was determined with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trypan blue tests. Results: High ovicidal activity was observed with IC50and IC90 values at 1.1 and 1.43 mg/mL for AF. On the other hand, this fraction showed low larvicidal activity and high toxic effect. Conclusion: Thus, P. juliflora pod alkaloid rich-fraction has ovicidal activity in vitro against goat gastrointestinal nematodes and cytotoxic in Vero cell cultures.
Abbreviations used: AF: Alkaloid-rich fraction; DMSO: Dimethyl sulfoxide; EE: Ethyl acetate extract; EHA: Egg hatch assay; IC50: Inhibitory concentration 50%; IC90: Inhibitory concentration 90%; L3: Infective larvae; LMA: Larval motility assay; LMIA: Larval migration inhibition assay; MTT: Bromide 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; NMR: Nuclear magnetic resonance; PBS: Phosphate buffered saline; RPMI: Roswell Park Memorial Institute médium; TLC: Thin Layer Chromatography.
Department of Health, State University of Feira de Santana, Feira de Santana, Bahia
|How to cite this article:|
Lima HG, Gomes DC, Santos NS, Dias &R, Botura MB, Moreira Batatinha MJ, Branco A. Prosopis juliflora pods alkaloid-rich fraction: In vitro anthelmintic activity on goat gastrointestinal parasites and its cytotoxicity on vero cells.Phcog Mag 2017;13:684-687
|How to cite this URL:|
Lima HG, Gomes DC, Santos NS, Dias &R, Botura MB, Moreira Batatinha MJ, Branco A. Prosopis juliflora pods alkaloid-rich fraction: In vitro anthelmintic activity on goat gastrointestinal parasites and its cytotoxicity on vero cells. Phcog Mag [serial online] 2017 [cited 2022 Dec 6 ];13:684-687
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=51;spage=684;epage=687;aulast=Lima;type=0