Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 13  |  Issue : 51  |  Page : 652--657

In vitro protoscolicidal effects of Cinnamomum zeylanicum essential oil and its toxicity in mice


Hossein Mahmoudvand1, Hormoz Mahmoudvand2, Razieh Tavakoli Oliaee3, Amir Tavakoli Kareshk3, Seyed Reza Mirbadie4, Mohammad Reza Aflatoonian5 
1 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
2 Department of Surgery, Lorestan University of Medical Sciences, Khorramabad, Iran
3 Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran
4 Department of Laboratory Sciences, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
5 Bam University of Medical Sciences, Bam; Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran

Correspondence Address:
Mohammad Reza Aflatoonian
Bam University of Medical Sciences, Bam
Iran

Background: This study investigates the scolicidal effects of Cinnamomum zeylanicum essential oil against the protoscoleces of hydatid cysts and its toxicity in the mice model. Materials and Methods: Gas chromatography/mass spectroscopy analyses were used to identify the constituents of essential oil. Protoscoleces were treated with different concentrations of the essential oil (6.25–100 μL/mL) in each test tube for 5–30 min. The viability of protoscoleces was confirmed using eosin exclusion test (0.1% eosin staining). Forty-eight male NMRI mice were also used to determine the toxicity of C. zeylanicum essential oil (0.5–4 mL/kg). Results: The main components were found to be cinnamaldehyde (91.8%), ρ metoxicinamate (1.57%), and α pinene (1.25%). Findings indicate that C. zeylanicum essential oil with the concentrations of 100 and 50 μL/mL killed 100% of protoscoleces after 5 min of exposure. Also, the lower concentrations of C. zeylanicum essential oil motivated a late protoscolicidal effect. The LD50value of intraperitoneal injection of C. zeylanicum essential oil was 2.07 mL/kg body weight after 48 h, and the maximum nonfatal dose was 1.52 mL/kg body weight. The results also showed that there was no significant toxicity following oral administration of C. zeylanicum essential oil for 2 weeks. Conclusion: The results exhibited the favorable scolicidal activity of C. zeylanicum, which could be applied as a natural scolicidal agent in hydatid cyst surgery.


How to cite this article:
Mahmoudvand H, Mahmoudvand H, Oliaee RT, Kareshk AT, Mirbadie SR, Aflatoonian MR. In vitro protoscolicidal effects of Cinnamomum zeylanicum essential oil and its toxicity in mice.Phcog Mag 2017;13:652-657


How to cite this URL:
Mahmoudvand H, Mahmoudvand H, Oliaee RT, Kareshk AT, Mirbadie SR, Aflatoonian MR. In vitro protoscolicidal effects of Cinnamomum zeylanicum essential oil and its toxicity in mice. Phcog Mag [serial online] 2017 [cited 2022 Nov 29 ];13:652-657
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=51;spage=652;epage=657;aulast=Mahmoudvand;type=0