Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 13  |  Issue : 51  |  Page : 363--371

Wound-healing activity of Zanthoxylum bungeanum maxim seed oil on experimentally burned rats


Xiao-Qiang Li1, Rong Kang2, Jun-Cheng Huo3, Yan-Hua Xie3, Si-Wang Wang3, Wei Cao3 
1 Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi'an, Shaanxi 710032, China
2 Department of Natural Medicine, Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an, Shaanxi 710032; Department of Pharmaceutics, School of Pharmacy, Xi'an Medical University, Xi'an, Shaanxi 710032, China
3 Department of Natural Medicine, Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an, Shaanxi 710032, China

Correspondence Address:
Wei Cao
Department of Natural Medicine, Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 169, Changle West Road, Xi'an, Shaanxi 710032
China

Background: The seed oil of Zanthoxylum bungeanum Maxim (ZBSO) is considered to be rich source of fatty acids, mainly oleic and linoleic acids, and has been used for the treatment of burns in Chinese medicine. Objective: We evaluated the healing efficacy of ZBSO and explored its possible mechanism on scalded rats. Materials and Methods: Sprague-Dawley rat models with deep second-degree burns were set up, and ZBSO (500 and 1000 μl/wound) was topically applied twice daily for 7 days and then once daily until wound healing. The therapeutic effects of ZBSO were evaluated by observing wound closure time, decrustation time, wound-healing ratio, and pathological changes. Collagen type-III, matrix metalloproteinase-2 (MMP-2), MMP-9, phospho-nuclear factor-κB (p-NF-κB) p65, inhibitor of NF-κB subunit α p-IκBα, and inhibitor of NF-κB subunit α (IκBα) expression were determined using Western blotting. Results: The ZBSO-treated group showed a higher wound-healing ratio and shorter decrustation and wound closure times than the untreated group. The topical application of ZBSO increased collagen synthesis as evidenced by an increase in hydroxyproline level and upregulated expression of collagen type-III on days 7, 14, and 21 posttreatment. A reduction in MMP-2 and MMP-9 expressions also confirmed the collagen formation efficacy of ZBSO. Furthermore, there was a significant increase in superoxide dismutase levels and a decrease in malondialdehyde levels in ZBSO-treated wounds. ZBSO also decreased tumor necrosis factor alpha, interleukin-1 (IL-1) β, and IL-6 levels in serum, upregulated IκBα, and downregulated p-NF-κB p65 and p-IκBα expression in vivo, indicating the anti-inflammatory action of ZBSO. Conclusion: ZBSO has significant potential to treat burn wounds by accelerating collagen synthesis and the anti-inflammatory cascade of the healing process. Abbreviations used: ECL: Enhanced chemiluminescence; ECM: Extracellular matrix; ELISA: Enzyme-linked immunosorbent assay; GC-MS: Gas chromatography-mass spectrometry; HRP: Horseradish peroxidase; HYP: Hydroxyproline; IκBα: Inhibitor of NF-κB subunit α; IL: Interleukin; MDA: Malondialdehyde; MMP: Matrix metalloproteinase-2; NF-κB: Nuclear factor-κB; SFE: Supercritical fluid extraction; SOD: Superoxide dismutase; SSD: Silver sulfadiazine; TCM: Traditional Chinese medicine; TNF: Tumor necrosis factor.


How to cite this article:
Li XQ, Kang R, Huo JC, Xie YH, Wang SW, Cao W. Wound-healing activity of Zanthoxylum bungeanum maxim seed oil on experimentally burned rats.Phcog Mag 2017;13:363-371


How to cite this URL:
Li XQ, Kang R, Huo JC, Xie YH, Wang SW, Cao W. Wound-healing activity of Zanthoxylum bungeanum maxim seed oil on experimentally burned rats. Phcog Mag [serial online] 2017 [cited 2022 Jan 17 ];13:363-371
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=51;spage=363;epage=371;aulast=Li;type=0