Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 13  |  Issue : 50  |  Page : 258--261

Simultaneous determination of four compounds, campesterol, emodin8-O-β-D-Glucopyranoside, quercetin, and isoquercitrin in Reynoutria sachalinensis by high-performance liquid chromatography-diode array detector


Min Rye Eom1, Jin Bae Weon1, Youn Sik Jung1, Ga Hee Ryu1, Woo Seung Yang1, Choong Je Ma2 
1 Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon 200-701, Republic of Korea
2 Department of Medical Biomaterials Engineering, College of Biomedical Science; Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea

Correspondence Address:
Choong Je Ma
Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Hyoja-2 Dong, Chuncheon 200-701
Republic of Korea

Background: Reynoutria sachalinensis is a well-known and used herbal medicine to treatment of arthralgia, jaundice, amenorrhea, coughs, carbuncles, and sores. Objective: We have developed high-performance liquid chromatography analysis method for simultaneous determination of isolated four compounds, campesterol, emodin8-O-β-D-glucopyranoside, quercetin, and isoquercitrin from R. sachalinensis is. Materials and Methods: The four compounds were separated on Shiseido C18column (S-5 μm, 4.6 mm I.D. ×250 mm) at a column temperature of 25°C. The mobile phase composed of water and methanol with gradient elution system, and flow rate is 1.0 ml/min. The detection wavelength was set at 205 nm. Results: Validation of this analytical method was evaluated by linearity, precision, and accuracy test. This established method had good linearity (R2 > 0.997). The relative standard deviation values of intra- and inter-day testing were indicated that <2%, and accuracy is 91.66%–103.31% at intraday and 91.69%–103.31% at intraday. The results of recovery test were 92.60%–108.99%. Conclusion: In these results, developed method was accurate and reliable to the quality evaluation of campesterol, emodin 8-O-β-D-glucopyranoside, quercetin, and isoquercitrin isolated from R. sachalinensis. Abbreviations used: HPLC: High-performance liquid chromatography, DAD: Diode array detector, LOD: Limit of detection, LOQ: Limit of quantitation, ICH: International Conference on Harmonisation.


How to cite this article:
Eom MR, Weon JB, Jung YS, Ryu GH, Yang WS, Ma CJ. Simultaneous determination of four compounds, campesterol, emodin8-O-β-D-Glucopyranoside, quercetin, and isoquercitrin in Reynoutria sachalinensis by high-performance liquid chromatography-diode array detector.Phcog Mag 2017;13:258-261


How to cite this URL:
Eom MR, Weon JB, Jung YS, Ryu GH, Yang WS, Ma CJ. Simultaneous determination of four compounds, campesterol, emodin8-O-β-D-Glucopyranoside, quercetin, and isoquercitrin in Reynoutria sachalinensis by high-performance liquid chromatography-diode array detector. Phcog Mag [serial online] 2017 [cited 2021 Jun 17 ];13:258-261
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=50;spage=258;epage=261;aulast=Eom;type=0