Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 12  |  Issue : 46  |  Page : 293--296

A new sucrase enzyme inhibitor from Azadirachta indica


Mohamed I. S. Abdelhady1, Usama Shaheen2, Ammar Bader4, Mahmoud A Youns3 
1 Department of Pharmacognosy, Faculty of Pharmacy, Umm Al-Qura University, Makkah 21955, Saudi Arabia; Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Cairo 11795, Egypt
2 Department of Pharmacognosy, Faculty of Pharmacy, Umm Al-Qura University, Makkah 21955, Saudi Arabia; Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt
3 Department of Biochemistry, Faculty of Pharmacy, Helwan University, Cairo 11795, Egypt; Division of Functional Genome Analysis, German Cancer Research Center, Heidelberg, Germany

Correspondence Address:
Mohamed I. S. Abdelhady
Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Cairo 11795, Egypt

Background: Sucrase enzyme inhibitor considered as an oral anti-diabetic therapy that delays the absorption of eaten carbohydrates, reducing the postprandial glucose and insulin peaks to reach normoglycemia. Materials and Methods: Chromatographic fractionation of the hydroalcoholic extract of leaves of Azadirachta indica growing in KSA, followed by in-vitro assay of sucrase enzyme inhibition activity. Results: This investigation led to the isolation of a new remarkable sucrase enzyme inhibitor; 4`-methyl Quercetin-7-O-β-D-glucuronopyranoside (1) alongside with four known compounds; 2,3-hexahydroxydiphenoyl-(α/β)-D-4C1 -glucopyranose (2), Avicularin (3), Castalagin (4) and Quercetin-3-O-glucoside (5). The structure of the new compound (1) was elucidated on the basis of its spectral data, including ESI-MS, UV, 1H NMR, 13C NMR, 1H- 1H COSY, HSQC, NOESY and HMBC. Conclusion: Under the assay conditions, hydroalcoholic extract of A. indica and compounds 1-5 exhibited significant sucrase enzyme inhibitory activity.


How to cite this article:
Abdelhady MI, Shaheen U, Bader A, Youns MA. A new sucrase enzyme inhibitor from Azadirachta indica.Phcog Mag 2016;12:293-296


How to cite this URL:
Abdelhady MI, Shaheen U, Bader A, Youns MA. A new sucrase enzyme inhibitor from Azadirachta indica. Phcog Mag [serial online] 2016 [cited 2022 Aug 15 ];12:293-296
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2016;volume=12;issue=46;spage=293;epage=296;aulast=Abdelhady;type=0