Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 12  |  Issue : 46  |  Page : 195--200

The anticholinesterase properties of plants from the northeast of brazil selected by an ethnopharmacological study for disorders relating to the nervous system


Valerium Thijan Nobre de Almeida e Castro1, Tadeu Jose da Silva Peixoto Sobrinho2, Allan Jonathan Chernichiarro Corrêa1, Thiago Antonio de Sousa Araújo3, Terezinha Gonçalves Da Silva4, Elba Lucia Cavalcanti de Amorim1 
1 Department of Pharmaceutical Sciences, Health Sciences Center, Federal University of Pernambuco, Recife, Brazil
2 University Center Valley Ipojuca, Caruaru, Brazil
3 Health Sciences Center, Federal University of Tocantins, Palmas, Brazil
4 Department of Antibiotics, Health Sciences Center, Federal University of Pernambuco, Recife, Brazil

Correspondence Address:
Valerium Thijan Nobre de Almeida e Castro
Department of Pharmacy, Federal University of Pernambuco, Health Sciences Center, Prof. Arthur de Sa Avenue, Pernambuco, 50670-901
Brazil

Background: Various factors may trigger Alzheimer's disease and the cholinergic hypothesis, which is one of the most widely accepted, argues damage to the brain nuclei, may reduce the production of the choline acetyltransferase enzyme, and cause a decline in the synthesis of acetylcholine (ACh). Studies have thus focused on discovering molecules that are capable of inhibiting the action of cholinesterase enzymes that degrade ACh, thereby preventing the evolution of the disease. Objective: The aim of the present study is to assess the anticholinesterase properties of extracts of medicinal plants in a semi-arid region of Northeast of Brazil. Materials and Methods: The species were selected by way of an ethnobotanical study and were collected if there were some indications that they are related to the nervous system. The plant samples were extracted using hexane, ethyl acetate, and methanol. Anticholinesterase activity in vitro was assessed by way of bioautography in thin layer chromatography and microassays in 96-well plates. Results: Twenty-three species of plant were collected, and 75 extracts were analyzed. The bioautography revealed that 26.7% of the samples showed inhibitory activity against the acetylcholinesterase (AChE) enzyme. After the test for false positives, 8% of the samples were found to inhibit AChE. Thirty samples were analyzed by microassay (500 μg/mL), on which 86.7% showed moderate to powerful anticholinesterase activity. Conclusion: Of the extracts tested, Citrus limonum, Ricinus communis, and Senna occidentalis stand out as was the most promising in terms of anticholinesterase activity and may serve as a guide for the discovery and development of new substances for the treatment of AD. SUMMARY
  • The bioautography revealed that 26.7% of the samples showed inhibitory activity against the acetylcholinesterase enzyme
  • Samples were analyzed by microassay (500 μg/mL), upon which 86.7% showed moderate to powerful anticholinesterase activity
  • Citrus limonum, Ricinus communis, and Senna occidentalis stand out as being the most promising in terms of anticholinesterase activity
  • C. limonum, R. communis, and S. occidentalis may serve as a guide for the discovery and development of new substances for the treatment of Alzheimer's disease.
Abbreviations used: AChE: Acetilcolinesterase Valerium Thijan Nobre de Almeida e Castro



How to cite this article:
de Almeida e Castro VT, Sobrinho TJ, Corrêa AJ, de Sousa Araújo TA, Da Silva TG, de Amorim EL. The anticholinesterase properties of plants from the northeast of brazil selected by an ethnopharmacological study for disorders relating to the nervous system.Phcog Mag 2016;12:195-200


How to cite this URL:
de Almeida e Castro VT, Sobrinho TJ, Corrêa AJ, de Sousa Araújo TA, Da Silva TG, de Amorim EL. The anticholinesterase properties of plants from the northeast of brazil selected by an ethnopharmacological study for disorders relating to the nervous system. Phcog Mag [serial online] 2016 [cited 2021 Jun 18 ];12:195-200
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2016;volume=12;issue=46;spage=195;epage=200;aulast=de;type=0