Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 12  |  Issue : 45  |  Page : 42--46

Essential oil from the resin of Protium heptaphyllum: Chemical composition, cytotoxicity, antimicrobial activity, and antimutagenicity


Ewelyne Miranda de Lima, Didley Sâmia Paiva Cazelli, Fernanda Endringer Pinto, Renata Alves Mazuco, Ieda Carneiro Kalil, Dominik Lenz, Rodrigo Scherer, Tadeu Uggere de Andrade, Denise Coutinho Endringer 
 Department of Pharmaceutical Sciences, University Vila Velha, Av. Comissário José Dantas de Melo, n°21, Boa Vista Vila Velha – Espírito Santo. CEP 29102–920, Brazil

Correspondence Address:
Denise Coutinho Endringer
Department of Pharmaceutical Sciences, University Vila Velha, Av. Comissário José Dantas de Melo, n°21, Boa Vista Vila Velha – Espírito Santo. CEP 29102–920
Brazil

Background: Protium heptaphyllum (Aubl.) March is popularly used as an analgesic and anti-inflammatory agent. Objective: This study aimed to evaluate the chemical composition of P. heptaphyllum essential oil, its cytotoxicity in a breast cancer cell line (MCF-7), antimicrobial activity, and its antimutagenicity in vivo. Materials and Methods: The chemical composition of the essential oil collected in three 3 years was determined by gas chromatography-mass spectrometry. The cytotoxicity was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Annexin V conjugated with fluorescein isothiocyanate, caspase-3, and tumor necrosis factor-alpha (TNF-α) assays were performed to evaluate apoptosis and inflammatory events. The antimutagenic activity at doses of 25, 50, and 100 mg/kg was determined using a micronucleus test in murine bone marrow. Results: The essential oil showed a predominance of monoterpene compounds, being the terpinolene, p-cymene-8-ol, and p-cymene, present in the essential oil extracted in the 3 years. The essential oil showed a protection against cyclophosphamide-induced genotoxicity, and the cytotoxicity index polychromatic erythrocytes/normochromatic erythrocytes ratio in animals treated with oil at all doses (1.34 ± 0.33; 1.15 ± 0.1; 1.11 ± 0.13) did not differ from the negative control animal (1.31 ± 0.33), but from the cyclophosphamide group (0.61 ± 0.12). Cytotoxicity, at a concentration of 40.0 μg/mL, and antimicrobial activity were not observed for the essential oil (minimum inhibitory concentration ≥0.5 mg/mL). The essential oil did not change the levels of caspase-3 in the TNF-α level. Conclusion: The essential oil showed antimutagenic activity due to its chemical composition.


How to cite this article:
de Lima EM, Cazelli DS, Pinto FE, Mazuco RA, Kalil IC, Lenz D, Scherer R, de Andrade TU, Endringer DC. Essential oil from the resin of Protium heptaphyllum: Chemical composition, cytotoxicity, antimicrobial activity, and antimutagenicity.Phcog Mag 2016;12:42-46


How to cite this URL:
de Lima EM, Cazelli DS, Pinto FE, Mazuco RA, Kalil IC, Lenz D, Scherer R, de Andrade TU, Endringer DC. Essential oil from the resin of Protium heptaphyllum: Chemical composition, cytotoxicity, antimicrobial activity, and antimutagenicity. Phcog Mag [serial online] 2016 [cited 2022 Aug 18 ];12:42-46
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2016;volume=12;issue=45;spage=42;epage=46;aulast=de;type=0