Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 11  |  Issue : 44  |  Page : 481--487

Selective cytotoxicity and pro-apoptotic activity of stem bark of Wrightia tinctoria (Roxb.) R. Br. in cancerous cells


Shilpee Chaudhary1, Raviraj Anand Devkar2, Deepak Bhere3, Manganahalli Manjunath Setty2, Karkala Sreedhara Ranganath Pai1 
1 Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India
2 Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India
3 Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India; Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA

Correspondence Address:
Karkala Sreedhara Ranganath Pai
Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal - 576 104, Karnataka
India

Background: Wrightia tinctoria (Roxb.) R. Br. is a widely available shrub in India used traditionally in various ailments, including cancer. However, the anticancer activity of the bioactive fractions has not been validated scientifically. Objective: To investigate the anticancer potential of stem bark of W. tinctoria and establish its phytochemical basis. Materials and Methods: The ethanol extract and subsequent fractions, petroleum ether, ethyl acetate, n-butanol, and aqueous were prepared by standard methods. In vitro cytotoxicity was determined in MCF-7 (breast) and HeLa (cervical) adenocarcinoma cells, and V79 (nontumor fibroblast) cells and apoptogenic activity in MCF-7 cells by acridine orange (AO)/ethidium bromide (EB) staining. Additionally, the antioxidant potential was evaluated using suitable methods. High-performance thin layer chromatography (HPTLC) analysis was performed for identification of active phytoconstituents. Results: Petroleum ether and ethyl acetate fractions were most potent with IC50values of 37.78 and 29.69 μg/ml in HeLa and 31.56 and 32.63 μg/ml in MCF-7 cells respectively in the sulforhodamine B assay. Comparable results were obtained in HeLa cells in 3-(4,5-dimethylthiazolyl-2-yl)-2,5-diphenyl tetrazolium bromide assay and interestingly, the fractions were found to be safe to noncancerous fibroblast cells. Both fractions induced significant (P < 0.05) apoptotic morphological changes observed by AO/EB staining. Moreover, extract/fractions exhibited excellent inhibition of lipid peroxidation with the ethyl acetate fraction being most active (IC50: 23.40 μg/ml). HPTLC confirmed the presence of two anti-cancer triterpenoids, lupeol, and β-sitosterol in active fractions. Conclusion: Extract/fractions of W. tinctoria exhibit selective cytotoxicity against cancerous cells that is mediated by apoptosis. Fractions are less toxic to noncancerous cells; hence, they can be developed as safer chemopreventive agents.


How to cite this article:
Chaudhary S, Devkar RA, Bhere D, Setty MM, Pai KS. Selective cytotoxicity and pro-apoptotic activity of stem bark of Wrightia tinctoria (Roxb.) R. Br. in cancerous cells.Phcog Mag 2015;11:481-487


How to cite this URL:
Chaudhary S, Devkar RA, Bhere D, Setty MM, Pai KS. Selective cytotoxicity and pro-apoptotic activity of stem bark of Wrightia tinctoria (Roxb.) R. Br. in cancerous cells. Phcog Mag [serial online] 2015 [cited 2021 Apr 18 ];11:481-487
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2015;volume=11;issue=44;spage=481;epage=487;aulast=Chaudhary;type=0