Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 11  |  Issue : 44  |  Page : 352--361

Xanthii Fructus inhibits allergic response in the ovalbumin-sensitized mouse allergic rhinitis model


Nam-Gil Gwak, Eun-Young Kim, Bina Lee, Jae-Hyun Kim, Yong-Seok Im, Ka-Yeon Lee, Chang Jun-Kum, Ho-Seok Kim, Hyun-Joo Cho, Hyuk-Sang Jung, Youngjoo Sohn 
 Department of Anatomy, College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, Seoul, Korea

Correspondence Address:
Youngjoo Sohn
College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, Seoul 130-701
Korea

Background: Xanthii Fructus (XF) is widely used in traditional anti-bacterial and anti-inflammatory Asian medicine. Allergic rhinitis is a common inflammatory disease characterized by markedly increased levels of anti-inflammatory factors and the recruitment of inflammatory cells into the nasal mucosa. We investigated the effects of XF in the allergen-induced rhinitis model. Materials and Methods: Following ovalbumin (OVA)/alum intraperitoneal injection on days 0, 7 and 14, the BALB/c mice (albino, laboratory-bred strain of the house mice) were challenged intranasally with OVA for 10 days a week after the last sensitization. The number of sneezes was recorded for 10 days; additionally, the levels of cytokines, histamine, immunoglobulin E (IgE) and OVA-specific serum IgE were estimated. Eosinophil infiltration, thickness of nasal mucosa and expression of caspase-1 were determined by immunohistochemistry. We also evaluated the effect of XF on the phosphorylation of nuclear factor kappa-B (NF-κB) and inhibitor of nuclear factor kappa B-alpha (IκB-α) in human mast cell-1 (HMC-1), by Western blotting. Results: The administration of XF significantly decreased sneezing and the serum levels of histamine, IgE, OVA-specific IgE, and cytokines such as tumor necrosis factor-alpha (TNF-α), interleukine-1 beta (IL-1β), IL-5, IL-6, monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-2 (MIP-2). XF inhibited the changes in thickness of the nasal septum, influx of eosinophils and expression of capase-1. In addition, XF inhibited the phosphorylation of IκB-α and NF-κB in phorbol-myristate-acetate plus calcium ionophore A23187 (A23187) stimulated HMC-1. Conclusion: This study suggests that XF acts a potent anti-allergic drug which alleviates the allergic responses in ovalbumin-sensitized mouse allergic rhinitis model.


How to cite this article:
Gwak NG, Kim EY, Lee B, Kim JH, Im YS, Lee KY, Jun-Kum C, Kim HS, Cho HJ, Jung HS, Sohn Y. Xanthii Fructus inhibits allergic response in the ovalbumin-sensitized mouse allergic rhinitis model.Phcog Mag 2015;11:352-361


How to cite this URL:
Gwak NG, Kim EY, Lee B, Kim JH, Im YS, Lee KY, Jun-Kum C, Kim HS, Cho HJ, Jung HS, Sohn Y. Xanthii Fructus inhibits allergic response in the ovalbumin-sensitized mouse allergic rhinitis model. Phcog Mag [serial online] 2015 [cited 2021 Apr 11 ];11:352-361
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2015;volume=11;issue=44;spage=352;epage=361;aulast=Gwak;type=0