Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 11  |  Issue : 44  |  Page : 329--336

Apoptosis of AGS human gastric adenocarcinoma cells by methanolic extract of Dictamnus


Hyun Soo Park1, Noo Ri Hong1, Tae Seok Ahn1, Hyungwoo Kim2, Myeong Ho Jung1, Byung Joo Kim1 
1 Division of Longevity and Biofunctional Medicine, Healthy Aging Korean Medical Research Center, Yangsan 626-870, Korea
2 Division of Pharmacology, School of Korean Medicine, Pusan National University, Yangsan 626-870, Korea

Correspondence Address:
Byung Joo Kim
Division of Longevity and Biofunctional Medicine, School of Korean Medicine, Pusan National University, Yangsan 626-870
Korea

Background: The root bark of Dictamnus dasycarpusTurcz has traditionally been used in East Asia to treat skin diseases such as eczema, atopic dermatitis, and psoriasis. However, it has also been reported to exhibit an anti-proliferative effect on cancer cells. Objective: To investigate the anti-cancer effects of a methanol extract of Dictamnus dasycarpusroot bark (MEDD) on AGS cells (a human gastric adenocarcinoma cell-line). Materials and Methods: An 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium assay, a caspase activity assay, cell cycle analysis, mitochondrial membrane potential (MMP) measurements, and western blotting were used to investigate the anti-cancer effects of MEDD on AGS cells. Results: Treatment with MEDD significantly and concentration-dependently inhibited AGS cell growth. MEDD treatment in AGS cells led to increased accumulation of apoptotic sub-G1 phase cells in a concentration-dependent manner. Also, MEDD reduced the expressions of pro-caspase-3, -8 and -9, and increased the active form of caspase-3. Furthermore, subsequent Western blotting revealed elevated levels of poly (ADP-ribose) polymerase protein. MEDD treatment reduced levels of MMP and anti-apoptotic Bcl-2 and Bcl-xL proteins. Pretreatment with SB203580 (a specific inhibitor of p38 mitogen-activated protein kinases), SP600125 (a potent inhibitor of C-Jun N-terminal kinases), or PD98059 (a potent inhibitor of extracellular signal-regulated kinases) did not modify the effects of MEDD treatment. However, pretreatment with LY294002 (a specific inhibitor of Akt) significantly enhanced MEDD-induced cell death. Conclusion: These results suggest that MEDD-mediated cell death is associated with the intrinsic apoptotic pathway and that inhibition of Akt signaling contributes to apoptosis induction by MEDD.


How to cite this article:
Park HS, Hong NR, Ahn TS, Kim H, Jung MH, Kim BJ. Apoptosis of AGS human gastric adenocarcinoma cells by methanolic extract of Dictamnus.Phcog Mag 2015;11:329-336


How to cite this URL:
Park HS, Hong NR, Ahn TS, Kim H, Jung MH, Kim BJ. Apoptosis of AGS human gastric adenocarcinoma cells by methanolic extract of Dictamnus. Phcog Mag [serial online] 2015 [cited 2021 Apr 19 ];11:329-336
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2015;volume=11;issue=44;spage=329;epage=336;aulast=Park;type=0