Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 11  |  Issue : 42  |  Page : 66--72

A novel high-performance liquid chromatography-electron spray ionization-mass spectrometry method for simultaneous determination of guggulsterones, piperine and gallic acid in Triphala guggulu


Ganesh Muguli1, PR Rao Vadaparthi2, B Ramesh2, Vishakante Gowda3, Rangesh Paramesh1, Atul N Jadhav1, K Suresh Babu2 
1 Phytochemistry, Research and Development Center, The Himalaya Drug Company, Makali, Bengaluru, India
2 Natural Products Laboratory, Division of Organic Chemistry-I, Indian Institute of Chemical Technology, Hyderabad, India
3 Department of Pharmaceutics, JSS College of Pharmacy, JSS University, Mysore, India

Correspondence Address:
Dr. K Suresh Babu
Division of Natural Products Chemistry, Indian Institute of Chemical Technology, Hyderabad, Andhra Pradesh
India

DQTriphalaguggulu0DQ is an important Ayurvedic formulation comprising of Guggulu, that is, Commiphora wightii (Arn.) Bhandari as a base wherein powdered fruits of triphala, that is, Phyllanthus emblica L., Terminalia bellirica (Gaertn.) Roxb and Terminalia chebula Retz, along with powdered fruit of Piper longum L. are compounded. This polyherbal preparation has been strongly recommended in chronic inflammation, piles, and fistula. However, due to the complexity of compound formulation standardization of commercial products is challenging. In the present communication marker-based standardization of DQTriphalagugguluDQ preparation using gallic acid (for triphala), piperine (for P. longum L.) and guggulsterones (for guggulu) is reported. These compounds of diverse chemistry were successfully separated on a Waters HR-C18 column by isocratic elution with methanol and water (80:20 v/v) as mobile phase at the flow rate of 1.0 mL/min coupled with photodiode array detector. These optimal chromatographic conditions were used for simultaneous quantification of gallic acid, guggulsterones (E and Z) and piperine in commercial samples by high-performance liquid chromatography-electron spray ionization-mass spectrometry and method was validated as per ICH guidelines.


How to cite this article:
Muguli G, Rao Vadaparthi P R, Ramesh B, Gowda V, Paramesh R, Jadhav AN, Babu K S. A novel high-performance liquid chromatography-electron spray ionization-mass spectrometry method for simultaneous determination of guggulsterones, piperine and gallic acid in Triphala guggulu.Phcog Mag 2015;11:66-72


How to cite this URL:
Muguli G, Rao Vadaparthi P R, Ramesh B, Gowda V, Paramesh R, Jadhav AN, Babu K S. A novel high-performance liquid chromatography-electron spray ionization-mass spectrometry method for simultaneous determination of guggulsterones, piperine and gallic acid in Triphala guggulu. Phcog Mag [serial online] 2015 [cited 2021 Apr 23 ];11:66-72
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2015;volume=11;issue=42;spage=66;epage=72;aulast=Muguli;type=0