Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 11  |  Issue : 41  |  Page : 96--101

Spectrophotometric determination of the total flavonoid content in Ocimum basilicum L. (Lamiaceae) leaves


Layzon Silva, Bianca Ramos Pezzini, Luciano Soares 
 Department of Pharmacy, University of Region of Joinville Univille, Rua Paulo Malschitzki, No. 10, Campus Universitário Zona Industrial, Joinville, Santa Catarina, Brazil

Correspondence Address:
Luciano Soares
Department of Pharmacy, University of Region of Joinville Univille, Rua Paulo Malschitzki, No. 10, Campus Universitário Zona Industrial, Joinville, Santa Catarina
Brazil

Background: The chemical characterization is essential to validate the pharmaceutical use of vegetable raw materials. Ultraviolet spectroscopy is an important technique to determine flavonoids, which are important active compounds from Ocimum basilicum. Objective: The objective of this work was to optimize a spectrophotometric method, based on flavonoid-aluminum chloride (AlCl 3 ) complexation to determine the total flavonoid content (TFC) in leaves of O. basilicum (herbal material), using response surface methodology. Materials and Methods: The effects of (1) the herbal material: Solvent ratio (0.02, 0.03, 0.05, 0.07, and 0.08 g/mL), (2) stock solution volume (0.8, 2.3, 4.4, 6.5, and 8.0 mL) and (3) AlCl 3 volume (0.8, 1.0, 1.2, 1.4, and 1.6 mL) on the TFC were evaluated. The analytical performance parameters precision, linearity and robustness of the method were tested. Results: The herbal material: Solvent ratio and stock solution volume showed an important influence on the method response. After choosing the optimized conditions, the method exhibited a precision (RSD%) lower than 6% for repeatability (RSD%) and lower than 8% for intermediate precision (on the order of literature values for biotechnological methods), coefficient of correlation of 0.9984, and no important influence could be observed for variations of the time of complexation with AlCl 3 . However, the time and temperature of extraction were critical for TFC method and must be carefully controlled during the analysis. Conclusion: Thus, this study allowed the optimization of a simple, fast and precise method for the determination of the TFC in leaves of O. basilicum, which can be used to support the quality assessment of this herbal material.


How to cite this article:
Silva L, Pezzini BR, Soares L. Spectrophotometric determination of the total flavonoid content in Ocimum basilicum L. (Lamiaceae) leaves.Phcog Mag 2015;11:96-101


How to cite this URL:
Silva L, Pezzini BR, Soares L. Spectrophotometric determination of the total flavonoid content in Ocimum basilicum L. (Lamiaceae) leaves. Phcog Mag [serial online] 2015 [cited 2022 May 26 ];11:96-101
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2015;volume=11;issue=41;spage=96;epage=101;aulast=Silva;type=0