Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 11  |  Issue : 41  |  Page : 90--95

Evaluating Nothapodytes nimmoniana population from three localities of Western Ghats using camptothecin as phytochemical marker and selection of elites using a new-content range chart method


Gireesh Ankad1, Vinayak Upadhya1, Sandeep R Pai2, Mansingraj S Nimbalkar3, Harsha V Hegde4, Rajesh K Joshi5, Sanjiva D Kholkute6 
1 Ethnomedicine Division, Indian Council of Medical Research, Nehru Nagar, Belgaum, Karnataka State, India
2 Plant Biotechnology and Tissue Culture Division, Indian Council of Medical Research, Nehru Nagar, Belgaum, Karnataka State, India
3 Department of Botany, Shivaji University, Kolhapur, Maharashtra State, India
4 Ethnomedicine Division; Plant Biotechnology and Tissue Culture Division, Indian Council of Medical Research, Nehru Nagar, Belgaum, Karnataka State, India
5 Phytochemistry Division, Regional Medical Research Centre, Indian Council of Medical Research, Nehru Nagar, Belgaum, Karnataka State, India
6 Ethnomedicine Division; Plant Biotechnology and Tissue Culture Division; Phytochemistry Division, Regional Medical Research Centre, Indian Council of Medical Research, Nehru Nagar, Belgaum, Karnataka State, India

Correspondence Address:
Sandeep R Pai
Regional Medical Research Centre, Indian Council of Medical Research, Nehru Nagar, Belgaum - 590 010, Karnataka State
India

Background: Nothapodytes nimmoniana (Grah.) Mabb. is a high valued medicinal plant endemic to Western Ghats of India, distributed in fragmented populations. The plant is valued for potent anticancer drug camptothecin (CPT). Objective: The study compares and expounds variation in CPT content from leaves and stems of N. nimmoniana obtained from three populations of Western Ghats, India. The study also describes a method for categorizing these populations using content range chart (CRC) method for percent yield of CPT. Materials and Methods: A total of 60 samples were investigated including ten each of leaves and stems from three localities. Micro-extraction method was implemented to extract CPT. reversed phase ultra-performance liquid chromatography photo diode array technique was used to quantify CPT. Results: Leaf samples of an individual collected from Joida, yielded lowest CPT content (0.002 ± 0.000 g/100 g), whereas a stem sample from Amgaon, yielded highest CPT content (0.123 ± 0.006 g/100 g). The findings suggest great variation in individuals producing and accumulating CPT. Using this data along with earlier published work, five categories of CPT yielding plants were made viz. I: Very low: <0.020, II: Low: 0.021-0.039, III: Moderate: 0.040-0.059, IV: High: 0.060-0.079 and V: Very high: >0.080. Based on CPT content in leaves, majority of individuals were under very low category (I st ) and on the other hand stem samples were in SQIISQ category. Besides, very few individuals were observed in category SQVSQ. Conclusion: The study expounds use of CRC method for identifying elite population and suggests the need for its conservation.


How to cite this article:
Ankad G, Upadhya V, Pai SR, Nimbalkar MS, Hegde HV, Joshi RK, Kholkute SD. Evaluating Nothapodytes nimmoniana population from three localities of Western Ghats using camptothecin as phytochemical marker and selection of elites using a new-content range chart method.Phcog Mag 2015;11:90-95


How to cite this URL:
Ankad G, Upadhya V, Pai SR, Nimbalkar MS, Hegde HV, Joshi RK, Kholkute SD. Evaluating Nothapodytes nimmoniana population from three localities of Western Ghats using camptothecin as phytochemical marker and selection of elites using a new-content range chart method. Phcog Mag [serial online] 2015 [cited 2021 Oct 25 ];11:90-95
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2015;volume=11;issue=41;spage=90;epage=95;aulast=Ankad;type=0