Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 11  |  Issue : 41  |  Page : 55--60

Hepatoprotective constituents of Firmiana simplex stem bark against ethanol insult to primary rat hepatocytes


Jung Wha Kim1, Heejung Yang2, Namki Cho1, Bitnarae Kim1, Young Choong Kim1, Sang Hyun Sung1 
1 Department of Pharmacy, College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Korea
2 Department of Pharmacy, College of Pharmacy, Kangwon National University, Korea

Correspondence Address:
Sang Hyun Sung
College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 151-742
Korea

Background: Ethanol causes hepatic cellular damage by alterations in biological functions. This study evaluated the hepatoprotective potential of the methanolic extract originating from Firmiana simplex (Sterculiaceae) stem bark against the ethanol-induced hepatotoxicity in rat primary hepatocytes. Materials and Methods: The extract of F. simplex stem bark was successively fractionated into n-hexane, chloroform, ethyl acetate (EtOAc), and n-butanol. Column chromatography with silica gel and sephadex LH-20 was used to isolate the EtOAc fraction. Rat primary hepatocytes were cultured to study the hepatoprotective activity of isolated substances against ethanol-induced toxicity. Intracellular reactive oxygen species (ROS) levels, the antioxidant activities of glutathione reductase (GR) and glutathione peroxidase (GSH-P X ) enzymes, and the GSH content were measured to examine the antioxidative property of the isolated compounds. Results: Two flavonoid glycosides, quercitrin (1) and tamarixetin 3-O-rhamnopyranoside (2), were isolated from the active EtOAc fraction. Compound 1 significantly protected rat primary hepatocytes against ethanol-induced oxidative stress by reducing the intracellular ROS level and preserving antioxidative defense systems such as GR, GSH-P X , and total GSH. Conclusion: This is the first report on the hepatoprotective activities of the extract of F. simplex. The EtOAc fraction of F. simplex stem bark and its major constituent quercitrin (1) could function as hepatoprotective agents to attenuate the development of alcoholic liver disease.


How to cite this article:
Kim JW, Yang H, Cho N, Kim B, Kim YC, Sung SH. Hepatoprotective constituents of Firmiana simplex stem bark against ethanol insult to primary rat hepatocytes.Phcog Mag 2015;11:55-60


How to cite this URL:
Kim JW, Yang H, Cho N, Kim B, Kim YC, Sung SH. Hepatoprotective constituents of Firmiana simplex stem bark against ethanol insult to primary rat hepatocytes. Phcog Mag [serial online] 2015 [cited 2021 Jun 22 ];11:55-60
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2015;volume=11;issue=41;spage=55;epage=60;aulast=Kim;type=0