Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 11  |  Issue : 41  |  Page : 180--190

Estimation of Costunolide and Dehydrocostus Lactone in Saussurea lappa and its Polyherbal Formulations followed by their Stability Studies Using HPLC-DAD


PR Rao Vadaparthi1, K Kumar1, Vanka Uma Maheswara Sarma1, Qazi Altaf Hussain2, K Suresh Babu1 
1 Division of Natural Products Chemistry, CSIR-Indian Institute of Chemical Technology, Hyderabad, India
2 Department of Horticulture, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Wadura, Sopore, J and K, India

Correspondence Address:
K Suresh Babu
Scientist, Division of Natural Products Chemistry,CSIR Indian Institute of Chemical Technology, Hyderabad - 500 007, Andhra Pradesh
India

Background: Saussurea lappa is one of the popular Ayurvedic herb; costunolide and dehydrocostus lactones are well-known sesquiterpene lactones contained in many plants used as popular herbs, such as S. lappa, and have been considered as potential candidates for the treatment of various types of tumor. Objective: The present study was used for the quantification of costunolide and dehydrocostus lactone in S. lappa and its polyherbal formulations, stability studies of markers and characterization of their degradants. Materials and Methods: HPLC analysis was performed on Waters NOVAPAK HR C 18 column (300 mm Χ 3.9 mm i.d., 6 μm) using isocratic elution with acetonitrile and water (60:40% v/v). Results: The calibration curves of both analytes showed good linearity within the established range 5-100 μg/ml. The limits of detection (LOD) and quantification (LOQ) were 1.5 and 4.6 μg/ml for costunolide and 1.3 and 4.0 μg/ml for dehydrocostus lactone, respectively. Good results were achieved with respect to repeatability (%RSD < 2.0) and recovery (99.3-101.8%). Conclusion: The method was found to be precise, accurate, specific, and was successfully used for analyzing costunolide and dehydrocostus lactone in S. lappa and its polyherbal formulations. The developed method was found to be suitable for stability studies of markers and characterization of their degradation products.


How to cite this article:
Rao Vadaparthi P R, Kumar K, Sarma VU, Hussain QA, Babu K S. Estimation of Costunolide and Dehydrocostus Lactone in Saussurea lappa and its Polyherbal Formulations followed by their Stability Studies Using HPLC-DAD.Phcog Mag 2015;11:180-190


How to cite this URL:
Rao Vadaparthi P R, Kumar K, Sarma VU, Hussain QA, Babu K S. Estimation of Costunolide and Dehydrocostus Lactone in Saussurea lappa and its Polyherbal Formulations followed by their Stability Studies Using HPLC-DAD. Phcog Mag [serial online] 2015 [cited 2021 Sep 25 ];11:180-190
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2015;volume=11;issue=41;spage=180;epage=190;aulast=Rao;type=0