Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 11  |  Issue : 41  |  Page : 117--122

Antihyperglycemic effects of Pandanus amaryllifolius Roxb. leaf extract


Anchalee Chiabchalard1, Nattakarn Nooron2 
1 Department of Clinical Chemistry, Faculty of Allied Health Sciences, Center for Excellence in Omics Nano Medical Technology Development Project, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand
2 Graduate Program in Clinical Biochemistry and Molecular Medicine, Faculty of Allied Health Sciences, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand

Correspondence Address:
Anchalee Chiabchalard
Department of Clinical Chemistry, Faculty of Allied Health Sciences, Center for Excellence in Omics Nano Medical Technology Development Project, Chulalongkorn University, Pathumwan, Bangkok 10330
Thailand

Background: Diabetes mellitus is one of the leading chronic diseases worldwide. In patients with poor glycemic control, high blood glucose level may lead to other life-threatening complications. Pandanus amaryllifolius Roxb. (PA) leaves are used in traditional medicine for the treatment of diabetes. Objective: This study evaluated the effect of crude extract from PA leaves on blood glucose level and the hypoglycemic mechanisms. Materials and Methods: Thirty healthy volunteers were asked to drink PA tea (test-group) or hot water (control group) 15 min after glucose loading (75 g) in a standard oral glucose tolerance test. To study hypoglycemic mechanisms, PA leaves were extracted using two different methods. Method 1; dried PA leaves were extracted with distilled water at 90°C for 15 min, and method 2; dried PA leaves were extracted with 95% ethanol. Both PA extracts were tested for α-glucosidase enzyme inhibition, insulin stimulation, and glucose uptake stimulation. Results: The average of blood glucose level in the control group was 5.55 ± 0.98 mmol/l, while in PA treated group was 6.16 ± 0.79 mmol/l which were statistically different (P < 0.001). The results of antihyperglycemic mechanism showed that PA extracts, prepared both methods, could inhibit α-glucosidase enzyme and induce insulin production in rat pancreatic cell (RINm5F) in dose-dependent manner (P < 0.05). Conclusions: The knowledge gained from this research can be used as a basis for a new drug discovery for the treatment of diabetes.


How to cite this article:
Chiabchalard A, Nooron N. Antihyperglycemic effects of Pandanus amaryllifolius Roxb. leaf extract.Phcog Mag 2015;11:117-122


How to cite this URL:
Chiabchalard A, Nooron N. Antihyperglycemic effects of Pandanus amaryllifolius Roxb. leaf extract. Phcog Mag [serial online] 2015 [cited 2021 Jun 19 ];11:117-122
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2015;volume=11;issue=41;spage=117;epage=122;aulast=Chiabchalard;type=0