Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 10  |  Issue : 40  |  Page : 464--469

A rapid UPLC method for simultaneous determination of eleven components in «SQ»Ge-Gen-Qin-Lian«SQ» decoction


Rui An, Lisha You, Yizhu Zhang, Xinhong Wang, Yuemin Ma 
 Department of Chemistry, College of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China

Correspondence Address:
Rui An
Department of Chemistry, College of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203
China

Background: «SQ»Ge-Gen-Qin-Lian«SQ» Decoction derived from «SQ»Shang-Han-Lun«SQ» compiled by Zhang Zhongjing. It is widely used in the treatment of acute gastroenteritis, bacillary dysentery, virus diarrhea. This paper describes a sensitive and specific assay for the determination of the 11-marker compounds using ultra performance liquid chromatography (UPLC). Objective: To develop an UPLC method for simultaneous determination of 11 bioactive compounds in «SQ»Ge-Gen-Qin-Lian«SQ» preparations. Materials and Methods: The chromatography analysis was performed on an Agilent Proshell 120 EC-C 18 column (4.6 × 50 mm, 2.7 μm) at 30°C with a gradient elution of methanol, 0.5% formic acid and 0.5% ammonium acetate at a flow rate 1.0 ml/min and UV detected at 270 nm. Results: All calibration curves showed good linear regression (r ≥ 0.9993) within tested ranges. Limits of detection (LOD) and limits of quantification (LOQ) fell in the range between 0.0691-1.04 μg/ml and 0.23-3.43 μg/ml, respectively. The mean recovery of each herbal medicine ranged from 96.60 to 102.11%. Conclusion: The method was validated for repeatability, precision, stability, accuracy, and selectivity. The validated method was successfully applied to simultaneous analysis of these active components in «SQ»Ge-Gen-Qin-Lian«SQ» decoction.


How to cite this article:
An R, You L, Zhang Y, Wang X, Ma Y. A rapid UPLC method for simultaneous determination of eleven components in 'Ge-Gen-Qin-Lian' decoction.Phcog Mag 2014;10:464-469


How to cite this URL:
An R, You L, Zhang Y, Wang X, Ma Y. A rapid UPLC method for simultaneous determination of eleven components in 'Ge-Gen-Qin-Lian' decoction. Phcog Mag [serial online] 2014 [cited 2021 Nov 30 ];10:464-469
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2014;volume=10;issue=40;spage=464;epage=469;aulast=An;type=0