Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 10  |  Issue : 38  |  Page : 404--418

Evaluation of antidiabetic, antioxidant and antiglycating activities of the Eysenhardtia polystachya


Rosa Martha Perez Gutierrez1, Efren Garcia Baez2 
1 Laboratory of Research on Natural Products, School of Chemical Engineering and Extractive Industries, Av. Instituto Politécnico Nacional S/N, Unidad Profesional Adolfo Lopez Mateos, Zacatenco, Mexico
2 Laboratory of Research Organic Chemistry. UPIBI IPN, Col. San Pedro Zacatenco, Mexico

Correspondence Address:
Rosa Martha Perez Gutierrez
Laboratory of Research on Natural Products, School of Chemical Engineering and Extractive Industries, Av. Instituto Politécnico Nacional S/N, Unidad Profesional Adolfo Lopez Mateos, Zacatenco, cp 07708
Mexico

Background: Many diseases are associated with oxidative stress caused by free radicals. The aim of the present study was to evaluate the antidiabetic, antioxidant and antiglycation properties of Eysenhardtia polystachya (EP) bark methanol-water extract. Materials and Methods : The antioxidant capacities were evaluated by studying in vitro the scavenging of DPPH and ABTS free radical, reactive oxygen species such as RO 2 , O 2· - , H 2 O 2 , OH . , H 2 O 2 , ONOO-, NO, HOCl, 1 O 2 , chelating ability, ORAC, β-carotene-bleaching and lipid peroxidation. The antiglycation activities of EP were evaluated by haemoglobin, bovine serum albumin (BSA)-glucose, BSA-methylglyoxal and BSA-glucose assays. Oral administration of EP at the doses of 100 mg/kg, 200 mg/kg and 400 mg/g was studied in normal, glucose-loaded and antidiabetic effects on streptozotocin-induced mildly diabetic (MD) and severely diabetic (SD) mice. Results: EP showed Hdonor activity, free radical scavenging activity, metal chelating ability and lipid peroxidation Antioxidant activity may be attributed to the presence of phenolic and flavonoid compounds. EP is an inhibitor of fluorescent AGE, methylglyoxal and the glycation of haemoglobin. In STZ-induced diabetic mice, EP reduced the blood glucose, increased serum insulin, body weight, marker enzymes of hepatic function, glycogen, HDL, GK and HK while there was reduction in the levels of triglyceride, cholesterol, TBARS, LDL and G6Pase. Conclusions: Eysenhardtia polystachya possesses considerable antioxidant activity with reactive oxygen species (ROS) scavenging activity and demonstrated an anti-AGEs and hepatoprotective role, inhibits hyperglycemic, hyperlipidemic and oxidative stress indicating that these effects may be mediated by interacting with multiple targets operating in diabetes mellitus.


How to cite this article:
Gutierrez RP, Baez EG. Evaluation of antidiabetic, antioxidant and antiglycating activities of the Eysenhardtia polystachya.Phcog Mag 2014;10:404-418


How to cite this URL:
Gutierrez RP, Baez EG. Evaluation of antidiabetic, antioxidant and antiglycating activities of the Eysenhardtia polystachya. Phcog Mag [serial online] 2014 [cited 2021 Apr 22 ];10:404-418
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2014;volume=10;issue=38;spage=404;epage=418;aulast=Gutierrez;type=0