Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 10  |  Issue : 37  |  Page : 57--64

Optimization of quercitrin and total flavonoids extraction from Herba Polygoni Capitati by response surface methodology


Fengwei Ma1, Yang Zhao1, Xiaojian Gong1, Yu Xie2, Xin Zhou1 
1 The Research Center for Quality Control of Nature Medicine; Key Laboratory for Information System of Mountainous Area and Protection of Ecological Environment of Guizhou province, Guizhou Nomal University, Guiyang, Guizhou, China
2 Guizhou Warmen Pharmaceutical Co., Ltd., Guiyang, Guizhou, China

Correspondence Address:
Xin Zhou
The Research Center for Quality Control of Nature Medicine, Guizhou Normal University, Guiyang, Guizhou, 550 001
China

Objective: To optimize the conditions for extraction of quercitrin and total flavonoids (TF) from Herba Polygoni Capitati (Touhualiao in Chinese) by using response surface methodology (RSM). Materials and Methods: A central composite design (CCD) was adopted to investigate the effects of three independent variables including solvent composition (%), solvent-material ratio (ml/g) and extraction time (min) on the responses, quercitrin and TF yields. Results: The optimized conditions of the extraction are as follows: Ethanol concentration, 65.63%; solvent-material ratio, 10.55:1 (ml/g); extraction time, 54.33 min. The established mathematical model described the factors of experimental parameters well and provided a statistically accurate prediction of the optimum yields of quercitrin and TF. Conclusion: The experimental values agreed with those predicted by the established mathematical model, thus indicating the suitability of the model employed and the success of RSM in optimizing the extraction conditions.


How to cite this article:
Ma F, Zhao Y, Gong X, Xie Y, Zhou X. Optimization of quercitrin and total flavonoids extraction from Herba Polygoni Capitati by response surface methodology.Phcog Mag 2014;10:57-64


How to cite this URL:
Ma F, Zhao Y, Gong X, Xie Y, Zhou X. Optimization of quercitrin and total flavonoids extraction from Herba Polygoni Capitati by response surface methodology. Phcog Mag [serial online] 2014 [cited 2022 Jan 17 ];10:57-64
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2014;volume=10;issue=37;spage=57;epage=64;aulast=Ma;type=0