Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 10  |  Issue : 37  |  Page : 34--39

High-performance liquid chromatography determination and pharmacokinetics of coumarin compounds after oral administration of Samul-Tang to rats


Youn-Hwan Hwang, Won-Kyung Cho, Doorye Jang, Jeong-Ho Ha, Jin Yeul Ma 
 Korean Medicine-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine, South Korea

Correspondence Address:
Jin Yeul Ma
1672 Yuseongdae-ro, Yuseong-gu, Daejeon - 305811
South Korea

Background: Samul-tang has been traditionally used for the treatment of cardiovascular, gynecologic, cutaneous, and chronic inflammation disorders. Although coumarin compounds do have various pharmacological activities and the same may be present in Samul-tang, however there is little information about it. Objective: A simple and sensitive high-performance liquid chromatography (HPLC) method has been developed for the determination of nodakenin, nodakenetin, decursin, decursinol, and decursinol angelate in rat plasma. To obtain a better understanding for pharmacological properties of Samul-tang, pharmacokinetic study of coumarin compounds was performed after oral administration of Samul-tang in rats. Materials and Methods: Chromatographic separation of the analytes was successfully achieved on a Phenomenex Luna C 18 column (4.6 mm×250 mm, 5 μm) using a mobile phase composed of acetonitrile water with a gradient elution at a flow rate of 1 mL/min. Noncompartmental analysis was performed. Results: Calibration curves for all analytes had good linearity (r 2 <0.999) in a wide linear range. The lower limit of quantification (LLOQ) ranged from 0.05 to 0.1 μg/mL. The variation of intra- and interday assay was less than 15%. Nodakenin, nodakenetin, and decursinol were determined in rat plasma after oral administration of Samul-tang. Conclusion: This developed and validated HPLC method was successfully applied to the pharmacokinetic study of three coumarin compounds in rats, given as a single oral administration of Samul-tang. These pharmacokinetic data of the nodakenin, nodakenetin, and decursinol could offer a new point of view to evaluate the pharmacological effects of Samul-tang.


How to cite this article:
Hwang YH, Cho WK, Jang D, Ha JH, Ma JY. High-performance liquid chromatography determination and pharmacokinetics of coumarin compounds after oral administration of Samul-Tang to rats.Phcog Mag 2014;10:34-39


How to cite this URL:
Hwang YH, Cho WK, Jang D, Ha JH, Ma JY. High-performance liquid chromatography determination and pharmacokinetics of coumarin compounds after oral administration of Samul-Tang to rats. Phcog Mag [serial online] 2014 [cited 2022 Jan 17 ];10:34-39
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2014;volume=10;issue=37;spage=34;epage=39;aulast=Hwang;type=0