Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 10  |  Issue : 37  |  Page : 189--197

Multi-component analysis in sun-dried and sulfur-fumigated Angelicae Sinensis Radix by single marker quantitation and chemometric discrimination


Yajing Lou1, Hao Cai2, Xiao Liu2, Gang Cao3, Sicong Tu4, Songlin Li5, Xiaoqing Ma1, Kunming Qin1, Baochang Cai6 
1 Department of Chinese Materia Medica, College of Pharmacy; Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, China
2 Department of Chinese Materia Medica, College of Pharmacy; Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing; National First Class Key Discipline for Science of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing, China
3 Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing; National First Class Key Discipline for Science of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing; Research Center of Traditional Chinese Medicine Processing Technology, Zhejiang Chinese Medical University, Hangzhou, China
4 Faculty of Medicine, University of New South Wales, Sydney, New South Wales, Australia
5 Department of Pharmaceutical Analysis and Metabolomics, Jiangsu Province Academy of Chinese Medicine, Nanjing, China
6 Department of Chinese Materia Medica, College of Pharmacy; Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing; National First Class Key Discipline for Science of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing; Research Center of Traditional Chinese Medicine Processing Technology, Zhejiang Chinese Medical University, Hangzhou, China

Correspondence Address:
Hao Cai
Department of Chinese Materia Medica, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing - 210 023
China

Background: A new method has been developed for the simultaneous determination of ferulic acid, senkyunolide A, and Z-ligustilide in Angelicae Sinensis Radix before and after sulfur-fumigation using quantitative analysis of multi-components by a single marker (QAMS). Materials and Methods: The feasibility and accuracy of QAMS were checked by the external standard method, and various high-performance liquid chromatographic instruments and chromatographic conditions were investigated to verify its applicability. Using ferulic acid as the internal reference substance, and the contents of senkyunolide A and Z-ligustilide were calculated according to relative correction factors by high-performance liquid chromatography. Meanwhile, the influence of sulfur-fumigation on these chemical components in Angelicae Sinensis Radix were evaluated and discriminated by chromatographic fingerprint and chemometrics. Results: There was no significant difference observed between the QAMS method and the external standard method. Furthermore, sulfur-fumigation reduced the contents of ferulic acid, senkyunolide A, and Z-ligustilide in Angelicae Sinensis Radix by some degree, and the sun-drying and sulfur-fumigation processing could be easily discriminated by chromatographic fingerprint and chemometrics. Conclusion: QAMS is a convenient and accurate approach to analyzing multi-component when reference substances are unavailable, simultaneously, chemometrics is an effective way to discriminate sun-dried and sulfur-fumigated Angelicae Sinensis Radix.


How to cite this article:
Lou Y, Cai H, Liu X, Cao G, Tu S, Li S, Ma X, Qin K, Cai B. Multi-component analysis in sun-dried and sulfur-fumigated Angelicae Sinensis Radix by single marker quantitation and chemometric discrimination.Phcog Mag 2014;10:189-197


How to cite this URL:
Lou Y, Cai H, Liu X, Cao G, Tu S, Li S, Ma X, Qin K, Cai B. Multi-component analysis in sun-dried and sulfur-fumigated Angelicae Sinensis Radix by single marker quantitation and chemometric discrimination. Phcog Mag [serial online] 2014 [cited 2022 Jan 25 ];10:189-197
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2014;volume=10;issue=37;spage=189;epage=197;aulast=Lou;type=0