Pharmacognosy Magazine

RESEARCH ARTICLE
Year
: 2009  |  Volume : 5  |  Issue : 17  |  Page : 86--91

Tracheal relaxant effect of column chromatographic elutes of chloroform fraction of Adhatoda schimperiana leaves in guinea-pigs


Zelalem Petros1, Eyasu Makonnen1, Asfaw Debella2 
1 Department of Pharmacology, Faculty of Medicine, Addis Ababa University, P.O. Box 9086, Addis Ababa, Ethiopia
2 Department of Drug Research, Ethiopian Health and Nutrition Research Institute, P.O. Box 1242, Addis Ababa, Ethiopia

Correspondence Address:
Zelalem Petros
Department of Pharmacology, Faculty of Medicine, Addis Ababa University, P.O. Box 9086, Addis Ababa
Ethiopia

Adhatoda schimperiana has been used in Ethiopian traditional medicine as a remedy for bronchial asthma. In the present study, tracheal relaxant effect of column chromatographic elutes (CCEs) of the chloroform fraction of the leaves of the plant was investigated on guinea-pigs. The intermediate polar CCE of the chloroform fraction showed tracheal relaxant effect as observed by a right-ward shift of the dose-response curve. The maximum response to histamine in presence of the intermediate polar CCE was significantly lower than that of the chloroform fraction (p<0.05). These results suggest that bioactivity-guided fractionation could show improved tracheal relaxant activity, and the alkaloid-rich fraction of the crude extract might be responsible for the claimed anti-asthmatic effect of the plant.


How to cite this article:
Petros Z, Makonnen E, Debella A. Tracheal relaxant effect of column chromatographic elutes of chloroform fraction of Adhatoda schimperiana leaves in guinea-pigs.Phcog Mag 2009;5:86-91


How to cite this URL:
Petros Z, Makonnen E, Debella A. Tracheal relaxant effect of column chromatographic elutes of chloroform fraction of Adhatoda schimperiana leaves in guinea-pigs. Phcog Mag [serial online] 2009 [cited 2021 Apr 22 ];5:86-91
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2009;volume=5;issue=17;spage=86;epage=91;aulast=Petros;type=0