Pharmacognosy Magazine

RESEARCH ARTICLE
Year
: 2009  |  Volume : 5  |  Issue : 17  |  Page : 71--74

A Sensitive Reversed Phase HPLC Method for the Determination of Curcumin


Yadav Vivek Ramshankar, Sarasija Suresh 
 Department of Pharmaceutics, Al-Ameen college of Pharmacy, Hosur Road, Bangalore - 560027, India

Correspondence Address:
Sarasija Suresh
Department of Pharmaceutics, Al-Ameen college of Pharmacy, Hosur Road, Bangalore - 560027
India

A simple, precise, rapid and accurate, binary reverse phase high performance liquid chromatographic method has been developed for the determination of curcumin a natural drugs with short run time. Chromatographic separation was achieved by using Merck C15 (250 cm X 4.6 mm) Column with mobile phase acetonitril: tetrahydrofuran: 2% acetic acid 50:30:20 (2%) was used. The flow rate was 0.7 ml/min. The retention time was 4.587 minutes. The limit of detection and limit of quantification of curcumin were between 3.68 to 8.125 ng/ml for 50-μL injection volumes. The percentage recovery of curcumin was found to be 97.2 to 98.4 and Relative standard deviation was 0.345 % and 1.160 %. The developed HPLC method can therefore be applied to both in vitro studies of curcumin formulations as well as drug estimation in biological samples.


How to cite this article:
Ramshankar YV, Suresh S. A Sensitive Reversed Phase HPLC Method for the Determination of Curcumin.Phcog Mag 2009;5:71-74


How to cite this URL:
Ramshankar YV, Suresh S. A Sensitive Reversed Phase HPLC Method for the Determination of Curcumin. Phcog Mag [serial online] 2009 [cited 2021 Apr 19 ];5:71-74
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2009;volume=5;issue=17;spage=71;epage=74;aulast=Ramshankar;type=0