Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
Advanced search 
Year : 2021  |  Volume : 17  |  Issue : 75  |  Page : 511-517

Neuroprotective effect of Cucurbita pepo in lipopolysaccharide-induced toxicity in C57BL/6 mice

1 Department of Neurology, General Practice, Ankang Hospital of Traditional Chinese Medicine, Shaanxi, Ankang, China
2 Department of Geriatric, General Practice, Ankang Hospital of Traditional Chinese Medicine, Shaanxi, Ankang, China

Correspondence Address:
Pengli Hui
Department of Geriatric, General Practice, Ankang Hospital of Traditional Chinese Medicine, Shaanxi, Ankang 725000
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/pm.pm_170_20

Rights and Permissions

Background: Although the powerful scientific studies discover Cucurbita pepo probable in the treatment of various ailments, Cucurbita pepo being employed as food material in various part of the world, there is indeterminate in the neuroprotective potential of Cucurbita pepo in LPS induced neuroinflammation experimental model. Aim and Objectives: This study is intended to examine the protective effect of Cucurbita pepo against LPS induced neuro-inflammation in C57BL/6 mice. Materials and Methods: A single dose intraperitoneal (i.p) injection of lipopolysaccharide liquefied in saline was given to C57BL/6 mice. (n=10 per group). Aqueous extract of Cucurbita pepo (AECP) at a concentration of (100 mg/kg b.w.) was administered orally to C57BL/6 mice 1 hour before LPS induction (7 days) and continuous till 30 days (n=10 per group). Results: Levels of enzymatic antioxidants such as catalase (43.26±4.61 %) and superoxide dismutase (43.16±3.82%) restored decidedly and non-enzymatic antioxidants GPx and GSH restored up to 20.81±4.22 and 58.28±2.44 percentage respectively upon AECP treatment. Oxidative stress markers NO and LPO abridged to 57.64±3.17 and 53.25±2.53 percentage compared to LPS induction group. AECP treatment lessened the protein expression level of proinflammatory cytokines Tumor Necrosis Factor-α (TNF-α), Interleukin 1 beta (IL1-β) and Interleukin 6 (IL-6) significantly (P < 0.05) detected by ELISA. Signs of prolong inflammation caused higher expression of isoforms of nitric oxide synthases genes (eNOS, nNOS and iNOS) signifies NO productivity. In the cortex of LPS challenged mice, AECP significantly (P <0.05) condensed the LPS persuaded expressions of eNOS (1.98±0.41 fold), nNOS (1.74±0.26 fold) and iNOS (1.81±0.52 fold) genes. Also, oral administration of AECP significantly (P < 0.05) reduced the expression of ionized calcium binding adaptor molecule (Iba-1) in LPS induced mice brain cortex (30.37%), were additional supports the anti-inflammatory potential of AECP. Conclusion: In summary AECP displays antioxidant and anti-inflammatory potential against LPS induced neuroinflammation. However, the mechanistic insights of Cucurbita pepo in neuroprotection potential have explained in detail.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded90    
    Comments [Add]    

Recommend this journal