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Year : 2021  |  Volume : 17  |  Issue : 73  |  Page : 6-15  

Biological and chemical evaluation of some African plants belonging to Kalanchoe species: Antitrypanosomal, cytotoxic, antitopoisomerase I activities and chemical profiling using ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometer

1 Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt
2 National Center for Natural Products Research and Department of Pharmaceuticals and Drug Delivery, University of Mississippi, University, MS, USA; Department of Pharmacognosy, Faculty of Pharmacy, University of Alexandria, Alexandria, Egypt
3 Department of Zoology, Faculty of Science, Al-Azhar University, Cairo, Egypt
4 Proteomics and Metabolomics Unit, Department of Basic Research, Children's Cancer Hospital, Cairo; Department of Pharmacology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
5 Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
6 Proteomics and Metabolomics Unit, Department of Basic Research, Children's Cancer Hospital, Cairo; Department of Physiology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
7 Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Cairo; Department of Pharmacognosy, Faculty of Pharmacy, Sinai University, Ismailia, Egypt
8 National Center for Natural Products Research and Department of Pharmaceuticals and Drug Delivery, University of Mississippi, University, MS, USA

Date of Submission04-Jun-2020
Date of Decision06-Jul-2020
Date of Acceptance11-Aug-2020
Date of Web Publication15-Apr-2021

Correspondence Address:
Wael Mohamedy Afifi
Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Cairo 11884
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/pm.pm_232_20

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Background: Human African trypanosomiasis is one of the most serious neglected tropical diseases causing fatal symptoms and death. Natural products are a main source for anti-infective metabolites. Objectives: The objective of the study is to evaluate eight different plants belonging to the Kalanchoe species growing in Egypt for antitrypanosomal, antimalarial, antileishmanial, cytotoxic, and antimicrobial activities. Materials and Methods: The antitrypanosomal activity against Trypanosoma brucei; cytotoxic activities against human colon carcinoma, human hepatocyte carcinoma, and human breast adenocarcinoma cell lines; antileishmanial activity against Leishmania donovani; antimalarial activity against Plasmodium falciparum; and antimicrobial activities of all plant extracts have been examined. As well as the identification of the secondary metabolites for the most active extract was performed via ultra performance liquid chromatography coupled to high resolution quadrupole time of flight mass spectrometer operated in negative and positive ionization modes. Results: Among the examined plant extracts, Kalanchoe longiflora leaves extract showed promising activity against T. brucei with an inhibition concentration of sample at 50% fall in absorbance (IC50) value of 17.6 μg/mL. K. longiflora with other extracts exhibited promising cytotoxic activities. Profiling of the polar secondary metabolites of K. longiflora revealed the presence of 47 metabolites including 31 flavonoids, 9 phenolic acids, 4 anthocyanidins, 2 chalcone glucoside, and 1 coumarin. To determine the mechanism of action of K. longiflora extract as a potent antitrypanosomal and cytotoxic agent, we investigate its ability to inhibit topoisomerase I enzyme. K. longiflora extract showed an excellent activity with an IC50 value of 0.148 μg/mL. Conclusion: These interesting results open the door for further research aiming at the development of a successful treatment for Trypanosoma from K. longiflora.

Keywords: African trypanosomiasis, antitopoisomerase I, cytotoxic, Kalanchoe, ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometer

How to cite this article:
Hegazy MM, Metwaly AM, Mostafa AE, Radwan MM, M. Mehany AB, Ahmed E, Enany S, Magdeldin S, Afifi WM, ElSohly MA. Biological and chemical evaluation of some African plants belonging to Kalanchoe species: Antitrypanosomal, cytotoxic, antitopoisomerase I activities and chemical profiling using ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometer. Phcog Mag 2021;17:6-15

How to cite this URL:
Hegazy MM, Metwaly AM, Mostafa AE, Radwan MM, M. Mehany AB, Ahmed E, Enany S, Magdeldin S, Afifi WM, ElSohly MA. Biological and chemical evaluation of some African plants belonging to Kalanchoe species: Antitrypanosomal, cytotoxic, antitopoisomerase I activities and chemical profiling using ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometer. Phcog Mag [serial online] 2021 [cited 2022 Nov 26];17:6-15. Available from: http://www.phcog.com/text.asp?2021/17/73/6/313491


  • Biological investigation of Kalanchoe species growing in Egypt showed both antitrypanosomal and cytotoxic activities at which the responsible secondary metabolites for these activities were identified using advanced chromatographic analysis method, and quantification of the most valuable chemical classes was done for confirmation and explaining of these activities.

Abbreviations used: HCT-116: Human colon carcinoma; HEPG-2: Human hepatocyte carcinoma; MCF-7: Human breast adenocarcinoma; IC50 value: The inhibition concentration of sample at 50% fall in absorbance; Topo I: Topoisomerase I; Topo II: Topoisomerase II; HDAC: Histone deacetylase; WHO: World Health Organization; ESI: Electrospray ionization; RT: Retention time; UPLC/QTOF-MS/MS: Ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometer; DMSO: Dimethyl sulfoxide; IMDM: Iscove's modified Dulbecco's medium; FBS: Fetal bovine serum; KEGG: Kyoto Encyclopedia of Genes and Genomes; a.m.u.: Atomic mass unit; Rham.: Rhamnose; Glu.: Glucose.

   Introduction Top

Human African trypanosomiasis or sleeping sickness is one of the most serious neglected tropical diseases.[1] The protozoan, Trypanosoma brucei, is the cause of human African trypanosomiasis through the bites of a tsetse fly (Glossina species).[2] According to the World Health Organization, T. brucei is endemic in 37 African countries causing fatal symptoms and death. These symptoms happen due to the ability of the parasite to multiply inside the human body, cross the blood–brain barrier, and attack the central nervous system directly.[3] The number of reported deaths in 2015 because of African trypanosomiasis was 3500.[4] In spite of these scary numbers, human African trypanosomiasis is still one of the neglected tropical diseases.[5]

Natural products reported as a source for anti-infective metabolites, either isolated from plants,[6] marine natural products,[7] or endophytic fungal sources.[8],[9] Kalanchoe species belongs to family Crassulaceae (a family of 34 genera and 1410 species).[10] The genus Kalanchoe was established for the first time by Michel Adanson (1763), comprising 125 species.[11] Kalanchoe species were used extensively in different traditional medicines in many regions, especially Africa, China, India, and Brazil.[12] The antiprotozoal activity of different plants belonging to Kalanchoe species has been well documented.[13],[14],[15]

An important link had been emphasized between the antiprotozoal and the cytotoxic activities through different mechanisms, such as inhibition of histone deacetylase (HDAC) enzyme.[16] Antiprotozoal and cytotoxic activities were also exhibited by several synthetic compounds.[17],[18],[19] In addition, several natural products exhibited anticancer and antitrypanosomal activities, such as camptothecin and rebeccamycin, which were found to have the potential to inhibit the activity of topoisomerase I causing an arrest of the proliferation of cancer cells and Trypanosoma cruzi.[20] These findings prompted us to examine the cytotoxic activities for these plant extracts against human colon carcinoma (HCT-116), human hepatocyte carcinoma (HEPG-2), and human breast adenocarcinoma (MCF-7) cell lines.

Topoisomerases are important nuclear enzymes playing a vital role in DNA replication, transcription, chromosome segregation, and recombination.[21] There are two types of topoisomerases: topoisomerase I (Topo I) and topoisomerase II (Topo II). Topo I is responsible for cleavage, relaxing, and releasing of one strand of the DNA duplex, while Topo II cleaves DNA helix simultaneously to remove DNA supercoiling.[22] Accordingly, topoisomerases are considered as important targets for cancer chemotherapy treatments.[23] Topoisomerase inhibitors block the ligation step of the cell cycle, generating single-stranded and double-stranded breaks that harm the integrity of the genome.[24] In addition, Topo I is considered a suitable target for antiprotozoal chemotherapy. Camptothecin has been examined and exerted great activity against trypanosomes and Leishmania through the inhibition of Topo I, leading to the promotion of protein–DNA adducts formation and inhibition of DNA synthesis.[25]

The profiling of plant's secondary metabolites using different mass spectrometric techniques has been progressively applied for medicinal plants analysis.[26] The ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC/QTOF-MS) technique is a recent approach in the field of chromatography. It has the advantage of being fast, sensitive, and high-resolution separation technique.[27]

The initial impetus for the present study is to find out an effective treatment for a serious African disease using some African plants belonging to the Kalanchoe sp. In addition, exploring the mechanism of action and the chemical profile of the most active plant's extract are among our goals in this study.

   Materials and Methods Top

Plant material and extraction

Kalanchoe delagoensis, Kalanchoe daigremontiana, Kalanchoe grandiflora, Kalanchoe longiflora, Kalanchoe marmorata, Kalanchoe orgyalis, Kalanchoe thyrsiflora, and Kalanchoe tubiflora were collected and identified by Botanical team of Al-Orman Botanical Garden, Giza, Egypt, on January 2017 [Figure 1]. A voucher specimen (K-101 to K-108) has been deposited in the Pharmacognosy Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt. Samples of 10 g fresh leaves were prepared for extraction through cutting by a mixer. The cut plants exhaustively extracted with 70% ethanol and sonicated at 30 kHz for 30 min. Then, the samples were filtered; the marc was re-extracted 3 times as described before. The collected extracts were filtrated and dried under reduced pressure at 40°C.
Figure 1: Figures of Kalanchoe species

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Antitrypanosomal assay

A 2-day-old culture of T. brucei in the exponential phase was diluted to 5000 parasites/mL with Iscove's modified Dulbecco's medium (IMDM) according to the described protocol.[28] The maximum permissible limit of dimethyl sulfoxide (DMSO) in the assay was 0.5%. The assays were set up in clear 96-well microplates. For primary screening (single concentration of 20 μg/mL in duplicate), extract dilutions (1 mg/mL) were prepared from the stock extracts (20 mg/mL) in IMDM. Each well received 4 μL of diluted extract sample and 196 μL of the culture volume (total culture volume 200 μl). The plates were incubated at 37°C in 5% CO2 for 48 h. Alamar blue (10 μl) (AbD Serotec, Catalog Number BUF012B) was added to each well, and the plates were incubated further for overnight. Standard fluorescence was measured on a Fluostar Galaxy fluorometer (BMG LabTechnologies) at 544 nm excitation and, 590 nm emission. Pentamidine and α-difluoro methyl ornithine (DFMO) were tested as standard. The extracts that have shown >90% inhibition of T. brucei growth in primary screening were subjected to secondary screening for dose-dependent–response analysis. Active extracts were screened at concentrations ranging from 10 to 0.4 μg/mL. The inhibition concentration of sample at 50% fall in absorbance (IC50) and IC90 values were computed from dose-dependent–response growth inhibition curve by XLfit version 5.2.2.

Antileishmanial assay

The antileishmanial activity of the crude extracts, fractions, and isolated metabolites was tested in vitro against a culture of Leishmania donovani promastigotes using pentamidine and amphotericin B as positive controls.[29]

Antimalarial assay

Crude extracts and fractions were tested on chloroquine-sensitive (D6, Sierra Leone) and chloroquine-resistant (W2, Indo-china) strains of Plasmodium falciparum based on plasmodial lactate dehydrogenase activity (LDH) activity using previously reported method; artemisinin and chloroquine have been used as positive controls.[30]

Antimicrobial assay

The antimicrobial activity of different extracts was screened for their ability to inhibit a panel of five bacteria and five fungi. Those bacteria and fungi are pathogenic to humans including Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), Escherichia coli, Pseudomonas aeruginosa, Mycobacterium intracellulare, Candida albicans, Candida glabrata, Candida krusei, Aspergillus fumigatus, and Cryptococcus neoformans. The antimicrobial assay was carried out according to previously reported method using ciprofloxacin and amphotericin B as positive controls.[30],[31]

Cytotoxic assay

Antiproliferative activity screening was carried out against three cancer cell lines (HCT-116, HEPG-2, and MCF-7 cell lines). The anticancer activity was measured quantitatively using the neutral red assay protocol as described by Borenfreund and Puerner.[32] Briefly, the cell lines were cultured in DMEM (Lonza group) supplemented with 200 mM of L-glutamine and 10% of fetal bovine serum (FBS). The test compounds were dissolved in a mixture of DMSO and DMEM with ratio 4:100 (v/v), respectively. An initial dose of (1 mg/mL) was tested on different cell lines and sub sequenced by seven more dilutions using two-fold dilution factor. Cells were seeded with a concentration of (6 × 104 cell/mL) for 24 h in the flat bottom 96-well plates and incubated at 37°C with 5% CO2 until semi-confluent cell layer was obtained and then treated with 100 μL of each of serially diluted compounds. After 48 h, the anticancer activity of the compounds was measured quantitatively by an ELISA microplate reader at a wavelength of 540 nm using neutral red assay protocol.

Topoisomerase I assay

Kit components

Item specifications (48T/96T) storage

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TOP1 antibody was precoated onto 96-well plates and the biotin-conjugated anti-TOP1 antibody was used as detection antibodies. The standards, test samples, and biotin-conjugated detection antibody were added to the wells subsequently and washed with wash buffer. HRP streptavidin was added, and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TOP1 amount of sample captured in plate. Read the optical density absorbance at 450 nm in a microplate reader and then the concentration of TOP1 can be calculated.

Liquid chromatography-mass spectrometry/mass spectrometry


LC-MS grade acetonitrile and gradient solvents including isopropanol, methanol, dichloromethane, and ethyl acetate were provided by Thermo-Fisher (Thermo Fisher Scientific, USA). Formic acid 98%, ammonium hydroxide, ammonium formate, and ammonium acetate were purchased from Sigma-Aldrich (Sigma-Aldrich Co., Louis St., MO, USA).

Instruments and acquisition method

Separation of small molecules was carried out on an Axion AC system (Kyoto, Japan) connected with an autosampler system, an In-Line filter disks precolumn (0.5 μm × 3.0 mm, Phenomenex, USA), and an Xbridge C18 (3.5 μm × 2.1 mm × 50 mm) column (Waters Corporation, Milford, MA, USA) maintained at 40°C and a flow rate of 300 μL/min. The mobile phase consisted of solution (A) 5 mM ammonium formate in 1% methanol, adjusted to pH = 3.0 using formic acid and solution (B) acetonitrile 100% for the positive mode, while the negative mode solution (C) consisted of 5 mM ammonium formate in 1% methanol, adjusted to pH = 8.0 using ammonium hydroxide.

MS was performed using Triple TOF™ 5600+ system equipped with a Duo-Spray™ source operating in the electrospray ionization (ESI) mode (AB SCIEX, Concord, Canada). Subsequently, the top 15 intense ions were selected for acquiring MS/MS fragmentation spectra after each scan.[33]

Data processing

MS-DIAL 3.70 software (Yokohama, Kanagawa, Japan)[34] was used for non-targeting small molecule comprehensive analysis of the sample. According to the acquisition mode, ReSpect-positive (2737 records) or ReSpect-negative (1573 records) databases were used as reference databases. The identified compounds were retrieved for the pathway analysis using Kyoto Encyclopedia of Genes and Genomes (KEGG)[35] to investigate the integration of different molecule in the plant metabolic pathways.

   Results and Discussion Top

Antiprotozoal assay

The eight Kalanchoe sp. extracts examined for antitrypanosomal, antileishmanial, and antimalarial activities. K. longiflora leaves extract only exhibited its activity against T. brucei with an IC50 value of 17.6 μg/mL.

Antimicrobial assay

The plant extracts were evaluated for their antibacterial and antifungal activities against C. albicans, C. glabrata, C. krusei, A. fumigatus, Cryptococcus neformans, S. aureus, MRSA, E. coli, and P. aeruginosa. Unfortunately, neither one of the tested plant extracts showed any promising activity.

Cytotoxic assay

The well-proven relation between antiprotozoal and cytotoxic activities prompted us to examine the cytotoxic activities of the plant extracts against HCT-116, HEPG-2, and MCF-7 cell lines. Several plant extracts exhibited good activities, against the tested cell lines [Table 1].
Table 1: Cytotoxic activities of the examined plants using different cell lines

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Topoisomerase I inhibitory activity

The most active antitrypanosomal extract (K. longiflora) was evaluated for its inhibitory activity against Topo I enzyme. Staurosporine was used as a positive control in this procedure. The results were recorded as an IC50 calculated from the concentration–inhibition response curve. Topo I was efficiently inhibited by K. longiflora ethanolic extract which displayed excellent inhibitory activity with an IC50 value of 0.148 μg/mL. The inhibitory activity of staurosporine was very near to K. longiflora with an IC50 value of 0.135 μg/mL [Figure 2]. This result was consistent with that of in vitro antitrypanosomal and cytotoxic activities of K. longiflora ethanolic extract. This result indicates that the expected mechanism of action of K. longiflora ethanolic extract as an antitrypanosomal and cytotoxic agent is due to its ability to inhibit Topo I enzyme.
Figure 2: Topoisomerase I inhibitory activity of K. longiflora ethanolic extract against staurosporine

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Profiling of Kalanchoe longiflora secondary metabolites via ultra-performance liquid chromatography–quadrupole-time-of-flight mass spectrometer

The valuable biological effects of K. longiflora ethanolic extract prompted us to identify its phytochemical profile through non-targeted profiling method using ultra-performance liquid chromatography (UPLC) coupled with a high-resolution quadrupole-time-of-flight mass spectrometer (QTOF-MS) operated in the negative and positive ionization modes [Figure 3] and [Figure 4]. The extract was analyzed in both positive and negative-ion ESI MS modes to avoid any change in competitive ionization and suppression effects due to the changes in ESI polarity can often circumvent or significantly alter, revealing otherwise suppressed metabolite signals.
Figure 3: Base peak chromatogram (BPC) of Kalanchoe longiflora ethanolic extract in negative electrospray ionization mode

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Figure 4: Base peak chromatogram (BPC) of Kalanchoe longiflora ethanolic extract in positive electrospray ionization mode

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In total, 30 peaks from K. longiflora ethanolic extract were identified based on their negative-ionization mass spectral data versus 17 in the positive-ion mode [Table 2] and [Figure 5]. A total of 47 secondary metabolites were detected and identified. Metabolites belonged to several natural product classes including 31 flavonoids, nine phenolic acids, four anthocyanidins, one coumarin, one chalcone glycoside, and one dihydrochalcone glucoside.
Table 2: Peak annotations of metabolites in Kalanchoe longiflora ethanol extract using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry in negative and positive ionization modes

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Figure 5: Fragmentation pattern of flavonol

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Identification of flavonoids

The flavonoid glycosides resort to generate [M−H] ions more than [M+H]+ ions. In their MS/MS spectra, losses of glycosyl moieties in both negative- and positive-ion mode could be observed as well as their major characteristic fragment ions due to retro-Diels-Alder fragmentation pathway [Figure 5]. In case of O-flavone glycosides, the common losses of 132, 146, 162, and 176 a.m.u indicated the losses of pentose (arabinose or xylose), rhamnose, hexose (glucose or galactose), and hexuronic acid, respectively. Furthermore, flavonoids tended to lose 28 a.m.u. (CO), 18 a.m.u (H2O), and 15 a.m.u (CH3), suggesting the existence of phenolic hydroxyl and methyl groups, thus allowing the characterization of the flavonoid subgroups.[46],[58]

Among 47 different peaks, flavonoids represented the most abundant class in K. longiflora, with 31 peaks. Seventeen of them were tentatively assigned to flavonol subclass. The presence of seven characteristic parent ion peaks in positive- and negative-ion mode at 741.2239 [M+H]+, 595.1642 [M+H]+, 593.1527 [M−H], 577.1539 [M−H], 433.1832 [M+H]+, 431.099 [M−H], 299.0595 [M−H], and 285.0402 [M−H], corresponding to kaempferol and its glycosides [Table 2]. However, quercetin and its glycosides assigned to four characteristic parent ion peaks at 463.0895 [M−H], 449.108 [M+H]+, 435.1984 [M+H]+, and 301.0011 [M−H] [Table 2]. In addition, parent ion peaks for myricetin, quercetin, and isorhamnetin have been observed. Furthermore, nine flavones ion peaks such as apigenin at 269.0459 [M−H], vitexin at 431.0999 [M−H], acacetin at 285.1099 [M+H]+, acacetin-7-O-rutinoside at 591.136 [M−H], luteolin and its glycosides at 609.1513 [M−H], 449.1561 [M+H]+, and 287.0545 [M+H]+ have been recorded. Further, other subclasses, three metabolites belonging to flavanone (prunin, eriodyctiol, and naringenin), 1 isoflavone (puerarin), and 1 flavononol (taxifolin), have been detected.

Several scientific reports indicated the antiprotozoal activity of kaempferol,[59] which was one of the most three active flavonoids as antiamoebic and antigiardial agent among 18 natural flavonoids.[59] Furthermore, kaempferol and its glycosides were reported before to have antileishmanial activity.[60],[61] Quercetin was described as a potent antileishmanial flavonoid[61] and proved to induce apoptosis of T. brucei previously.[62] In fact, different flavonoids exhibited antiprotozoal activities, for instance, fisetin, 3-hydroxyflavone, luteolin, and quercetin showed promising activities against T. cruzi with IC50 values of 0.6, 0.7, 0.8 and 1.0 μg/mL, respectively.[63] Sakuranetin, which is a flavonoid isolated from the leaves of Baccharis retusa (Asteraceae), exhibited good activities against different species of Leishmania with IC50 values in the range of 20–52 μg/mL.[64] As well as some flavonoids isolated from the aerial parts of Dodonaea viscosa (Sapindaceae) exhibited antileishmanial activities with IC50 value ranging from 16.6 to 19.06 μg/ml.[65]

The well-documented antiprotozoal activities of flavonoids, besides the detection of a high content of flavonoids (especially, these which reported for antiprotozoal effects) in K. longiflora ethanolic extract, suggested that the promising antitrypanosomal activity of K. longiflora may be linked to its flavonoid content.

Identification of phenolic acids

Phenolics are a group of secondary metabolites processing different types of promising biological activities.[66] Phenolic acids are commonly reported metabolites in most of the profiling studies of medicinal plants. Phenolic acids produced generally precursor ion [M−H] corresponding to deprotonated molecule and fragment ion [M-H-44] corresponding to loss of CO2 from the carboxylic acid group.[65] In this study, nine phenolic acids were identified including 2 esterified (chlorogenic acid and rosmarinic acid), 1 phenolic glycoside (1-O-sinapoyl-β-D-glucose), and 6 free (shikimic acid, gentisic acid, p-coumaric acid, caffeic acid, salicylic acid, and sinapyl aldehyde).

The antitrypanosomal activity of phenolic acids was discussed before, for example, gallic acid (the famous plant phenolic) exhibited good antitrypanosomal activity against T. brucei with an LD50 value of 46.9 μM.[67]

The presence of phenolic acids in K. longiflora extract in high concentrations may contribute its antitrypanosomal activity.

Other metabolites

The chemical profiling of K. longiflora extract revealed the presence of four anthocyanidins including delphinidin (aglycon) and 3 other glycosides (tulipanin, kuromanin, and delphinidin-3-O-sambubioside), 1 dihydroxycoumarin (esculetin), 1 dihydrochalcone glucoside (phlorizin), and 1 chalcone glycoside (marein) as shown in [Figure 6].
Figure 6: Some compounds tentatively identified in K. longiflora ethanol extract using Ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometer

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   Conclusion Top

The ethanolic extract of K. longiflora leaves exhibited promising antitrypanosomal activity against T. brucei with an IC50 value of 17.6 ug/ml. In addition, it showed promising cytotoxic activities against HCT-116, HEPG-2, and MCF-7 cell lines. Chemical profiling of the polar secondary metabolites in K. longiflora via UPLC coupled to high-resolution QTOF-MS operated in negative and positive ionization modes resulted in tentative identification of 47 metabolites including 31 flavonoids, 9 phenolic acids, 4 anthocyanidins, 1 coumarin, 1 chalcone, and 1 dihydrochalcone glucoside. The proposed mechanism of action of K. longiflora extract as antitrypanosomal and cytotoxic agent may be through its ability to inhibit Topo I enzyme (IC50 value of 0.148 μg/ml).

These interesting results open the door for further research aiming at the development of a successful treatment for Trypanosoma from K. longiflora.


We are grateful for Drs. Melissa Jacob, Babu Tekwani, and Shabana Khan, for antimicrobial, antileishmanial, and antimalarial.

Financial support and sponsorship


Conflicts of interest

There are no conflicts of interest.

   References Top

Brun R, Blum J, Chappuis F, Burri C. Human African trypanosomiasis. Lancet 2010;375:148-59.  Back to cited text no. 1
Stich A, Abel PM, Krishna S. Human African trypanosomiasis. BMJ 2002;325:203-6.  Back to cited text no. 2
WHO. Human African Trypanosomiasis, Situation and Trends; December, 2017.p. 26-8. Available from: http:// www.who.int/gho/neglected_diseases/human_african_trypanosomiasis/en/.  Back to cited text no. 3
Feigin V. Global, regional, and national life expectancy, all-cause mortality, and cause-specific mortality for 249 causes of death, 1980-2015: A systematic analysis for the Global Burden of Disease Study 2015. Lancet 2016;388:1459-544.  Back to cited text no. 4
Feasey N, Wansbrough-Jones M, Mabey DC, Solomon AW. Neglected tropical diseases. Br Med Bull 2010;93:179-200.  Back to cited text no. 5
Mostafa AE, El-Hela AA, Mohammad AI, Cutler SJ, Ross SA. New triterpenoidal saponins from Koelreuteria paniculata. Phytochem Lett 2016;17:213-8.  Back to cited text no. 6
Bhadury P, Mohammad BT, Wright PC. The current status of natural products from marine fungi and their potential as anti-infective agents. J Ind Microbiol Biotechnol 2006;33:325-37.  Back to cited text no. 7
Metwaly AM, Wanas AS, Radwan MM, Ross SA, ElSohly MA. New α-pyrone derivatives from the endophytic fungus Embellisia sp. Med Chem Res 2017;26:1796-800.  Back to cited text no. 8
Metwaly A, Kadry H, El-Hela A, Mohammad A, Ma G, Cutler S, et al. Antileukemic, antileishmanial and antifungal activities of secondary metabolites from the endophytic fungus Nigrospora sphaerica. Planta Med 2013;79:52.  Back to cited text no. 9
Eggli U. Illustrated Handbook of Succulent Plants: Dicotyledons. Springer-Verlag Berlin Heidelberg: Springer Science & Business Media; 2002.  Back to cited text no. 10
Akulova-Barlow Z. Kalanchoe. Cactus Succulent J 2009;81:268-77.  Back to cited text no. 11
Costa SS, Muzitano MF, Camargo LM, Coutinho MA. Therapeutic potential of Kalanchoe species: Flavonoids and other secondary metabolites. Nat Prod Commun 2008;3:2151-64.  Back to cited text no. 12
Bawm S, Tiwananthagorn S, Lin KS, Hirota J, Irie T, Htun LL, et al. Evaluation of Myanmar medicinal plant extracts for antitrypanosomal and cytotoxic activities. J Vet Med Sci 2010;72:525-8.  Back to cited text no. 13
Guzzi S. Development, stability study and in vivo test of the gel formulation with extract and enriched fraction of Kalanchoe crenata (Andrews) Haworth; 2011.  Back to cited text no. 14
Muzitano MF, Bergonzi MC, De Melo GO, Lage CL, Bilia AR, Vincieri FF, et al. Influence of cultivation conditions, season of collection and extraction method on the content of antileishmanial flavonoids from Kalanchoe pinnata. J Ethnopharmacol 2011;133:132-7.  Back to cited text no. 15
Meinke PT, Liberator P. Histone deacetylase: A target for antiproliferative and antiprotozoal agents. Curr Med Chem 2001;8:211-35.  Back to cited text no. 16
Sperandeo NR, Brun R. Synthesis and biological evaluation of pyrazolylnaphthoquinones as new potential antiprotozoal and cytotoxic agents. Chembiochem 2003;4:69-72.  Back to cited text no. 17
Aponte JC, Verástegui M, Málaga E, Zimic M, Quiliano M, Vaisberg AJ, et al. Synthesis, cytotoxicity, and anti-Trypanosoma cruzi activity of new chalcones. J Med Chem 2008;51:6230-4.  Back to cited text no. 18
Andrzejewska M, Yépez-Mulia L, Cedillo-Rivera R, Tapia A, Vilpo L, Vilpo J, et al. Synthesis, antiprotozoal and anticancer activity of substituted 2-trifluoromethyl- and 2-pentafluoroethylbenzimidazoles. Eur J Med Chem 2002;37:973-8.  Back to cited text no. 19
Zuma AA, Cavalcanti DP, Maia MC, de Souza W, Motta MC. Effect of topoisomerase inhibitors and DNA-binding drugs on the cell proliferation and ultrastructure of Trypanosoma cruzi. Int J Antimicrob Agents 2011;37:449-56.  Back to cited text no. 20
Wang JC. Cellular roles of DNA topoisomerases: A molecular perspective. Nat Rev Mol Cell Biol 2002;3:430-40.  Back to cited text no. 21
Lodish H, Berk A, Zipursky SL, Matsudaira P, Baltimore D, Darnell J. The Role of Topoisomerases in DNA Replication; Molecular Cell Biology, 4th edition, 2000.  Back to cited text no. 22
Pommier Y, Leo E, Zhang H, Marchand C. DNA topoisomerases and their poisoning by anticancer and antibacterial drugs. Chem Biol 2010;17:421-33.  Back to cited text no. 23
Hogan MA, McKinney DS. Comprehensive Review for NCLEX-PN. London, UK,: Pearson; 2012.  Back to cited text no. 24
Bodley AL, Shapiro TA. Molecular and cytotoxic effects of camptothecin, a topoisomerase I inhibitor, on trypanosomes and Leishmania. Proc Natl Acad Sci U S A 1995;92:3726-30.  Back to cited text no. 25
Wolfender JL, Rudaz S, Choi YH, Kim HK. Plant metabolomics: From holistic data to relevant biomarkers. Curr Med Chem 2013;20:1056-90.  Back to cited text no. 26
Khan H, Ali J. UHPLC/Q-ToF-MS technique: Introduction and applications. Lett Org Chem 2015;12:371-78.  Back to cited text no. 27
Räz B, Iten M, Grether-Bühler Y, Kaminsky R, Brun R. The Alamar Blue® assay to determine drug sensitivity of African trypanosomes (Tb rhodesiense and Tb gambiense) in vitro. Acta Trop 1997;68:139-47.  Back to cited text no. 28
Metwaly AM, Ghoneim MM, Musa A. Two new antileishmanial diketopiperazine alkaloids from the endophytic fungus Trichosporum sp. Derpharmachemica 2015;7:322-27.  Back to cited text no. 29
Bharate SB, Khan SI, Yunus NA, Chauthe SK, Jacob MR, Tekwani BL, et al. Antiprotozoal and antimicrobial activities of O-alkylated and formylated acylphloroglucinols. Bioorg Med Chem 2007;15:87-96.  Back to cited text no. 30
Radwan MM, Rodriguez-Guzman R, Manly SP, Jacob M, Ross SA. Sepicanin A-A new geranyl flavanone from Artocarpus sepicanus with activity against methicillin-resistant Staphylococcus aureus (MRSA). Phytochem Lett 2009;2:141-3.  Back to cited text no. 31
Borenfreund E, Puerner JA. Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol Lett 1985;24:119-24.  Back to cited text no. 32
Fayek NM, Farag MA, Abdel Monem AR, Moussa MY, Abd-Elwahab SM, El-Tanbouly ND. Comparative metabolite profiling of four citrus peel cultivars via ultra-performance liquid chromatography coupled with quadrupole-time-of-flight-mass spectrometry and multivariate data analyses. J Chromatogr Sci 2019;57:349-60.  Back to cited text no. 33
Tsugawa H, Cajka T, Kind T, Ma Y, Higgins B, Ikeda K, et al. MS-DIAL: Data-independent MS/MS deconvolution for comprehensive metabolome analysis. Nat Methods 2015;12:523-6.  Back to cited text no. 34
Kanehisa M, Goto S. KEGG: Kyoto Encyclopedia of Genes and Genomes. Nucleic Acids Res 2000;28:27-30.  Back to cited text no. 35
Maazoun AM, Hlel TB, Hamdi SH, Belhadj F, Jemâa JM, Marzouki MN. Screening for insecticidal potential and acetylcholinesterase activity inhibition of Urginea maritima bulbs extract for the control of Sitophilus oryzae (L.). J Asia Pac Entomol 2017;20:752-60.  Back to cited text no. 36
Bylund D, Norström SH, Essén SA, Lundström US. Analysis of low molecular mass organic acids in natural waters by ion exclusion chromatography tandem mass spectrometry. J Chromatogr A 2007;1176:89-93.  Back to cited text no. 37
Abu-Reidah IM, Ali-Shtayeh MS, Jamous RM, Arráez-Román D, Segura-Carretero A. HPLC-DAD-ESI-MS/MS screening of bioactive components from Rhus coriaria L. (Sumac) fruits. Food Chem 2015;166:179-91.  Back to cited text no. 38
Mena P, Calani L, Dall'Asta C, Galaverna G, García-Viguera C, Bruni R, et al. Rapid and comprehensive evaluation of (poly) phenolic compounds in pomegranate (Punica granatum L.) juice by UHPLC-MSn. Molecules 2012;17:14821-40.  Back to cited text no. 39
Sun J, Liang F, Bin Y, Li P, Duan C. Screening non-colored phenolics in red wines using liquid chromatography/ultraviolet and mass spectrometry/mass spectrometry libraries. Molecules 2007;12:679-93.  Back to cited text no. 40
Masike K, Mhlongo MI, Mudau SP, Nobela O, Ncube EN, Tugizimana F, et al. Highlighting mass spectrometric fragmentation differences and similarities between hydroxycinnamoyl-quinic acids and hydroxycinnamoyl-isocitric acids. Chem Cent J 2017;11:29.  Back to cited text no. 41
Chen G, Li X, Saleri F, Guo M. Analysis of Flavonoids in Rhamnus davurica and its antiproliferative activities. Molecules 2016;21:1275.  Back to cited text no. 42
Yang Y, Sun X, Liu J, Kang L, Chen S, Ma B, et al. Quantitative and qualitative analysis of flavonoids and phenolic acids in snow chrysanthemum (Coreopsis tinctoria Nutt.) by HPLC-DAD and UPLC-ESI-QTOF-MS. Molecules 2016;21:1307.  Back to cited text no. 43
Mirali M, Purves RW, Stonehouse R, Song R, Bett K, Vandenberg A. Genetics and biochemistry of zero-tannin lentils. PLoS One 2016;11:e0164624.  Back to cited text no. 44
Braca A, Sinisgalli C, De Leo M, Muscatello B, Cioni PL, Milella L, et al. Phytochemical profile, antioxidant and antidiabetic activities of Adansonia digitata L. (Baobab) from mali, as a source of health-promoting compounds. Molecules 2018;23:3104.  Back to cited text no. 45
Tsimogiannis D, Samiotaki M, Panayotou G, Oreopoulou V. Characterization of flavonoid subgroups and hydroxy substitution by HPLC-MS/MS. Molecules 2007;12:593-606.  Back to cited text no. 46
Farag MA, El Fishawy AM, El-Toumy SA, Amer KF, Mansour AM, Taha HE. Antihepatotoxic effect and metabolite profiling of Panicum turgidum extract via UPLC-qTOF-MS. Pharmacogn Mag 2016;12:S446-S453.  Back to cited text no. 47
Cahliková L, Ali BH, Havliková L, Ločárek M, Siatka T, Opletal L, et al. Anthocyanins of Hibiscus sabdariffa calyces from Sudan. Nat Prod Commun 2015;10:77-9.  Back to cited text no. 48
Sugimoto T, Bamba T, Izumi Y, Nomura H, Shiina T, Fukusaki E. Use of ultra-performance liquid chromatography/time-of-flight mass spectrometry with nozzle-skimmer fragmentation for comprehensive quantitative analysis of secondary metabolites in Arabidopsis thaliana. J Sep Sci 2011;34:3587-96.  Back to cited text no. 49
Tine Y, Renucci F, Costa J, Wélé A, Paolini J. A method for LC-MS/MS profiling of coumarins in Zanthoxylum zanthoxyloides (Lam.) B. zepernich and timler extracts and essential oils. Molecules 2017;22:174.  Back to cited text no. 50
Cavaliere C, Foglia P, Pastorini E, Samperi R, Laganà A. Identification and mass spectrometric characterization of glycosylated flavonoids in Triticum durum plants by high-performance liquid chromatography with tandem mass spectrometry. Rapid Commun Mass Spectrom 2005;19:3143-58.  Back to cited text no. 51
Tine Y, Yang Y, Renucci F, Costa J, Wélé A, Paolini J. LC-MS/MS analysis of flavonoid compounds from Zanthoxylum zanthoxyloides extracts and their antioxidant activities. Nat Prod Commun 2017;12:1865-8.  Back to cited text no. 52
Xiao Y, Liu L, Bian J, Yan C, Ye L, Zhao M, et al. Identification of multiple constituents in shuganjieyu capsule and rat plasma after oral administration by ultra-performance liquid chromatography coupled with electrospray ionization and ion trap mass spectrometry. Acta Chromatogr 2018;30:95-102.  Back to cited text no. 53
Gu D, Yang Y, Abdulla R, Aisa HA. Characterization and identification of chemical compositions in the extract of Artemisia rupestris L. by liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry. Rapid Commun Mass Spectrom 2012;26:83-100.  Back to cited text no. 54
March RE, Miao XS, Metcalfe CD. A fragmentation study of a flavone triglycoside, kaempferol-3-O-robinoside-7-O-rhamnoside. Rapid Commun Mass Spectrom 2004;18:931-34.  Back to cited text no. 55
Bai Y, Zheng Y, Pang W, Peng W, Wu H, Yao H, et al. Identification and comparison of constituents of Aurantii fructus and Aurantii fructus Immaturus by UFLC-DAD-triple TOF-MS/MS. Molecules 2018;23:803.  Back to cited text no. 56
Prasain JK, Jones K, Kirk M, Wilson L, Smith-Johnson M, Weaver C, et al. Profiling and quantification of isoflavonoids in kudzu dietary supplements by high-performance liquid chromatography and electrospray ionization tandem mass spectrometry. J Agric Food Chem 2003;51:4213-8.  Back to cited text no. 57
Pinheiro PF, Justino GC. Structural analysis of flavonoids and related compounds - A review of spectroscopic applications. In: Phytochemicals - A Global Perspective of Their Role in Nutrition and Health. Rao V (ed), InTechOpen Ltd., London, UK ISBN; 2012. pp. 33-56.  Back to cited text no. 58
Calzada F, Meckes M, Cedillo-Rivera R. Antiamoebic and antigiardial activity of plant flavonoids. Planta Med 1999;65:78-80.  Back to cited text no. 59
Marín C, Boutaleb-Charki S, Díaz JG, Huertas O, Rosales MJ, Pérez-Cordon G, et al. Antileishmaniasis activity of flavonoids from Consolida oliveriana. J Nat Prod 2009;72:1069-74.  Back to cited text no. 60
Muzitano MF, Tinoco LW, Guette C, Kaiser CR, Rossi-Bergmann B, Costa SS. The antileishmanial activity assessment of unusual flavonoids from Kalanchoe pinnata. Phytochemistry 2006;67:2071-7.  Back to cited text no. 61
Mamani-Matsuda M, Rambert J, Malvy D, Lejoly-Boisseau H, Daulouède S, Thiolat D, et al. Quercetin induces apoptosis of Trypanosoma brucei gambiense and decreases the proinflammatory response of human macrophages. Antimicrob Agents Chemother 2004;48:924-9.  Back to cited text no. 62
Tasdemir D, Kaiser M, Brun R, Yardley V, Schmidt TJ, Tosun F, et al. Antitrypanosomal and antileishmanial activities of flavonoids and their analogues: In vitro, in vivo, structure-activity relationship, and quantitative structure-activity relationship studies. Antimicrob Agents Chemother 2006;50:1352-64.  Back to cited text no. 63
Nabavi SF, Sureda A, Daglia M, Izadi M, Rastrelli L, Nabavi SM. Flavonoids and Chagas' disease: The story so far! Curr Top Med Chem 2017;17:460-6.  Back to cited text no. 64
Mostafa AE, Atef A, Mohammad AE, Jacob M, Cutler SJ, Ross SA. New secondary metabolites from Dodonaea viscosa. Phytochem Lett 2014;8:10-5.  Back to cited text no. 65
Lindroth RL, Batzli GO. Plant phenolics as chemical defenses: Effects of natural phenolics on survival and growth of prairie voles (Microtus ochrogaster). J Chem Ecol 1984;10:229-44.  Back to cited text no. 66
Koide T, Nose M, Inoue M, Ogihara Y, Yabu Y, Ohta N. Trypanocidal effects of gallic acid and related compounds. Planta Med 1998;64:27-30.  Back to cited text no. 67


  [Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6]

  [Table 1], [Table 2]

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