Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
ORIGINAL ARTICLE
Year : 2021  |  Volume : 17  |  Issue : 6  |  Page : 287-291

Anti-cancer effects of Nepeta Deflersiana Extract (NDE) in estrogen positive and negative forms of breast cancer


Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia

Correspondence Address:
Majed Saad Al Fayi
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha
Saudi Arabia
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_464_20

Rights and Permissions

Aim: This study focused on efficacy of the ethanol extract gained from the aerial parts of Nepeta deflersiana (ND) on breast cancer (BC) cells. Materials and Methods: Shade-dried aerial parts of ND were extracted with ethanol using the Soxhlet extraction method (NDE). MTT assay was performed to assess the cytotoxicity of NDE in normal breast cells (MCF-10A), as well as estrogen receptor (ER) positive (MCF-7) and ER-negative (MDA-MB231) BC cells. Nuclear staining was performed using Hoechst/propidium iodide. Annexin V assays for apoptosis detection and cell cycle analysis were performed by flow cytometry. The effect of NDE on metastasis was analyzed by the transmigration assay. Western blot was used to detect the key signaling proteins. Results: NDE inhibited BC cell proliferation with GI50 values of 154.90 and 202.7 μg/ml for MCF-7 and MDA-MB231 cells, respectively. NDE was non-toxic to normal MCF 10A cells at concentrations up to 1000 μg/ml. NDE induced nuclear condensation/fragmentation in both types of BC cells near the GI50 doses values. An apparent increase in early and late apoptotic populations was confirmed by the flow cytometry. The extract also increased the sub-G0/G1 population in both types of BC cells. Dose-dependent increases in Caspase 3 and Bax levels were also recognized, as were NCE decreases in anti-apoptotic Bcl-2 levels in the same cell lines. Conclusion: NDE possessed excellent anticancer properties against ER-positive and negative BC cells, encouraging further research on the use of this natural product against BC.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed130    
    Printed0    
    Emailed0    
    PDF Downloaded24    
    Comments [Add]    

Recommend this journal