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Year : 2020  |  Volume : 16  |  Issue : 72  |  Page : 745-749

Simultaneous determination of two diterpenoids, continentalic acid and kaurenoic acid, in the water extract of Aralia continentalis and their wound-healing activity

1 Korea Food Research Institute, Jeollabuk-do, Korea
2 College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon, Korea
3 College of Pharmacy, Gachon University, Incheon, Korea
4 College of Pharmacy, Sookmyung Women's University, Seoul, Korea
5 College of Pharmacy, Kangwon National University, Chuncheon, Korea

Correspondence Address:
Seung Hyun Kim
#421 Veritas Hall D, Yonsei Univ. 85 Songdogwahak-ro, Yeonsu-gu, Incheeon 406-840
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/pm.pm_344_20

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Background: The roots of Aralia continentalis have been used in traditional Korean medicine to treat pain, lumbago, lameness, and rheumatism. Objective: Simultaneous determination of two diterpenoids; continentalic acid (CA) and kaurenoic acid, in the water extract of A. continentalis using the high-performance liquid chromatography (HPLC) was established for quality control, as well as evaluation of their wound-healing activity. Materials and Methods: The separation was conducted on YMC hydrosphere C18 column with isocratic elution of 65% acetonitrile. Furthermore, the isolated diterpenoids were screened for their wound-healing activity using keratinocytes (HaCaT cells). Results: The calibration curves were linear over the established range with R2 > 0.9999. The intraday and interday RSDs for each compound were 0.13%–0.89% and 0.14%–0.73%, respectively. The limits of detection and limits of quantification for the two tested diterpenoids were 0.0912, 0.0018 μg/μL, and 0.2764, 0.0056 μg/μL, respectively. In addition, the CA-treated groups showed accelerated wound closure compared to the control group. Conclusion: The HPLC method could be accomplished to the quality control and stable experiment for the preparations consisted of two diterpenoids. Compared to the control group, the CA-treated group showed that wound closure was accelerated, indicating its potential in promoting migration of skin cells which is the most important step of wound closure.

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