ORIGINAL ARTICLE |
|
Year : 2020 | Volume
: 16
| Issue : 72 | Page : 745-749 |
|
Simultaneous determination of two diterpenoids, continentalic acid and kaurenoic acid, in the water extract of Aralia continentalis and their wound-healing activity
Guijae Yoo1, Taek Hwan Lee2, Jae Hyuk Lee3, Kyo Bin Kang4, Heejung Yang5, Yong Kon Park1, Sun Yeou Kim3, Seung Hyun Kim2
1 Korea Food Research Institute, Jeollabuk-do, Korea 2 College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon, Korea 3 College of Pharmacy, Gachon University, Incheon, Korea 4 College of Pharmacy, Sookmyung Women's University, Seoul, Korea 5 College of Pharmacy, Kangwon National University, Chuncheon, Korea
Correspondence Address:
Seung Hyun Kim #421 Veritas Hall D, Yonsei Univ. 85 Songdogwahak-ro, Yeonsu-gu, Incheeon 406-840 Korea
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/pm.pm_344_20
|
|
Background: The roots of Aralia continentalis have been used in traditional Korean medicine to treat pain, lumbago, lameness, and rheumatism. Objective: Simultaneous determination of two diterpenoids; continentalic acid (CA) and kaurenoic acid, in the water extract of A. continentalis using the high-performance liquid chromatography (HPLC) was established for quality control, as well as evaluation of their wound-healing activity. Materials and Methods: The separation was conducted on YMC hydrosphere C18 column with isocratic elution of 65% acetonitrile. Furthermore, the isolated diterpenoids were screened for their wound-healing activity using keratinocytes (HaCaT cells). Results: The calibration curves were linear over the established range with R2 > 0.9999. The intraday and interday RSDs for each compound were 0.13%–0.89% and 0.14%–0.73%, respectively. The limits of detection and limits of quantification for the two tested diterpenoids were 0.0912, 0.0018 μg/μL, and 0.2764, 0.0056 μg/μL, respectively. In addition, the CA-treated groups showed accelerated wound closure compared to the control group. Conclusion: The HPLC method could be accomplished to the quality control and stable experiment for the preparations consisted of two diterpenoids. Compared to the control group, the CA-treated group showed that wound closure was accelerated, indicating its potential in promoting migration of skin cells which is the most important step of wound closure.
|
|
|
|
[FULL TEXT] [PDF]* |
|
 |
|