Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
ORIGINAL ARTICLE
Year : 2020  |  Volume : 16  |  Issue : 71  |  Page : 550-556

Anticancer effects of Calotropis procera latex extract in mcf-7 breast cancer cells


1 Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia
2 Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia
3 Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia

Correspondence Address:
Mohammad Abul Farah
Department of Zoology, College of Science, King Saud University, P. O. Box: 2455, Riyadh 11451
Saudi Arabia
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_156_20

Rights and Permissions

Background: Calotropis procera is a wild growing medicinal plant with many pharmacological properties, arising mainly from its latex, which contains many biologically active compounds, including cardiac glycosides. Objectives: The present study was conducted to isolate a cardiac glycosidal (CG) extract from the latex of C. procera and to assess its potential in inducing anticancer effects on breast cancer cells (MCF-7). Materials and Methods: Cytotoxicity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and morphological changes observations. The generation of intracellular reactive oxygen species (ROS) was evaluated both qualitatively and quantitatively. Flow cytometry technique was used to evaluate apoptosis and autophagy was determined by fluorescence microscopy and Western blotting. Results: The extract significantly (P < 0.05) inhibited the proliferation of MCF-7 cells and that this effect increased in line with concentration. Systemic changes in the morphology of treated cells when compared with control cells were observed. ROS levels were increased by about 1.5 and 1.95-fold at the highest concentration of 75 μg/ml after 12 and 24 h of treatment, respectively. A significant (P < 0.05) increase in the percentage of early and late apoptotic cells were recorded. Autophagy induction in treated MCF-7 was confirmed with the presence of acidic vesicular organelles. Finally, the change in the intracellular localization of light chain 3 (LC3) protein was determined by Western blotting using primary antibodies. A maximum of a 1.76-fold increase was observed in the expression level of the LC3 marker protein. Conclusion: These findings suggest that CG extract increased the levels of intracellular ROS resulting in the induction of cytotoxicity, apoptosis, and autophagy in MCF-7 cells.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed170    
    Printed0    
    Emailed0    
    PDF Downloaded23    
    Comments [Add]    

Recommend this journal