| ORIGINAL ARTICLE |
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| Year : 2020 | Volume
: 16
| Issue : 67 | Page : 13-20 |
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Antioxidant effect of Terminalia arjuna extract against acetaminophen-induced hepatotoxicity via the regulation of cytochrome P450 2E1, phosphatidylinositol-3-kinase/protein kinase B
Senthilganesh P Kannappan1, Gunapriya Raghunath2, Senthilkumar Sivanesan3, Rajagopalan Vijayaraghavan3, Madhankumar Swaminathan3
1 Department of Research and Development, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu; Department of Anatomy, Apollo Institute of Medical Science and Research, Chittoor, Andhra Pradesh, India
2 Department of Anatomy, Saveetha Medical College and Hospital, Chennai, Tamil Nadu, India
3 Department of Research and Development, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India
Correspondence Address:
Senthilganesh P Kannappan
Department of Research and Development, Saveetha Institute of Medical and Technical Sciences, Chennai - 602 105, Tamil Nadu
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/pm.pm_339_19
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Aim: The present study explored the therapeutic in detail antioxidants and effect of aqueous Terminalia arjuna (TA) bark extract against acetaminophen (APAP) induced hepatotoxicity through studies on serum marker enzymes, phosphatidylinositol3kinase/protein kinase B (PI3K/AKT) pathway, CYP2E1 evaluations. Biochemical, antioxidant, cytochrome P450 2E1 (CYP2E1) enzyme, and PI3K/AKT cell signal enzymes were observed with the appropriate methods of study. Materials and Methods: The animals were divided into five groups (each having six animals): control group, Acetaminophen (APAP) toxic group, N-acetylcysteine (NAC) group, TA 250 mg/kg group, and TA 500 mg/kg group. APAP toxic dose of 750 mg/kg body weight was administered along with 0.5% of hydroxypropyl cellulose (vehicle) 24 h before sacrificing the animal. Results: The biochemical, antioxidant, Histopathological, CYP2E1 enzyme, PI3K, AKT protein expression analysis were shown increased antioxidant level, increased PI3K/AKT level, decreased liver function marker level and decreased CYP2E1 level in TA500 mg group compared with APAP toxic group (P < 0.01). The findings suggest that TA (500mg/kg) drug reduced Acetaminophen toxicity via antioxidant and molecular mechanisms. Conclusion: The present study concluded that TA 500 mg/kg high dose is more effective to restore the liver tissue through APAPinduced hepatotoxicity in Wistar albino rats.
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