Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
Advanced search 
Year : 2020  |  Volume : 16  |  Issue : 67  |  Page : 119-127

Astragaloside accelerates fracture healing via modulating miR-122/p53 and miR-221/RUNX2 signaling pathways

Department of Pharmacy, Wendeng Hospital of Traditional Chinese Orthopedics and Traumatology of Shandong Province, Wendeng, Shandong, China

Correspondence Address:
Zhenhai Dong
1 Fengshan Road, Wendeng 264400, Shandong
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/pm.pm_656_18

Rights and Permissions

Background: Astragaloside (AS) has been clinically used in the management of fracture, but the underlying mechanism of AS involved in fracture healing is still unknown, so the objective of our study was to explore the above potential mechanism of AS. Materials and Methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, flow cytometry analysis, real-time polymerase chain reaction, Western blot analysis, computational analysis, luciferase assay, and immunohistochemistry (IHC) were utilized to detect the underlying mechanism of AS involved in fracture healing. Results: Cell growth rate, collagen type I, and cell content of osteoblast and MG-63 cell increased while cell apoptosis decreased in a dose-dependent manner compared with controls. MiR-122 and RUNX2 levels showed a stepwise increase while miR-221 and P53 levels showed a stepwise decline as AS concentration increased in osteoblast and MG-63 cells compared with controls. P53 was a virtual target gene of miR-122. Meanwhile, miR-221 directly regulated RUNX2. AS+ group displayed higher messenger RNA (mRNA) levels of miR-122 and RUNX2 than AS− group, while miR-221 and P53 mRNA levels in AS+ group were much lower than AS− group. Results of IHC showed that P53 protein was lowly expressed, while RUNX2 protein was highly expressed in AS+ group compared with AS− group. Conclusion: We identified the effects of the regulatory relationship between AS, miR-122, miR-221, P53, and RUNX2 and their effects on the apoptosis of osteoblasts.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded166    
    Comments [Add]    

Recommend this journal