Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
ORIGINAL ARTICLE
Year : 2020  |  Volume : 16  |  Issue : 5  |  Page : 517-523

Extraction and volatile compounds profiling of the bioactive fraction of Monochoria hastata (L.) solms


1 Department of Botany, Plant and Microbial Physiology and Biochemistry Laboratory, University of Gour Banga, Malda, West Bengal, India
2 Department of Chemistry, University of Gour Banga, Malda, West Bengal, India

Correspondence Address:
Vivekananda Mandal
Department of Botany, Plant and Microbial Physiology and Biochemistry Laboratory, University of Gour Banga, Malda - 732 103, West Bengal
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_386_19

Rights and Permissions

Background: An aquatic macrophyte Monochoria hastata (L.) Solms has ethnomedicinal application in various ailments and experimentally is proved to have antibacterial property. Objectives: To evaluate the optimized extraction methods and solvents for antibacterial activity and their characterization by gas chromatography-mass spectrometry (GC-MS). Materials and Methods: Extractions from the aerial parts of the plant in solvents with different polarities and various techniques, viz., microwave, infusion, Soxhlet, and maceration, were used to evaluate the best antibacterial efficacy by agar well diffusion method. The phytochemical constituents present in the bioactive extract were analyzed using standard phytochemical screening methods and characterized by GC-MS analysis. Results: Ethyl acetate extract derived by the Soxhlet method showed the highest antibacterial activity against all the test bacterial strains, and Gram-positive strains were more susceptible than Gram-negative strains. The crude extracts showed antibacterial activity which ranged from 7.0 ± 0.3 to 16.5 ± 0.8 mm at 100 mg/ml. Fifty percent of methanol had the highest extractive value (21.3%) in the Soxhlet method. Phytochemical tests showed the presence of alkaloids, phenols, flavonoids, terpenoids, glycosides, and fats in the bioactive ethyl acetate extract. GC-MS analysis showed the presence of different fatty acids and their saturated esters as the principal components. The major compounds were tridecanoic acid, methyl ester, 2-hexyldecanoic acid, dodecanoic acid, and diethyl phthalate. Conclusion: The results suggest that the ethyl acetate extract of M. hastata (L.) Solms possesses some bioactive volatile compounds including fatty acids and their esters, which have antibacterial potentiality. This is the first report of such antibacterial assessment from M. hastata (L.) Solms.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed248    
    Printed2    
    Emailed0    
    PDF Downloaded58    
    Comments [Add]    

Recommend this journal