| ORIGINAL ARTICLE |
|
| Year : 2019 | Volume
: 15
| Issue : 65 | Page : 693-697 |
|
|
Estrogenic activity of glycosides from Cistanche deserticola as an estrogen receptors adjuvant in vitro
Hui Song1, Wen-Lan Li2, Xiang-Ming Sun2, Yang Hu2, Jing-Xin Ding3, Yu-Bin Ji1, Jing-Ya Wang2
1 Research Center of Life Sciences and Environmental Sciences, Institute of Materia Medica, Harbin University of Commerce; Engineering Research Center of Natural Anticancer Drugs of Ministry of Education, Harbin University of Commerce, Harbin, Heilongjiang, China
2 Department of Pharmacy, School of Pharmacy, Harbin University of Commerce, Harbin, Heilongjiang, China
3 Research Center of Life Sciences and Environmental Sciences, Institute of Materia Medica, Harbin University of Commerce, Harbin, Heilongjiang, China
Correspondence Address:
Wen-Lan Li
School of Pharmacy, Harbin University of Commerce, Tongda 138, Daoli District, Harbin 150076
China
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/pm.pm_402_18
|
|
|
Background: Cistanche deserticola, a traditional Chinese herb medicine, has been widely used for thousands of years with the activities of hormone regulation, immunomodulatory, antioxidative, neuroprotective, anti-inflammatory, and estrogen. Glycosides of Cistanches (GCs) were the main bioactivity components of the herb. Objective: The objective of the study is to study estrogenic activity and the mechanism about estrogen receptors (ERs) of GCs. Materials and Methods: Cell proliferation was measured using the 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyltetrazolium bromide assay for MCF-7 cells. The cell cycle was detected using flow cytometry, and proliferation index was calculated. The mRNA and protein expressions of ERα and ERβ were detected by reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis as the reported method with minor modifications. Results: GCs group at the concentrations of 1.75, 17.5, and 175 μg/mg could enhance proliferation of the MCF-7 cell lines with a time and dosage-dependent manner. Combined medication group (fulvestrant with estradiol [E2] or GCs) could lead to the incline of proliferation rate compared with the individual medication group (P < 0.01). Flow cytometry analysis indicated that GCs could advance MCF-7 cell lines from G0/G1 phase cells to S and G2/M phase, which could promote cell DNA synthesis. The mechanism of GCs on MCF-7 was similar to that of E2. RT-PCR and western blot analysis indicated that after treatment with GCs for 48 h, contents of ERα and ERβ mRNA and proteins in MCF-7 increased as a dosage-dependent manner with that of GCs. GCs can play a role of estrogenic activity according upregulated mRNA and proteins of ERα and ERβ. Conclusion: This study indicated the estrogenic activity of GCs, and also, ER is the target of GCs. GCs can play a role of estrogenic activity according upregulated mRNA and proteins of ERα and ERβ.
|
|
|
|
| [FULL TEXT] [PDF]* |
|
 |
|
