Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
Advanced search 
Year : 2019  |  Volume : 15  |  Issue : 65  |  Page : 682-692

Bioactivity-guided isolation of cytotoxic and antioxidant phytochemicals from four Cousinia species from stenocephala bunge section

1 Department of Pharmacognosy, Faculty of Pharmacy, Erciyes University, Kayseri, Turkey
2 Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Ankara, Turkey
3 Department of Pharmacology, Faculty of Pharmacy, Erciyes University, Kayseri, Turkey
4 Department of Pharmaceutical Botany, Faculty of Pharmacy, Selcuk University, Konya, Turkey
5 Faculty of Pharmacy, Eastern Mediterranean University, Famagusta, North Cyprus via Mersin-10, Turkey

Correspondence Address:
Leyla Pasayeva
Department of Pharmacognosy, Faculty of Pharmacy, Erciyes University, Kayseri 38030
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/pm.pm_487_18

Rights and Permissions

Background: Asteraceae family contains several cytotoxic compounds bearing genus. Cousinia genus is included in the Asteraceae; it has not been studied phytochemically in detail. Objective: In this study, chemical compositions of four Cousinia species (Cousinia davisiana [CD], Cousinia foliosa, Cousinia ramosissima, and Cousinia stenocephala [CS]) were evaluated according to their cytotoxic and antioxidant effects using bioactivity-guided isolation. Materials and Methods: The cytotoxic effect was investigated with Sulphorhodamine B method against Colo205 (human colon carcinoma), A549 (human non-small cell lung cancer) cell lines, and antioxidant activity tested with 1,1-diphenyl-2-picrylhydrazyl, 2,2'-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid scavenging tests, and β-carotene/linoleic acid co-oxidation test. Purified compounds were elucidated by one-dimensional and two-dimensional nuclear magnetic resonance and mass spectroscopic techniques. The quantitative and qualitative determination of unpurified compounds within the extracts was carried out by liquid chromatography-mass spectrometry/mass spectrometry. Results: CS methanol extract, dichloromethane subextract, and FR-3 showed more cytotoxicity; isolated compound (ψ-taraxasterol) showed no cytotoxic activity. CD methanol extract and n-butanol subextract showed significant antioxidant activity. Conclusion: This is the first report that these phytochemical compounds were identified in Cousinia genus, and it is thought that these compounds could contribute to the chemotaxonomy of the genus.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded281    
    Comments [Add]    
    Cited by others 7    

Recommend this journal