| ORIGINAL ARTICLE |
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| Year : 2019 | Volume
: 15
| Issue : 60 | Page : 43-51 |
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Ameliorative effect of morin, a plant flavonoid against Freund's complete adjuvant-induced polyarthritis in rats
Guozhi Zhang1, Amit D Kandhare2, Anwesha A Mukherjee2, Subhash L Bodhankar2, Hailei Yin3
1 Department of Bone and Trauma Surgery, The Second People's Hospital of Yunnan Province, Kunming, Yunnan, India
2 Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Pune, Maharashtra, India
3 Department of Orthopedics, 401 Hospital of Chinese PLA, Qingdao, Shandong, China
Correspondence Address:
Hailei Yin
Department of Orthopedics, 401 Hospital of Chinese PLA, Qingdao, Shandong, 266071
China
Subhash L Bodhankar
Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Erandwane, Paud Road, Pune - 411 038, Maharashtra
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/pm.pm_351_18
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Background: Rheumatoid arthritis is a chronic relapsing autoimmune disorder with multifactorial etiology and prognosis. Morin [2-(2,4-dihydroxyphenyl)-3,5,7-trihydroxy-4H-1-benzopyran-4-one], a plant flavonoid reported having antioxidant and anti-inflammatory potential. Objective: The aim is to study the anti-arthritic activity of the morin against adjuvant-induced polyarthritis in rats. Materials and Methods: Polyarthritis was induced in female Wistar rats (150–180 g) using Freund's complete adjuvant (FCA) in the tail. Leflunomide (10 mg/kg, as a standard) and morin (10, 30 and 100 mg/kg) were administered orally daily for 28 days. Results: Treatment with morin (30 and 100 mg/kg) showed statistically significant inhibition (P < 0.05) in FCA-induced decrease in thermal hyperalgesia and mechanical hyperalgesia. It also showed significant attenuation (P < 0.05) in FCA-induced alterations in hematological parameters, aspartate aminotransferase, alanine transaminase, alkaline phosphatase, serum turbidity, albumin, C-reactive protein, blood sugar levels, and erythrocyte sedimentation rate. Altered levels of serum and liver lipid profile were significantly attenuated (P < 0.05) by morin (30 and 100 mg/kg). Morin treatment significantly decreased (P < 0.05) serum oxido-nitrosative stress, tumor necrosis factor-alpha (TNF-α), and interleukin-1β (IL-1β) levels. Morin (30 and 100 mg/kg) also significantly (P < 0.05) inhibited FCA-induced up-regulated liver TNF-α, IL-1β, IL-6, heme oxygenase-1, transforming growth factor-beta and down-regulated nuclear factor erythroid 2-related factor-2 messenger RNA expression. Histopathology alteration-induced by FCA was also reduced by morin treatment. Conclusion: Morin showed promising curative properties against FCA-induced polyarthritis in rats via modulation of endogenous biomarkers. Thus, morin can be considered as an alternative treatment regimen for the management of arthritis.
Abbreviations used: AIA: Adjuvant-induced arthritis, ALT: Alanine transaminase, ALP: Alkaline phosphatase, AST: Aspartate aminotransferase, BSL: Blood glucose level, CRP: C-reactive protein, ESR: Erythrocyte sedimentation rate, FCA: Freund's complete adjuvant, HO: Heme oxygenase, HDL: High-density lipoprotein, IL-1β: Interleukin-1 beta, LDL: Low-density lipoprotein, MDA: Malondialdehyde, NO: Nitric oxide, Nrf2: Nuclear factor-like 2, ROS: Reactive oxygen species, GSH: Reduced glutathione, RT-PCR: Reverse transcription-polymerase chain reaction, SOD: Superoxide dismutase, TC: Total cholesterol, TGF-β: Transforming growth factor-beta, TG: Triglyceride, TNF-α: Tumor necrosis factor-alpha, VLDL: Very low-density lipoprotein.
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