|Year : 2018 | Volume
| Issue : 58 | Page : 534-540
Antioxidant and anti-inflammatory effects of a methanol extract from the marine sponge Hyrtios erectus
Ramachandran Muthiyan1, Nilkamal Mahanta2, Balwin Nambikkairaj3, Titus Immanuel4, Arun Kumar De5
1 Department of Zoology, Voorhees College, Thiruvalluvar University, Vellore, Tamil Nadu; Bioinformatics Centre, Central Island Agricultural Research Institute, Port Blair, Andaman and Nicobar Islands, India
2 Department of Chemistry and Institute For Genomic Biology, University of Illinois, Urbana Champaign, Illinois, USA
3 Department of Zoology, Voorhees College, Thiruvalluvar University, Vellore, Tamil Nadu, India
4 Division of Fisheries Sciences, Central Island Agricultural Research Institute, Port Blair, Andaman and Nicobar Islands, India
5 Bioinformatics Centre; Department of Animal Sciences, Central Island Agricultural Research Institute, Port Blair, Andaman and Nicobar Islands, India
|Date of Submission||10-Apr-2017|
|Date of Acceptance||25-Apr-2017|
|Date of Web Publication||21-Nov-2018|
Arun Kumar De
Department of Animal Sciences, Central Island Agricultural Research Institute, Port Blair - 744 101, Andaman and Nicobar Islands
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background: The marine environment, due to its phenomenal diversity, is a rich natural source of many biologically active compounds. Objective: Marine sponge Hyrtios erectus, collected from North Bay of South Andaman Sea, was screened for potential antioxidant and anti-inflammatory activities. Materials and Methods: The antioxidant activities of the methanol (MeOH) extract of the sponge at different concentrations (0–100 μg/mL) were determined by measuring the free radical-scavenging activities. The anti-inflammatory activities of the extract were determined by measuring the inhibitory effect of the extract on albumin denaturation and inducible nitric oxide (NO) production. Quantitative real-time polymerase chain reaction was used to investigate the effect of the sponge extract on the expression of eight proinflammatory cytokine genes. Results: Our results suggested that the MeOH extract of the sponge exhibited antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl-free radicals, superoxide anions, and hydroxyl radicals. More than 50% inhibition (half inhibitory concentration) was recorded with concentration of 50 μg/mL of the sponge extract. Extract of the sponge at a concentration of 25 μg/mL inhibited NO production by a macrophage cell line in vitro by 91.22% ± 5.78%. The sponge extract induced downregulation of eight proinflammatory cytokine genes in breast cancer Michigan Cancer Foundation-7 cell line. Conclusion: The secondary metabolites present in the MeOH extract of the sponge showed the potential antioxidant and anti-inflammatory activities. Further studies are required to identify the bioactive compounds.
Abbreviations used: DPPH: 2,2-diphenyl-1-picrylhydrazyl; DMEM: Dulbecco's modified eagle's medium; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; EDTA: Ethylenediaminetetraacetic acid; NBT: Nitroblue tetrazolium; PMS: Phenazine methosulfate; DMSO: Dimethyl sulfoxide.
Keywords: Antioxidant, marine sponge, proinflammatory cytokines, tumor necrosis factor
|How to cite this article:|
Muthiyan R, Mahanta N, Nambikkairaj B, Immanuel T, De AK. Antioxidant and anti-inflammatory effects of a methanol extract from the marine sponge Hyrtios erectus. Phcog Mag 2018;14:534-40
|How to cite this URL:|
Muthiyan R, Mahanta N, Nambikkairaj B, Immanuel T, De AK. Antioxidant and anti-inflammatory effects of a methanol extract from the marine sponge Hyrtios erectus. Phcog Mag [serial online] 2018 [cited 2022 Nov 26];14:534-40. Available from: http://www.phcog.com/text.asp?2018/14/58/534/245840
- Marine sponge Hyrtios erectus, collected from North Bay, South Andaman Sea, India, showed potential antioxidant and anti-inflammatory activities
- Methanol extract of the sponge exhibited antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl-free radicals, superoxide anions, and hydroxyl radicals
- Extract of the sponge, at a concentration of 25 μg/mL, inhibited nitric oxide production by a macrophage cell line in vitro by 91.22% ± 5.78%
- The sponge extract induced downregulation of eight proinflammatory cytokine genes in Michigan Cancer Foundation-7 cell line.
| Introduction|| |
Marine environment is being recognized to be a very rich natural source of bioactive metabolites with potent pharmacological importance. Biological diversity of certain marine ecosystems, such as coral reefs, is higher than that of tropical rain forests. Many marine organisms including sponges have a sedentary lifestyle and evolved the ability to produce diverse toxic compounds to protect themselves from predators or to fight against competitors., Among all marine organisms, sponges are getting most attention in pharmaceutical industry because of the vast majority of bioactive secondary metabolites; they produce anticancer, antiviral, antibacterial, antifungal, antiprotozoal, anti-inflammatory, immunosuppressive, neurosuppressive, neuroprotective, and a range of other bioactivities. It is reported that almost 40% of all marine natural products come from sponges. The major sources of most of the sponge-derived compounds are from the microorganisms which harbor on their surfaces and intercellular spaces., Bioactive compounds produced by sponges vary considerably between different species and locations, ranging from some that produce no compound to others that produce large amount of the compounds.
Free radicals damage cells and antioxidants inhibit the production of free radicals by blocking the oxidation of other molecules. Bioactive compounds with antioxidant potential have been reported from marine sponges., Secondary metabolites derived from marine sponges also showed anti-inflammatory activities. Some examples of sponge-derived metabolites with anti-inflammatory activities include cavernolide from Fasciospongia cavernosa, contignasterol from Petrosia contignata, and cyclolinteinone from Cacospongia linteiformis.
Research on marine sponges for the identification of bioactive compounds has been intensified over the last decade. However, there are very few reports on the screening of sponge species from North Bay of South Andaman Sea, India. The marine sponge Hyrtios erectus is blackish and is attached to the sea bottom by means of masses of sand-filled fibers. However, this sponge species from South Andaman Sea water has not been investigated before. Therefore, the purpose of the present study was to investigate the potential antioxidant and anti-inflammatory effects of the secondary metabolites produced by the sponge H. erectus. We used the methanol (MeOH) extract of the sponge for the purpose. The effect of the sponge extract on the expression of eight proinflammatory cytokine genes was also investigated.
| Materials and Methods|| |
All the present experiment complies with all relevant institutional and national animal welfare guidelines and policies.
All chemicals were purchased from Sigma-Aldrich (USA) unless stated otherwise. Cell culture plastics were from MIDSCI, USA.
Sample collection and preparation of the methanol extract
The marine sponge H. erectus was sampled in North Bay of South Andaman Sea, India, in December 2013 through scuba diving. The taxonomy details of the sponge samples were studied thoroughly, and a voucher specimen was deposited to Division of Fisheries Sciences, ICAR-Central Island Agricultural Research Institute, Andaman and Nicobar Islands.
The bioactive compounds were extracted using MeOH. The extract was filtered through Whatman filter paper No. 1 (GE Healthcare, UK) and pooled into a rotary evaporator (STRIKE 202, Germany). Then, the filtrate was concentrated under reduced pressure below 50°C into a thick mass. The crude extract was redissolved in MeOH:CHCl3(1:1) mixture and was partitioned into four solvents (dichloromethane, MeOH, chloroform, and hexane), followed by evaporation of the aqueous layer. All the fractions were subjected to bioassays (scavenging assays and nitric oxide [NO] production assay as described below) to determine the antioxidant and anti-inflammatory activities. Among the four fractions, MeOH-soluble fractions showed that antioxidant and anti-inflammatory activities and other fractions were found ineffective. On the basis of the results, the MeOH-soluble fraction was taken for the current study. The MeOH extract was dried and stored at −20°C until use.
2,2-diphenyl-1-picrylhydrazyl-free radical-scavenging assay
The 2,2-diphenyl-1-picrylhydrazyl (DPPH)-free radical-scavenging activity of sponge extracts at different concentrations (5, 15, 25, 50, and 100 μg/mL) was measured as described previously. Briefly, 0.1 mM solution of DPPH was added to 3 mL of the sponge extract solutions and stirred vigorously. Then, each mixture was kept in the dark for 30 min, the absorbance was measured at 517 nm against a blank, and the percentage inhibition was calculated using the following equation.
where A0 is the absorbance of the control (solution without sponge extract) and A1 is the absorbance of the sponge extract samples.
Superoxide anion-scavenging assay
Measurement of superoxide anion-scavenging activity of sponge extract was based on the method described previously. The sponge extract solutions were incubated with 1 mL of nitroblue tetrazolium (NBT) reaction mixture containing phosphate buffer (20 mM, pH 7.4), nicotinamide adenine dinucleotide (reduced form, 73 μM), NBT (50 μM), and phenazine methosulfate (PMS, 15 μM) for 5 min at ambient temperature. Subsequently, the absorbance at 560 nm was measured against an appropriate blank containing everything, except the sponge extract. The inhibition % was calculated using equation 1.
Hydroxyl radical-scavenging assay
The scavenging activity of the sponge extract for hydroxyl radicals was measured by the method previously reported. The reaction mixture was prepared by mixing 2-deoxy-2-ribose (2.8 mM), phosphate buffer (0.1 mM, pH 7.4), ferric chloride (20 μM), ethylenediaminetetraacetic acid (EDTA, 100 μM), hydrogen peroxide (500 μM), ascorbic acid (100 μM), and various concentrations (0–100 μg/mL) of the sponge extract solution in a final volume of 1 mL. After incubating the reaction mixture for 1 h, an aliquot of the reaction mixture (0.8 mL) was added to 2.8% trichloroacetic acid solution (1.5 mL), followed by thiobarbituric acid solution (1% in 50 mM sodium hydroxide, 1 mL) and sodium dodecyl sulfate (0.2 mL). The mixture was then heated (20 min at 90°C) to allow the color to develop. After cooling, the absorbance was measured at 532 nm against an appropriate blank solution including all reaction components but the sponge extract. The percentage of inhibition was calculated using equation 1.
In vitro anti-inflammatory assay
In vitro anti-inflammatory activity of the sponge extract was determined by its ability to inhibit albumin denaturation and to inhibit NO formation by the macrophages.
Inhibition of in vitro albumin denaturation
Inhibition of in vitro albumin denaturation by the sponge extract was assessed as described previously. In brief, 2 mL of 1 mM bovine serum albumin (BSA) solution was mixed with 2 mL of the sponge extract solutions. The mixtures were incubated at 27°C in an incubator for 15 min followed by incubation at 60°C in water bath for 10 min for denaturation. Then, the mixture was allowed to cool and turbidity was measured spectrophotometrically at 660 nm. The percentage of inhibition of denaturation was calculated using equation 2.
Inhibition of nitric oxide production
Cell lines and cell culture
The mouse macrophage cell line (RAW 264.7) was obtained from National Centre for Cell Sciences (NCCS), Pune, India. The cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, 100 mU/mL penicillin, and 100 μg/mL streptomycin. Cell cultures were incubated in a humidified atmosphere of 5% CO2 in air and 37°C.
The cells were plated at a density of 2 × 105 cells/well in a 24-well plate. After incubation for 24 h, the cells were stimulated with 1 μg/mL of lipopolysaccharide (LPS) in the presence and absence of various concentrations of sponge extract for 24 h. The concentration of nitrite, a stable metabolite of NO was measured in the culture supernatant by the Griess assay as described previously.
Cell viability assessment
To ensure that the inhibition of nitrite production was not due to cytotoxicity of the sponge extract, the cytotoxic potential of the sponge extract on RAW 264.7 cells was tested using (3- [4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide) MTT assay. For the MTT assay, RAW 264.7 cells were seeded in a 96-well plate in basal medium (DMEM + 10% FBS + antibiotics), and after 24 h of adherence, the cells were treated with various concentrations (0–100 μg/mL) of the sponge extract and incubated for 24 h at 37°C in an atmosphere of 5% CO2 in air. After the incubation period, the medium was removed and 100 μL MTT solution (1 mg/mL) was added to each well and the samples were allowed to incubate for 4 h under condition mentioned above. Then, the MTT-containing media were removed and the formazan crystals were dissolved with 200 μL of dimethyl sulfoxide. Absorbance was measured at 570 nm using a microplate reader. The cell viability percentage was calculated as described previously.
Effect of the sponge extract on the expression of proinflammatory cytokine genes in breast carcinoma cell line (Michigan Cancer Foundation-7)
Cell lines and cell culture
The breast adenocarcinoma cell line (Michigan Cancer Foundation [MCF]-7) was obtained from NCCS, Pune, India. The cells were cultured in DMEM supplemented with 10% FBS, 2 mM glutamine, 100 mU/mL penicillin, and 100 μg/mL streptomycin. Cell cultures were incubated in a humidified atmosphere of 5% CO2 in air and 37°C.
To determine the effect of the sponge extract on expression of proinflammatory cytokine mRNAs, MCF-7 cells were cultured in DMEM with 10% FBS in the presence of different concentrations of the sponge extract. Cells cultured in the absence of sponge extract were used as the control. After 24 h, the cells were harvested and washed in PBS and total RNA was extracted. Expression of eight proinflammatory cytokine genes, i.e., tumor necrosis factor-α (TNF-α), interferon-λ (IFNG), interleukin-6 (IL6), interleukin-1α (IL1A), interleukin-1β (IL1B), chemokine (C-C Motif) ligand 2 (CCL2), chemokine (C-X-C Motif) ligand 8 (CXCL8), and chemokine (C-X-C Motif) ligand 10 (CXCL10) were studied by quantitative real-time polymerase chain reaction analysis (PCR).
RNA preparation and reverse transcription-quantitative polymerase chain reaction analysis
Total RNA was extracted from MCF-7 cells that were either untreated (control) or exposed to various concentrations of the sponge extract (5, 15, 25, 50, and 100 μg/mL) using the RNAeasy Mini Kit (Qiagen Inc., Valencia, CA, USA) according to the manufacturer's instructions. Quality of the RNA was determined using a NanoDrop spectrophotometer, and the concentration of the RNA was determined by Qubit® RNA HS Assay Kit (Thermo Fisher Scientific, Life Technologies) as per the manufacturer's protocol.
Reverse transcription of RNA was performed from 1 μg total RNA in the presence of RNase inhibitor, random hexamer primers (50 ng/μL), deoxynucleotides (10 mM), SuperScript III reverse transcriptase (200 U/μL), and reverse transcriptase buffer in a 20 μL final reaction volume using SuperScript III First-Strand Synthesis System for RT-PCR Kit (Invitrogen, Life Technologies, IN, USA).
Relative quantification of the genes was performed using Power SYBR green PCR Master Mix (2X) (Applied Biosystems) in Taqman ABI 7900 Real-Time PCR System (Applied Biosystems). Relative expressions of eight proinflammatory cytokine genes (TNF, IFNG, IL6, IL1A, IL1B, CCL2, CXCL8, and CXCL10) were studied. The housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as the endogenous control. The thermal cycling conditions for real-time PCR were one cycle of 50°C for 2 min (AmpErase uracil-N-glycosylase activation) and 95°C for 10 min (AmpliTaq Gold activation), followed by 40 cycles of 95°C for 15 s (denaturation) and 60°C for 1 min (annealing and extension). Fold changes were determined using the 2−ΔΔCt method. Primer sequences used in this experiment are provided in [Table 1].
|Table 1: Information on primers used in the real-time polymerase chain reaction|
Click here to view
All the statistical analysis was performed by one-way analysis of variance with Dunnett's posttest. Data were expressed as mean ± standard deviation. GraphPad Prism version 6.0 (GraphPad Software, San Diego, CA, USA) was used to analyze the data.
| Results|| |
In vitro antioxidant activity of the sponge extract
In the present experiment, in vitro antioxidant activity of the sponge extract was evaluated by DPPH-free radical-scavenging assay, superoxide anion-scavenging assay, and hydroxyl radical-scavenging assay. In vitro antioxidant activity of sponge extract in various concentrations is presented in [Table 2]. More than 50% inhibition (half inhibitory concentration [IC50]) was recorded with concentration of 50 μg/mL of the sponge extract.
The DPPH-free radical-scavenging effects of the sponge extract at different concentrations are shown in [Table 2]. Sponge extract at all chosen concentrations showed free radical-scavenging activity in a concentration-dependent manner. Sponge extract was able to reduce the DPPH to give a percentage of inhibition 19.02 ± 2.65, 21.67 ± 1.53, 33.67 ± 4.04, 56.33 ± 4.52, and 66.01 ± 2.05 at concentrations of 5, 15, 25, 50, and 100 μg/mL, respectively. The IC50 was below 50 μg/mL.
The superoxide anion radical-scavenging activity of the sponge extract at different concentrations is presented in [Table 2]. A concentration-dependent scavenging pattern was also observed in this case. The percent inhibition of superoxide anion was 26.33 ± 1.53, 29.67 ± 4.51, 37.33 ± 2.52, 59.67 ± 6.66, and 68.32 ± 4.35 at concentrations of 5, 15, 25, 50, and 100 μg/mL, respectively. The IC50 was below 50 μg/mL.
The hydroxyl radical-scavenging assay of the sponge extract at different concentrations is presented in [Table 2]. The inhibition of hydroxyl radical was 17.67% ±4.04%, 31.00% ±3.6%, 37.34% ±1.53%, 53.02% ±1.25%, and 69.67% ±2.08% at concentrations of 5, 15, 25, 50, and 100 μg/mL, respectively. The IC50 was below 50 μg/mL.
In vitro anti-inflammatory activity
As denaturation of proteins is a well-known cause of inflammation, the ability of the sponge extract to inhibit BSA denaturation was investigated and the percent inhibition of protein denaturation is presented in [Figure 1]. The sponge extract inhibited the denaturation of BSA in a concentration-dependent manner. The inhibition of BSA denaturation was 11.67% ± 1.53%, 16.33% ± 1.57%, 29.34% ± 2.08%, 47.00% ± 2.21%, and 76.56% ± 2.53% at concentrations of 5, 15, 25, 50, and 100 μg/mL of sponge extract, respectively, with IC50 of 55 μg/mL.
|Figure 1: Inhibition of denaturation of bovine serum albumin by the sponge extract. Inhibition of in vitro albumin denaturation was used to evaluate the anti-inflammatory activity of the sponge extract. The values and error bar represent average and standard deviation of three independent sets of experiments. Diclofenac acid was used as the standard|
Click here to view
The inhibitory activity of the extract on NO production by induced macrophage cell line (RAW 264.7) is presented in [Table 3]. The inhibition of NO production was 67.94% ± 8.64%, 86.45% ± 5.39%, 91.22% ± 5.78%, 91.09% ± 4.67%, and 87.57% ± 6.34% at concentrations of 5, 15, 25, 50, and 100 μg/mL of sponge extract, respectively. The viability of the RAW 264.7 macrophages was 88.34% ± 5.33%, 86.08% ± 3.84%, 91.65% ± 5.11%, 89.30% ± 3.18%, and 86.52% ± 4.31% at concentrations of 5, 15, 25, 50, and 100 μg/mL of sponge extract, respectively. The viability of the RAW 264.7 macrophages was not significantly lower than in nontreated cells, indicating that the inhibition of the NO production was not due to cytotoxicity of the sponge extract.
|Table 3: Inhibitory activity of sponge extract on lipopolysaccharide-activated nitric oxide production in RAW 264.7 macrophages|
Click here to view
Effect of the sponge extract on proinflammatory cytokine expression in breast carcinoma cell line (Michigan cancer foundation-7)
Proinflammatory cytokines are produced predominantly by activated macrophages, and they promote systemic inflammation. The effect of the sponge extract on the expression of the proinflammatory cytokine genes (TNF, IFNG, IL6, IL1A, IL1B, CCL2, CXCL8, and CXCL10) in MCF-7 cells is presented in [Figure 2]. Following treatment, expressions of all the proinflammatory cytokines were downregulated. Significant decrease in the expressions of IL1A, IL1B, and CCL2 was observed at a sponge concentration as low as 15 μg/mL, while the reduction in the expression level of the rest of the proinflammatory cytokines required a little higher concentration of the sponge extract (25 μg/mL) [Figure 2]. The results of this study demonstrated that the sponge extract contains some bioactive compounds which have the potential to inhibit the expression levels of proinflammatory cytokines.
|Figure 2: Effect of the sponge extract on the expression of proinflammatory cytokine transcripts in Michigan cancer foundation-7 Cells Real-time reverse transcription polymerase chain reaction analysis was performed to analyze the expression of eight proinflammatory cytokine genes. (a) Tumor Necrosis factor, alpha (TNF), (b) interferon, gamma (IFNG), (c) interleukin-6 (IL-6), (d) interleukin-1, alpha (IL1A), (e) interleukin-1, beta (IL1B), (f) chemokine (C-C Motif) ligand 2 (CCL2), (g) chemokine (C-X-C Motif) ligand 8 (CXCL8), and (h) chemokine (C-X-C Motif) ligand 10 (CXCL10). The values and error bars represent average and standard deviations of three independent sets of experiments. One-way analysis of variance followed by Dunnett posttest was performed to find out significant difference between control and treatments. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001|
Click here to view
| Discussion|| |
There is increasing evidence that suggest that the marine environment contains different classes of biologically active compounds with strong antioxidant and anti-inflammatory activities. Marine sponges are very rich source of structurally diverse natural products including alkaloids, steroids, terpenes, peptides, macrolides, and polyketides. Several screening investigations have demonstrated that crude extracts from marine sponges display potent antioxidant and anti-inflammatory properties., In the present work, screening of the antioxidant and anti-inflammatory effects of the MeOH extract of the sponge H. erectus isolated from North Bay of South Andaman Sea, India, was carried out. The sponge extract exhibited excellent antioxidant and anti-inflammatory activities and induced downregulation of eight proinflammatory cytokine transcripts in a breast carcinoma cell line (MCF-7).
A free radical contains an unpaired electron in its atomic orbital. Free radicals are involved in some signal pathways; thereby are beneficial at moderate levels, but at higher concentrations cause damage to our body by oxidative stress. Antioxidants inhibit the production of free radicals by blocking the oxidation of other molecules. In the present study, it was found that the MeOH extract of the sponge could act as a potential antioxidant. The sponge extract resulted in >50% inhibition of free radicals, superoxide radicals, and hydroxyl radicals at a concentration of 50 μg/mL [Table 2]. A number of metabolites derived from marine sponges, such as indole derivatives, aromatic alkaloids, aromatic polyketides, and phenolic compounds, have exhibited strong antioxidant potential., Aromatic polyketides isolated from the marine sponge-derived fungus, Aspergillus versicolor, have shown significantly higher antioxidant capacity than that of butylated hydroxytoluene. In another study, with the Caribbean sponge, Pandaros acanthifolium, the isolated steroidal glycosides exhibited high antioxidant and cytoprotective activities.
In the present study, the MeOH extract of the sponge showed potential anti-inflammatory effect. It showed inhibitory effect on the denaturation of BSA in vitro[Figure 1] and on NO production by LPS-induced macrophages [Table 3]. NO promotes inflammation; therefore, any compound which shows inhibitory effect on NO production can be used as a potential anti-inflammatory compound. There are several reports on anti-inflammatory effect of marine sponge extracts.,, Several sponge metabolites with anti-inflammatory activities have been reported; such as cavernolide from F. cavernosa, contignasterol from P. contignata, and cyclolinteinone from C. linteiformis. Although the mechanism of anti-inflammatory effect varies with sponge species, phospholipase A2 inhibition has been reported to be the major mechanism of anti-inflammatory properties of several sponges of order Dictyoceratida.,
Costantini et al., 2015 reported that the extract of the sponge Geodia cydonium induced the decrease of proinflammatory cytokines in breast cancer MCF-7 cells, indicating that MCF-7 cell line can be used as an in vitro model to study the anti-inflammatory effect of any compound. In the present study, the effect of the sponge extract on the expression of eight proinflammatory cytokine genes in MCF-7 cells was evaluated. It was found that the sponge extract downregulated the transcripts of eight proinflammatory cytokines [Figure 2], further supporting our results on its potential anti-inflammatory properties. IL1β, IL6, and TNF-α are among the most widely investigated proinflammatory cytokines. IFNγ stimulates cytotoxic T-cells, augments TNF activity, and induces NO production. CCL2 and CCL8 are mediators of acute inflammation and stimulate a variety of cells including macrophages, monocytes, lymphocytes, eosinophils, and basophils.,, Reduction of the proinflammatory cytokine transcripts on treatment with the sponge extract [Figure 2] indicates the presence of bioactive compounds with potent anti-inflammatory activity.
Marine sponges of the genus Hyrtios have been well known as a rich source of bioactive compounds. Antiproliferative and proapoptotic activities of marine sponge H. erectus extract on breast carcinoma cell line have been reported. MeOH extract of the sponge showed cytotoxicity against three cancer cell lines HepG2, A549, and PC-3. A bioactive metabolite (24methoxypetrosaspongia C) from Hyortios sp. showed growth inhibitory activity against hepatocellular carcinoma, colorectal carcinoma, and breast adenocarcinoma cells, whereas two alkaloids (hyrtinadines C and D) from Hyortios sp. showed potential antimicrobial and antifungal activities.
| Conclusion|| |
The screening of the marine sponge H. erectus from North Bay of South Andaman Sea, India, suggested that it is a rich source of compounds with antioxidant and anti-inflammatory properties. The secondary metabolites produced by the sponge induced downregulation of the proinflammatory cytokine transcripts. The bioactive compounds responsible for the activities need to be identified, and functional characterization of the compounds is required. This study will further add to the repertoire of bioactive compounds isolated from marine sponges and will make them potential candidates for therapeutic use in the future.
Financial support and sponsorship
Department of Biotechnology, under Sub-distributed Information Centre (Project number: BT/BI/04/066/2004), India, supported this research work.
Conflicts of interest
There are no conflicts of interest.
| References|| |
Dellai A, Deghrigue M, Laroche-Clary A, Masour HB, Chouchane N, Robert J, et al.
Evaluation of antiproliferative and anti-inflammatory activities of methanol extract and its fractions from the mediterranean sponge. Cancer Cell Int 2012;12:18.
Manivasagan P, Venkatesan J, Kim SK. Marine Microbiology: Bioactive Compounds and Biotechnological Applications. Weinheim, Germany:Wiley Publication; 2013.
Paul VJ, Puglisi MP, Ritson-Williams R. Marine chemical ecology. Nat Prod Rep 2006;23:153-80.
Hussain MS, Fareed S, Ansari S, Khan MS. Marine natural products: A lead for anti-cancer. Indian J Geomarine Sci 2012;41:27-39.
Blunt JW, Copp BR, Munro MH, Northcote PT, Prinsep MR. Marine natural products. Nat Prod Rep 2005;22:15-61.
Blunt JW, Copp BR, Hu WP, Munro MH, Northcote PT, Prinsep MR, et al.
Marine natural products. Nat Prod Rep 2007;24:31-86.
Fusetani N, Kem W. Marine toxins: An overview. Prog Mol Subcell Biol 2009;46:1-44.
Miller JH, Singh AJ, Northcote PT. Microtubule-stabilizing drugs from marine sponges: Focus on peloruside A and zampanolide. Mar Drugs 2010;8:1059-79.
Page M, West L, Northcote P, Battershill C, Kelly M. Spatial and temporal variability of cytotoxic metabolites in populations of the New Zealand sponge Mycale hentscheli
. J Chem Ecol 2005;31:1161-74.
Utkina NK. Antioxidant activity of aromatic alkaloids from marine sponges Aaptos aaptos
sp. Chem Nat Compd 2009;6:849-53.
Longeon A, Copp BR, Quévrain E, Roué M, Kientz B, Cresteil T, et al.
Bioactive indole derivatives from the South Pacific marine sponges Rhopaloeides odorabile
sp. Mar Drugs 2011;9:879-88.
Posadas I, Terencio MC, De Rosa S, Payá M. Cavernolide: A new inhibitor of human sPLA2 sharing unusual chemical features. Life Sci 2000;67:3007-14.
Burgoyne DL, Andersen RJ, Allen TM. Contignasterol, a highly oxygenated steroid with the ‘unnatural’ 14β configuration from the marine sponge Petrosia contignata thiele
. J Org Chem 1992;57:525-8.
D'acquisto F, Lanzotti V, Carnuccio R. Cyclolinteinone, a sesterterpene from sponge Cacospongia linteiformis
, prevents inducible nitric oxide synthase and inducible cyclo-oxygenase protein expression by blocking nuclear factor-kappaB activation in J774 macrophages. Biochem J 2000;346 Pt 3:793-8.
Salmoun M, Devijver C, Daloze D, Braekman JC, van Soest RW. 5-hydroxytryptamine-derived alkaloids from two marine sponges of the genus hyrtios. J Nat Prod 2002;65:1173-6.
Kedare SB, Singh RP. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol 2011;48:412-22.
Hazra B, Biswas S, Mandal N. Antioxidant and free radical scavenging activity of Spondias pinnata
. BMC Complement Altern Med 2008;8:63.
Ozyürek M, Bektaşoğlu B, Güçlü K, Apak R. Hydroxyl radical scavenging assay of phenolics and flavonoids with a modified cupric reducing antioxidant capacity (CUPRAC) method using catalase for hydrogen peroxide degradation. Anal Chim Acta 2008;616:196-206.
Sathe BS, Jagtap VA, Deshmukh SD, Jain BV. Screening of in vitro
anti-inflammatory activity of some newly synthesized fluorinated benzothiazolo imidazole compounds. Int J Pharm Sci 2011;3:220-2.
Kiemer AK, Vollmar AM. Elevation of intracellular calcium levels contributes to the inhibition of nitric oxide production by atrial natriuretic peptide. Immunol Cell Biol 2001;79:11-7.
Freshney RI. Culture of Animal Cells – A Manual of Basic Technique and Specialized Applications. 6th
ed. New Jersey: Hoboken; 2010.
Upton G, Cook I. A Dictionary of Statistics. 2nd
ed. Oxford, United Kingdom: Oxford University Press; 2006.
Mehbub MF, Lei J, Franco C, Zhang W. Marine sponge derived natural products between 2001 and 2010: Trends and opportunities for discovery of bioactives. Mar Drugs 2014;12:4539-77.
Dias DA, Urban S, Roessner U. A historical overview of natural products in drug discovery. Metabolites 2012;2:303-36.
Costantini S, Romano G, Rusolo F, Capone F, Guerriero E, Colonna G, et al.
Anti-inflammatory effects of a methanol extract from the marine sponge Geodia cydonium
on the human breast cancer MCF-7 cell line. Mediators Inflamm 2015;2015:204975.
Shaaban M, Abd-Alla HI, Hassan AZ, Aly HF, Ghani MA. Chemical characterization, antioxidant and inhibitory effects of some marine sponges against carbohydrate metabolizing enzymes. Org Med Chem Lett 2012;2:30.
Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev 2010;4:118-26.
Rao PS, Kalva S, Yerramilli A, Mamidi S. Free radicals and tissue damage: Role of antioxidants. Free Radic Antioxid 2011;1:2-7.
Li Z. Advances in marine microbial symbionts in the China Sea and related pharmaceutical metabolites. Mar Drugs 2009;7:113-29.
Berrué F, McCulloch MW, Boland P, Hart S, Harper MK, Johnston J, et al.
Isolation of steroidal glycosides from the Caribbean sponge Pandaros acanthifolium
. J Nat Prod 2012;75:2094-100.
Adebayo SA, Dzoyem JP, Shai LJ, Eloff JN. The anti-inflammatory and antioxidant activity of 25 plant species used traditionally to treat pain in Southern African. BMC Complement Altern Med 2015;15:159.
Azevedo LG, Peraza GG, Lerner C, Soares A, Murcia N, Muccillo-Baisch AL, et al.
Investigation of the anti-inflammatory and analgesic effects from an extract of Aplysina caissara
, a marine sponge. Fundam Clin Pharmacol 2008;22:549-56.
Kim YK, Na KS, Myint AM, Leonard BE. The role of pro-inflammatory cytokines in neuroinflammation, neurogenesis and the neuroendocrine system in major depression. Prog Neuropsychopharmacol Biol Psychiatry 2016;64:277-84.
Schroder K, Hertzog PJ, Ravasi T, Hume DA. Interferon-gamma: An overview of signals, mechanisms and functions. J Leukoc Biol 2004;75:163-89.
Gerszten RE, Garcia-Zepeda EA, Lim YC, Yoshida M, Ding HA, Gimbrone MA Jr., et al.
MCP-1 and IL-8 trigger firm adhesion of monocytes to vascular endothelium under flow conditions. Nature 1999;398:718-23.
Zlotnik A, Yoshie O. Chemokines: A new classification system and their role in immunity. Immunity 2000;12:121-7.
Rose CE Jr., Sung SS, Fu SM. Significant involvement of CCL2 (MCP-1) in inflammatory disorders of the lung. Microcirculation 2003;10:273-88.
Muthiyan R, Nambikkairaj B, Mahanta N, Immanuel T, Mandal RS, Kumaran K, et al.
Antiproliferative and proapoptotic activities of marine sponge Hyrtios erectus
extract on breast carcinoma cell line (MCF-7). Pharmacogn Mag 2017;13:S41-S47.
Alarif WM, Al-Lihaibi SS, Ghandourah MA, Orif MI, Basaif SA, Ayyad SE, et al.
Cytotoxic scalarane-type sesterterpenes from the Saudi Red Sea sponge Hyrtios erectus
. J Asian Nat Prod Res 2016;18:611-7.
Elhady SS, El-Halawany AM, Alahdal AM, Hassanean HA, Ahmed SA. A new bioactive metabolite isolated from the red sea marine sponge Hyrtios erectus
. Molecules 2016;21:82.
Kubota T, Nakamura K, Sakai K, Fromont J, Gonoi T, Kobayashi J, et al.
Hyrtinadines C and D, new azepinoindole-type alkaloids from a marine sponge Hyrtios
sp. Chem Pharm Bull (Tokyo) 2016;64:975-8.
[Figure 1], [Figure 2]
[Table 1], [Table 2], [Table 3]
|This article has been cited by|
||Recent Progress in Antioxidant Active Substances from Marine Biota
| ||Todorka Vladkova, Nelly Georgieva, Anna Staneva, Dilyana Gospodinova |
| ||Antioxidants. 2022; 11(3): 439 |
|[Pubmed] | [DOI]|
||Chemical Evaluation, Antioxidant, Antiproliferative, Anti-Inflammatory and Antibacterial Activities of Organic Extract and Semi-Purified Fractions of the Adriatic Sea Fan, Eunicella cavolini
| ||Dario Matulja, Petra Grbcic, Krunoslav Bojanic, Natalija Topic-Popovic, Rozelindra Což-Rakovac, Sylvain Laclef, Tomislav Šmuc, Ozren Jovic, Dean Markovic, Sandra Kraljevic Pavelic |
| ||Molecules. 2021; 26(19): 5751 |
|[Pubmed] | [DOI]|